30 results about "Bromelains" patented technology

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention discloses modified hydroxytyrosol as well as a preparation method, cosmetics and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, hydroxytyrosol and bromelain are mixed for an enzymatic hydrolysis reaction, and the modified hydroxytyrosol is prepared. According to the provided preparation method of the modified hydroxytyrosol, the hydroxytyrosol is subjected to catalytic modification through bromelain, bromelain serves as typical sulfydryl protease and is subjected to an enzymolysis reaction with the hydroxytyrosol, the hydroxytyrosol enzymolysis derivative is generated, and the generated hydroxytyrosol enzymolysis derivative contains various substances with aromatic smells, so that the hydroxytyrosol can be used for preparing the modified hydroxytyrosol. The obvious pungent smell of the hydroxytyrosol raw material is effectively improved.

The invention provides tooth enamel repairing toothpaste and a preparation method thereof, and the tooth enamel repairing toothpaste comprises the following components in percentage by mass: 15-30% of glycerol, 15-30% of glycerol, 5-10% of sodium dodecyl sulfate, 5-10% of sodium dodecyl sulfate, 5-10% of sodium dodecyl sulfate and the balance of deionized water. 10%-25% of hydrated silica; 3%-10% of polyethylene glycol-8; 3%-7% of calcium phosphosilicate; 1%-5% of a surfactant; 0.5%-3% of a binder; 0.05%-0.5% of an antibacterial agent; 0.1%-1.5% of a compound enzyme; the compound enzyme comprises lysozyme, papain and bromelain; the mass ratio of the lysozyme to the papain to the bromelain is 1: (0.4-0.6): (0.4-0.6); and the balance of water. The toothpaste can be used for remarkably improving the repairing effect of enamel and the oral health maintenance capability.

The invention belongs to the technical field of biological enzyme preservation, and particularly relates to a bromelain freeze-drying protective agent and a preparation method thereof. The trehalose and pulullan compound freeze-dried powder injection is prepared from the following components in parts by weight: 30 to 50 parts of trehalose, 8 to 10 parts of pulullan, 3 to 10 parts of maltodextrin, 12 to 22 parts of mannitol, 1 to 4 parts of sorbitol, 4 to 12 parts of glycine, 0.05 to 0.2 part of polysorbate-20, 1.5 to 4.5 parts of a buffering agent, 0.2 to 0.8 part of N-acetylcysteine, 0.05 to 0.3 part of oxidized glutathione, 0.8 to 2.5 parts of PEG (Polyethylene Glycol) and 0.05 to 0.3 part of an antioxidant. According to the invention, trehalose-pullulan is taken as a main skeleton, so that the growth of ice crystals is inhibited, and the water activity is reduced; mannitol constructs a crystal phase support network, and polysorbate-20 stabilizes an interface; sulfydryl is temporarily sealed through oxidized glutathione, and controllable reduction during redissolution is realized through microencapsulated N-acetylcysteine; and a sodium phosphate buffer system keeps the pH stable.

The present application belongs to the technical field of biological enzyme preservation, and particularly relates to a bromelain freeze-drying protective agent and a preparation method thereof. The composition comprises the following components: 30-50 parts of trehalose, 8-10 parts of pullulan, 3-10 parts of malt dextrin, 12-22 parts of mannitol, 1-4 parts of sorbitol, 4-12 parts of glycine, 0.05-0.2 parts of polysorbate-20, 1.5-4.5 parts of a buffer, 0.2-0.8 parts of N-acetylcysteine, 0.05-0.3 parts of oxidized glutathione, 0.8-2.5 parts of PEG and 0.05-0.3 parts of an antioxidant. The present application takes trehalose-pullulan polysaccharide as a main skeleton, inhibits ice crystal growth and reduces water activity; mannitol constructs a crystal phase support network, and polysorbate-20 stabilizes the interface; oxidized glutathione temporarily seals mercapto groups, and microencapsulated N-acetylcysteine realizes controllable reduction during redissolution; and a sodium phosphate buffer system maintains pH stability.

The present application relates to a kind of methods for preparing high-purity ecdysone from dew grass, comprising (1) pretreatment, (2) enzymolysis, the complex enzyme includes cellulase, pectinase, papain and bromelain;(3) extraction, (4) purification: dew grass extract is passed through macroporous adsorption resin, sequentially using alkali washing, one water washing, acid washing, twice water washing, ethanol elution, concentration;(5) bleaching, (6) crude crystallization, (7) recrystallization.The purity and yield of β-ecdysone product obtained by the method of the present application are high, and the composite enzyme enzymolysis is combined with ultrasonic countercurrent extraction, which can efficiently and quickly extract;During the purification process, low-concentration alkali washing and acid washing can effectively elute impurities, improve the purity of β-ecdysone, and also reduce the difficulty of macroporous resin regeneration, improve production efficiency.The method of the present application is simple, and the resin and solvent used can be reused, with low process cost, suitable for industrial production.

