Technique for extracting sulfate polysaccharide from Laminaria digitata

Abstract

The invention relates to a technique for extracting sulfate polysaccharide from Laminaria digitata, which comprises the following steps: cleaning Laminaria digitata, drying, pulverizing, adding a certain volume of water, carrying out ultrasonic assisted extraction for some time to obtain an extracting solution, centrifugating, adding proteinase into the supernate to decompose protein into amino acid, concentrating with a nanofiltration membrane to 1 / 3-1 / 5 of the original volume to obtain a crude polysaccharide concentrated solution, adding ethanol into the crude polysaccharide concentrated solution until the volume percent is 20-40%, standing, centrifugating, concentrating the centrifugate with a nanofiltration membrane to 1 / 2-1 / 3 of the original volume, adding ethanol until the volume percent is 70-85%, and carrying out centrifugal separation to obtain the sulfate polysaccharide. The technique is simple, and adopts the membrane to filter out water and amino acid, thereby saving the ethanol amount for the next purification step; and meanwhile, compared with the traditional heating concentration, the membrane filter concentration technique effectively keeps the structure and stability of the polysaccharide, and enhances the extraction rate of the sulfate polysaccharide.

Description

technical field [0001] The invention relates to an extraction process, in particular to a process for extracting sulfated polysaccharides from kelp. Background technique [0002] Polysaccharides widely exist in animal cell membranes and plant and microbial cell walls. They are high molecular polymers linked by aldehyde groups and ketone groups through glycoside bonds. They are also one of the four basic substances that constitute life. In the past 20 years, with the continuous deepening of natural medicinal chemistry and pharmacological research, that is, the rapid development of analytical methods, the study of polysaccharides has aroused great interest from many scholars at home and abroad. Studies have shown that natural polysaccharides have anti-tumor and immune-enhancing effects. Therefore, the important biological effects of immune polysaccharides in natural animals and plants have attracted more and more attention, and become a hot research topic today. [0003] Sul...

AI Technical Summary

Problems solved by technology

The traditional and commonly used methods for extracting sulfated polysaccharides mainly include acid extraction, alkali extraction and water extraction, etc. Among them, hot water extraction is mostly used. Acid and alkali extraction will destroy the structure of sulfated polysaccharides, and will also affect the content of sulfate radicals. ; while the extraction rate of hot water extraction is generally low, it is difficult to meet the production needs