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Fucoidan-galactosan sulfate, extracting, separating, and purifying method thereof, and application thereof

A galactan, separation and purification technology, applied in the field of biochemistry, to achieve the effect of high value and broad market prospects

Active Publication Date: 2011-11-09
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the analysis of the chemical structure and activity research of this kind of sulfated heteropolysaccharides has always been the research bottleneck of this kind of polysaccharides.

Method used

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  • Fucoidan-galactosan sulfate, extracting, separating, and purifying method thereof, and application thereof
  • Fucoidan-galactosan sulfate, extracting, separating, and purifying method thereof, and application thereof
  • Fucoidan-galactosan sulfate, extracting, separating, and purifying method thereof, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: Preparation of Fucogalactan Sulfate

[0041] (1) Remove silt from 5 kg of kelp, extract in a pressure cooker with water 20 times the weight of kelp for 3 hours, control the temperature at 100-105°C, and extract twice. Remove the algae, combine the two extracts, filter with diatomaceous earth, concentrate the filtrate, add 4 times the volume of the concentrate for precipitation, and filter to obtain crude polysaccharides. The yield of crude polysaccharides is 4.84%.

[0042] (2) Dissolving the crude polysaccharide in water to form a solution with a mass concentration of 1.5%, adding 2mol / L MgCl 2 Make MgCl 2 The final mass concentration is 0.05mol / L, add absolute ethanol at the same time, make the final weight concentration of ethanol be 20%, stir to generate precipitate, centrifuge to remove the precipitate, take supernatant and add 95% ethanol, make the final weight concentration of ethanol be 70-75% %, stirring to form a precipitate, centrifuging to co...

Embodiment 2

[0044] Example 2: Structural Identification of Fucogalactan Sulfate

[0045] (1) Desulfurization reaction of fucogalactan sulfate.

[0046] First, pretreat the HZ001 resin with 1mol / L hydrochloric acid-pyridine, then weigh 1.0g of fucogalactan sulfate and dissolve it in 50mL of deionized water, wash the HZ001 resin with distilled water for 10 column volumes The washed fractions were collected, concentrated, adjusted to neutral pH with pyridine, and freeze-dried polysaccharide pyridine salt. Dissolve 0.5 g of the above polysaccharide pyridinium salt in 100 mL of dimethyl sulfoxide-methanol-pyridine solution (volume ratio 89:10:1), and react at 100° C. for 9 h. After the reaction is finished, the reaction solution is dialyzed, freeze-dried, and the degree of desulfation is detected by determining the content of sulfuric acid groups and infrared spectroscopy.

[0047] (2) Methylation analysis of fucogalactan sulfate

[0048] a. Reagent handling

[0049] Take 25 g of molecular...

Embodiment 3

[0060] Example 3: Therapeutic effect of fucogalactan sulfate on rats with chronic renal failure

[0061] 1. Model making of chronic renal failure

[0062] Adult male Wistar rats of SPF grade were fed with ordinary feed for observation for one week after purchase. After the general condition was good, 10 rats were randomly taken out as the normal control group (normal saline 10mL / kg), and the remaining 50 rats were divided into groups immediately after weighing. 300mg / kg / d adenine was administered orally by gavage once a day, and the model was established continuously for 21 days. After modeling, blood was collected, 2-3mL from each rat, and placed in the laboratory for 2 hours after blood collection, centrifuged at 3000 rpm for 10 minutes, and the supernatant was taken to detect the content of blood creatinine and urea nitrogen. According to the experimental results, take After modeling, the animals were divided into 5 groups, 10 animals in each group.

[0063] 2. Model anim...

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Abstract

The invention discloses a fucoidan-galactosan sulfate with a novel structure. Fucoidan-galactosan sulfate provided by the present invention is a sulfated polysaccharide composed of fucose and galactose. According to the chemical constitution of the sugar chain, a fundamental chain is formed by -4-alpha-L-Fuc-(1,3)-alpha-L-Fuc-(1,3)-alpha-L-Fuc-(1,3)-alpha-L-Fuc-(1-residue. Every 4 sugar residues comprise a branched chain, which is composed of alpha-L-Fuc(1- or beta-D-Gla-(1,6)-beta-D-Gla-(1-. Sulfated group is connected to the 2 or 4 site of fucose, or on the 3 or 4 site of galactose. Meanwhile, the invention discloses a preparation method and an application of fucoidan-galactosan sulfate. Fucoidan-galactosan sulfate provided by the present invention can be exploited into medicines for treating kidney diseases.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a fucogalactan sulfate ester, a separation and purification method and application thereof. Background technique [0002] Fucoidan, also known as fucoidan sulfate, English name fucan or fucoidan, is a large class of sulfated polysaccharides composed of fucose existing in marine brown algae or marine invertebrates. It has anticoagulant, antitumor, anti Thrombosis, anti-inflammatory and other biological activities. Now people have a clear understanding of the composition of fucogalactan, which is a kind of polysaccharide with very complex chemical composition and structure. [0003] The chemical structure of fucoidan is very complex, and the structure of fucoidan isolated from different brown algae is very different. So far, the structure of fucoidan from Fucus vesiculosus and Ascophyllum nodosum has been studied the most. Fucus fucoidan is mainly linked by α(1→3) glycosidic bonds. c...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/737A61P13/12A61P3/10
Inventor 张全斌王晶张忠山张虹牛锡珍
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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