Technique for extracting sulfate polysaccharide from Laminaria digitata

A technology of sulfated polysaccharides and kelp, which is applied in the field of extraction technology, can solve the problems of destroying the structure of sulfated polysaccharides, difficulty in meeting production needs, and the impact of sulfate content, so as to retain structure and stability, save ethanol consumption, and improve extraction rate Effect

Active Publication Date: 2014-09-10
ZHEJIANG YUXIANG BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional and commonly used methods for extracting sulfated polysaccharides mainly include acid extraction, alkali extraction and water extraction, etc. Among them, hot water extraction is mostly used. Acid and alkali extraction will destroy the structure of sulfated polysaccharides, and will also affect the content of sulfate radicals. ; while the extraction rate of hot water extraction is generally low, it is difficult to meet the production needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A process for extracting sulfated polysaccharides from kelp, comprising the steps of:

[0032] (1) The kelp is washed, dried, crushed and passed through a 270-mesh sieve, and then placed in the extraction reactor;

[0033] (2) Add a certain volume of water into the extraction reactor, the pH is 5, and the liquid-solid ratio is 45:1. Use ultrasonic-assisted extraction for a period of time to obtain the extract, and centrifuge to obtain the supernatant; among them, the ultrasonic power is 120W, and the ventilation Amount of 75L / h extraction temperature is 65 ℃, extraction time is 28min;

[0034] (3) Add protease to the supernatant to decompose protein into amino acids, and then concentrate through nanofiltration membrane to 1 / 3 of the original volume to obtain crude polysaccharide concentrate; among them, the feeding amount of protease is 0.7% of the weight of kelp, and the enzymatic hydrolysis time for 8h;

[0035] (4) Add ethanol to the crude polysaccharide concentrat...

Embodiment 2

[0037] A process for extracting sulfated polysaccharides from kelp, comprising the steps of:

[0038] (1) The kelp is washed, dried, crushed and passed through a 150-mesh sieve, and then placed in the extraction reactor;

[0039] (2) Add a certain volume of water into the extraction reactor, the pH is 4.5, and the liquid-solid ratio is 40:1. After a period of time, ultrasonic-assisted extraction is used to obtain the extract, and the supernatant is obtained by centrifugation; among them, the ultrasonic power is 110W, and the ventilation Volume 80L / h extraction temperature is 65°C, extraction time is 26min;

[0040] (3) Add protease to the supernatant to decompose protein into amino acids, and then concentrate through nanofiltration membrane to 1 / 4 of the original volume to obtain crude polysaccharide concentrate; among them, the feeding amount of protease is 0.9% of the weight of kelp, and the enzymatic hydrolysis time 7.5h;

[0041] (4) Add ethanol to the crude polysacchari...

Embodiment 3

[0043] A process for extracting sulfated polysaccharides from kelp, comprising the steps of:

[0044] (1) The kelp is washed, dried, crushed and passed through a 230-mesh sieve, and then placed in the extraction reactor;

[0045] (2) Add a certain volume of water into the extraction reactor, the pH is 5.5, and the liquid-solid ratio is 48:1. Use ultrasonic-assisted extraction for a period of time to obtain the extract, and centrifuge to obtain the supernatant; among them, the ultrasonic power is 130W, and the ventilation Amount of 72L / h extraction temperature is 60°C, extraction time is 25min;

[0046] (3) Add protease to the supernatant to decompose protein into amino acids, first filter through an ultrafiltration membrane with a molecular weight cut-off of 1000-3000, and then concentrate through a nanofiltration membrane to 1 / 5 of the original volume to obtain a crude polysaccharide concentrate; among them, The feeding amount of protease is 1.3% of the weight of kelp, and t...

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PUM

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Abstract

The invention relates to a technique for extracting sulfate polysaccharide from Laminaria digitata, which comprises the following steps: cleaning Laminaria digitata, drying, pulverizing, adding a certain volume of water, carrying out ultrasonic assisted extraction for some time to obtain an extracting solution, centrifugating, adding proteinase into the supernate to decompose protein into amino acid, concentrating with a nanofiltration membrane to 1 / 3-1 / 5 of the original volume to obtain a crude polysaccharide concentrated solution, adding ethanol into the crude polysaccharide concentrated solution until the volume percent is 20-40%, standing, centrifugating, concentrating the centrifugate with a nanofiltration membrane to 1 / 2-1 / 3 of the original volume, adding ethanol until the volume percent is 70-85%, and carrying out centrifugal separation to obtain the sulfate polysaccharide. The technique is simple, and adopts the membrane to filter out water and amino acid, thereby saving the ethanol amount for the next purification step; and meanwhile, compared with the traditional heating concentration, the membrane filter concentration technique effectively keeps the structure and stability of the polysaccharide, and enhances the extraction rate of the sulfate polysaccharide.

Description

technical field [0001] The invention relates to an extraction process, in particular to a process for extracting sulfated polysaccharides from kelp. Background technique [0002] Polysaccharides widely exist in animal cell membranes and plant and microbial cell walls. They are high molecular polymers linked by aldehyde groups and ketone groups through glycoside bonds. They are also one of the four basic substances that constitute life. In the past 20 years, with the continuous deepening of natural medicinal chemistry and pharmacological research, that is, the rapid development of analytical methods, the study of polysaccharides has aroused great interest from many scholars at home and abroad. Studies have shown that natural polysaccharides have anti-tumor and immune-enhancing effects. Therefore, the important biological effects of immune polysaccharides in natural animals and plants have attracted more and more attention, and become a hot research topic today. [0003] Sul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘振锋戴圣佳吕卫金黄小鸣叶兴乾
Owner ZHEJIANG YUXIANG BIOLOGICAL SCI & TECH
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