The invention discloses a
molecular identification method for organic
sulfate in PM2.5 based on LC-MS / MS. The
molecular identification method is characterized by comprising the following steps: firstly, carrying out ultrasonic-assisted extraction on an atmospheric
fine particulate matter sample by adopting
ultrapure water, carrying out
centrifugal separation, taking supernate, and filtering through a 0.45 mu m microporous filter membrane; then purifying the filtrate through a
solid-phase extraction column, and purging and concentrating the collected eluent through
nitrogen until the eluent is in a near-dry state to complete the enrichment of a target object; an LC-MS / MS combined
electrospray ionization source (ESI) is adopted to perform
mass spectrometric analysis on organic
sulfate in atmospheric
fine particulate matters in a negative
ion mode, and structure confirmation of a target compound is realized. Compared with direct adoption of high-
resolution mass spectrometry (FT-ICR MS), the method has the advantages that the sample pretreatment process is simplified, the analysis efficiency is remarkably improved, the powerful separation
advantage and the high reliability
advantage of LC-MS / MS are fully played, the organic
sulfate in a
complex matrix can be accurately identified, high-
throughput and high-selectivity detection of the organic sulfate in the PM2.5 sample is achieved, and the method is suitable for large-scale popularization and application. The structure confirmation of the organic sulfate can be realized, and an analysis and confirmation integrated
molecular identification method for the organic sulfate in the PM2.5 sample is provided.