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Enzymatical detection method of saccharify blood albumen as well as liquid stabilising agent

A technology of glycosylated serum protein and determination method, which is applied in the field of liquid stable reagents, and can solve problems such as short validity period, waste, and pollution of automatic biochemical analyzers

Inactive Publication Date: 2008-07-23
温州市第三人民医院
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Among them, the nitrotetrazolium blue colorimetric method has poor accuracy and serious pollution to the biochemical automatic analyzer; although the ketoamine oxidase method is accurate and simple, they are all freeze-dried powder reagents, which are expensive and have a period of validity after reconstitution. Short, serious waste, difficult to carry out in clinical practice

Method used

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  • Enzymatical detection method of saccharify blood albumen as well as liquid stabilising agent

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Embodiment

[0025] Example: glycosylated serum protease method two-point endpoint method assay reagent, the buffer solution in component A is particularly preferably pH6.5MES buffer solution, the buffer solution in component B is particularly preferably MOPOS-MOPOSNa buffer solution; the particularly preferred surface in component A The active agent is Tween80, and the particularly preferred surfactant in the B component is TritonX-100; the particularly preferred stabilizer in the A component is aspartic acid; the particularly preferred stabilizer in the B component is bovine serum albumin; The preservative is preferably PC300. In the above reagent composition, calcium chloride is used to activate bromelain, and Tween80 and TritonX-100 can keep the measurement system clear during the measurement process, avoiding the abnormal increase of the absorbance of the measurement system caused by protein turbidity.

[0026] Component A:

[0027] pH6.5MES buffer 40mmol / L

[0028] Bromelain 10KU / L...

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Abstract

The invention relates to an enzymatic measuring method of glycosylated serum protein and a relative liquid stabilizer, with wide application in medical and biochemical technical field. The invention is characterized in that glycosylated serum protein is digested by bromelain to generate fructose amino acid, fructose amino acid oxidase is reacted with the fructose amino acid to generate H2O2 to be reacted with chromogen DA-64 in the presence of peroxidase to convert DA-64 into one green product, the product content is in direct proportion with glycosylated serum protein content of sample, and an automatic biochemical analyzer uses two-point end point method to measure the content of glycosylated serum protein in the sample. The inventive liquid measuring agent has high stability, easy application, strong interference resistance and non pollution on the pipeline of automatic biochemical analyzer, which is suitable for automatically measuring glycosylated serum protein in batch.

Description

technical field [0001] The invention relates to a fructose amino acid oxidase automatic analysis method for glycosylated serum protein and a liquid stable reagent thereof, which can be widely used in the technical fields of medicine and biochemistry. Background technique [0002] Glycated serum protein is a glycosylated protein with a ketamine chain formed by non-enzymatic glycation reactions on the N-terminal amino groups of glucose and albumin and other protein molecules in the blood, also known as fructosamine, and is a routine clinical test item. Glycated serum protein concentration can effectively reflect the average blood sugar level of patients in the past 1-3 weeks, and is not affected by temporary fluctuations in blood sugar concentration. index. Therefore, accurate determination of the content of glycosylated serum protein has important clinical value. [0003] At present, the clinical methods for the determination of glycosylated serum protein are mainly the nit...

Claims

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Application Information

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IPC IPC(8): G01N33/68C12Q1/28G01N31/16G01N21/78
Inventor 陈益川
Owner 温州市第三人民医院
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