The invention provides a marine bioactive protein peptide and application thereof in fresh keeping of chicken. The application of the marine bioactive protein peptide in fresh keeping of chicken comprises the following steps: S1, cutting chicken breast into blocks; s2, performing enzymolysis on the shrimp meat with bromelain and chymotrypsin, performing centrifugation, and adding lactic acid into supernate to prepare marine bioactive protein peptide; s3, adding the marine bioactive protein peptide into the hydroxypropyl methyl cellulose solution to prepare a fresh-keeping solution loaded with the marine bioactive protein peptide; and S4, soaking the blocky chicken breast in a fresh-keeping solution, taking out, draining, performing vacuum packaging, and performing cold storage. The fresh-keeping liquid loaded with marine bioactive protein peptides is used for keeping the chicken breast fresh, the oxidation process of chicken is delayed, water loss is remarkably reduced, and the sensory quality of chicken is kept.

The invention relates to a light-operated genetically engineered bacterium knapsack and application thereof, and belongs to the technical field of genetic engineering and biological medicine crossing, the genetically engineered bacterium knapsack comprises an engineered bacterium, and the engineered bacterium is prepared by transferring a recombinant plasmid expressing a chemotactic factor CCL21 and a recombinant plasmid expressing cleavage protein into an initial strain escherichia coli; the surface of the engineering bacterium is modified with bromelain and PGA-DA-Cys. The genetically engineered bacterium knapsack can be anchored on the surface of macrophages so as to target tumor tissues, and the genetically engineered bacterium knapsack can significantly activate immune response of tumor parts so as to achieve a tumor immunotherapy effect.

The invention relates to a preparation method of a rhodomyrtus tomentosa fruit extract as well as a product and application of the rhodomyrtus tomentosa fruit extract. The preparation method comprises the following steps: mixing a rhodomyrtus tomentosa fruit raw material with a compound enzyme for enzymolysis, adding an ethanol aqueous solution into an enzymolysis reaction solution after enzymolysis is completed, performing negative pressure cavitation extraction, filtering and collecting filtrate to obtain the rhodomyrtus tomentosa fruit extract, the compound enzyme comprises a combination of cellulase, bromelain, alpha-amylase and tannase. According to the method, the effective components in the longan pulp are extracted by adopting the specific compound enzyme, the related components in the rhodomyrtus tomentosa fruits can be efficiently extracted, a potential synergistic relationship exists among bromelain, alpha-amylase and tannase, the extraction effect can be remarkably improved after the bromelain, the alpha-amylase and the tannase are combined, and the method is suitable for industrial production. The prepared extract product has the effects of resisting inflammation, relieving, resisting oxidation and the like.

The present application relates to a kind of preparation method of immunoglobulin powder and its application, the preparation method includes the following steps: collecting bovine colostrum, using cell survival technology is frozen;Frozen bovine colostrum is thawed, carries out milk fat separation, and obtains defatted bovine colostrum;Defatted bovine colostrum and composite rennet are mixed and are curdled, and then broken, obtain whey;The composite rennet includes aspartic protease, papain and bromelain;Whey is ultrafiltrated, and immunoglobulin solution is obtained;Immunoglobulin solution is vacuum freeze-dried, and the immunoglobulin powder is obtained.The preparation method provided by the present application is low temperature all the way, the activity of immunoglobulin is retained to the greatest extent, the use of composite rennet solves the problem of curd failure, and each link cooperates to improve the curd effect, realizes the preparation of high-efficiency, high-purity and high-quality immunoglobulin powder.

A method for treating and / or improving inflammatory intestinal disease includes administering a composition to a patient suffering from inflammatory intestinal disease to be capable of effectively inhibiting the patient's intestinal bleeding, intestinal inflammation, and capable of achieving efficacies of repairing intestinal cells and intestinal wall structure. The composition contains an effective amount of bromelain and / or an effective amount of Bidens pilosa extract.

The invention belongs to the technical field of livestock and poultry by-product processing, and particularly relates to an efficient tenderization method for livestock and poultry by-products. The method comprises the following steps: preparing a papain solution and a bromelain solution by taking natural small molecule water as a solvent, and tenderizing the livestock and poultry byproducts by adopting the papain solution and the bromelain solution. Natural small molecule water is used for dissolving papain and bromelain for directional efficient tenderization, the problem of processing quality of livestock and poultry byproducts can be solved, and uniformity of internal and external taste of the byproducts is effectively achieved.

The invention discloses a whey protein peptide with high anticoagulant activity and a blood fat reducing effect and a preparation method thereof. The preparation method comprises the following steps: firstly carrying out pulsed electric field pretreatment on a whey protein aqueous solution, then hydrolyzing the pretreated whey protein aqueous solution by using bromelain, and freeze-drying to obtain the whey protein peptide. According to the method, bromelain is selected to carry out enzymolysis on the whey protein aqueous solution, and the whey protein aqueous solution is pretreated by using the pulsed electric field before enzymolysis, so that the use of the pulsed electric field is beneficial to promoting the exposure of peptide chain segments with anticoagulation and blood fat reduction functions, and the anticoagulation and blood fat reduction capabilities of the whey protein peptide are remarkably improved. The method breaks through the limitation of the traditional process, and overcomes the problems of low efficiency and limited active peptide release of a single enzymolysis method through the synergistic effect of the pulsed electric field pretreatment and the enzymolysis technology. The non-thermal effect of the pulsed electric field can directionally change the space structure of the whey protein, more enzyme cutting sites are exposed, and meanwhile protein denaturation caused by high-temperature treatment is avoided.

The invention discloses toothpaste and a preparation method thereof. The toothpaste is prepared from components in percentage by mass as follows: 8%-15% of bamboo salt, 0.03%-0.15% of o-cymene-5-alcohol, 1%-5% of polyglycerol fatty acid ester, 0.5%-3% of calcium salt, 0.05%-0.3% of lysozyme, 0.2%-1% of bromelain, 15%-28% of a friction agent, 0.4%-1.2% of a thickening agent, 0.1%-2% of a pH buffer system, 10%-35% of a humectant, 1.5%-4% of a foaming agent and the balance of water. Glycyrrhizic acid is replaced by polyglycerol ester / calcium ion micelles, so that the problem of IPMP inactivation is thoroughly solved while the pH conflict is avoided.

The application provides a preparation method and application of a bromelain composition, and belongs to the technical field of biotechnology.The composition contains bromelain, superoxide dismutase, amino acids and complex polysaccharides extracted from aloe, sturgeon collagen peptide and hamamelis extract; the aloe amino acid polysaccharide, sturgeon collagen peptide and hamamelis extract are high-efficiency products obtained through special enzymolysis or fermentation and purification. The various components are used in combination, can remove dead skin cells, remove wrinkles, keep the skin firm and elastic, have good whitening and anti-wrinkle effects, and have few component types, do not cause absorption burden to the skin, and are safe and efficient.

The application discloses a quercetin-eucalyptus oil composition for clearing and protecting lungs, and relates to the technical field of functional food. The composition comprises an active functional component and a three-phase layered targeting delivery system. The active functional component is composed of quercetin 5-20 parts, eucalyptus oil 1-8 parts, bromelain 2-10 parts, dihydromyricetin 10-30 parts, perillaldehyde 1-6 parts, icariin 3-15 parts and ultralow-molecular-weight sodium hyaluronate 1-5 parts in terms of weight fraction. The three-phase layered targeting delivery system comprises three independent embedding units, namely enteric-soluble protective microspheres for wrapping bromelain, nano-mixed micelles for wrapping quercetin, dihydromyricetin and icariin, and slow-release microcapsules for wrapping eucalyptus oil and perillaldehyde. The application solves the problems of component compatibility conflict and component inactivation in traditional compositions by scientifically matching seven active functional components and combining the three-phase layered targeting delivery system, improves the utilization rate of components, can be produced on a large scale, and improves the storage stability of the composition.

The invention discloses a preparation method of highland barley protein peptide with hypoglycemic activity, and belongs to the field of biotechnology and functional food. According to the preparation method, highland barley protein is extracted from highland barley powder by adopting an alkali-solution and acid-isolation method, then the highland barley protein peptide is prepared by sequentially adopting alkaline protease and bromelain to carry out step-by-step enzymolysis, and the complementary and synergistic effects of different restriction enzyme cutting sites are realized through a step-by-step enzymolysis strategy of'alkaline protease-bromelain 'in a specific sequence. Aromatic and hydrophobic amino acids are preferentially cut by the alkaline protease, the space structure of the protein is opened, and more effective sites are provided for bromelain, so that small molecular peptide fragments with hypoglycemic activity are more efficiently released, and in-vitro experiments show that the inhibition rate of the bromelain on alpha-amylase is up to 52.49%, and the inhibition rate of the bromelain on the bromelain is up to 52.49%. The inhibition rate of alpha-glucosidase reaches up to 73.35%, which is far better than that of a single enzymolysis product.

The present invention discloses a quercetin-eucalyptus-oil composition for clearing and protecting the lungs, relating to the technical field of functional foods. The composition comprises active functional components and a three-phase layered targeted-delivery system. The active functional components comprise, in parts by weight, 5-20 parts quercetin, 1-8 parts eucalyptus oil, 2-10 parts bromelain, 10-30 parts dihydromyricetin, 1-6 parts perillaldehyde, 3-15 parts icariin, and 1-5 parts ultra-low-molecular-weight sodium hyaluronate. The three-phase layered targeted-delivery system comprises three independent encapsulation units, namely enteric-protective microspheres encapsulating bromelain, nano mixed micelles encapsulating quercetin, dihydromyricetin, and icariin, and sustained-release microcapsules encapsulating eucalyptus oil and perillaldehyde. By scientifically combining the seven active functional components with the three-phase layered targeted-delivery system and by improving the preparation process, the present invention solves the problems of component incompatibility and easy inactivation in conventional compositions, improves ingredient utilization, enables large-scale production, and enhances the storage stability of the composition.

The present application relates to a kind of skin regeneration repair medicine and preparation method thereof, belong to biological medicine technical field, and medicine includes oil palm extraction peptide, tiger palm silver ear extract, Gangdeng root-milletia nitida-Siberian milkvetch composite extract and pomegranate peel polyphenol etc.Oil palm extraction peptide is oil palm sequentially by cellulase, alpha-chymotrypsin and ficin enzymolysis, then by G-50 dextran gel column purification and obtain the product above 1kDa;Tiger palm silver ear extract is tiger palm silver ear fruit body by cellulase and bromelain common enzymolysis, and obtain the product below 3kDa;Gangdeng root-milletia nitida-Siberian milkvetch composite extract is Gangdeng root, milletia nitida and siberian milkvetch by gradient extraction of ethanol aqueous solution and obtain extract, sequentially by polyamide resin column adsorption and LH-60 dextran gel column purification and obtain the product.Multiple components construct good repair system, accelerate wound repair healing.

The invention belongs to the technical field of protease, and particularly relates to composite bromelain and application thereof in a medicine for improving dyspepsia. The invention discloses compound bromelain. The compound bromelain is prepared by mixing bromelain, rhamnosidase and lipase according to the mass ratio of (1: 3)-(7: 1). The compound bromelain disclosed by the invention has a synergistic effect and can effectively enhance gastrointestinal motility, accelerate transmission and emptying of food in gastrointestinal tracts and improve dyspepsia symptoms caused by insufficient gastrointestinal motility, such as abdominal distension, early satiety and the like. Besides, the compound bromelain disclosed by the invention not only can maintain the self enzymatic activity, but also can synergistically promote the activity of other digestive enzymes and enhance the capability of decomposing various components in food, so that macromolecules in the food can be more effectively decomposed into micromolecular nutrient substances, the digestion efficiency is improved, and the digestion effect is improved. The dyspepsia caused by insufficient secretion or low activity of digestive enzymes is solved.

The invention relates to the technical field of food production, and discloses a production process for pickling chafing dish goose intestines with compound seasonings, which comprises the following steps: S1, microorganism synergistic fermentation pretreatment: soaking goose intestines in a low-salt solution containing lactic acid bacteria and salt, fermenting at 4-6 DEG C, washing with cold water after fermentation is completed, and draining; s2, biological enzyme pretreatment: soaking the goose intestines subjected to fermentation pretreatment in a bromelain solution, treating at 4-6 DEG C, washing with cold water after treatment is completed, and draining; s3, pulse ultrasonic assisted pickling; s4, performing vacuum sealing and low-temperature curing; and S5, closed-loop pickling material recovery. The method comprises the following steps: carrying out microbial synergistic fermentation pretreatment, fermenting for 1.5-2 hours by utilizing a low-salt solution of 1-2% v / v lactic acid bacteria and 2-3 parts by weight of salt, and adjusting the pH of the surface of the goose intestine to 6.0-6.5, so that the permeability of the seasoning is enhanced, the initial microbial load is reduced, and the food safety and flavor uniformity are ensured.

The present application relates to the technical field of in vitro detection, in particular to a rapid sperm morphology staining reagent and a staining method thereof; the rapid sperm morphology staining reagent of the present application can make semen spread rapidly and uniformly after 5 minutes by using bromelain and L-cysteine in combination. The staining reagent of the present application greatly shortens the pretreatment time of the sample and improves the detection efficiency.

The invention discloses a zero-degree indoor treatment fresh rice dumpling preparation method, and particularly relates to the technical field of fresh rice dumpling preparation, and the method comprises the following steps: S1, raw material pretreatment, S2, high-pressure variable-temperature cooking, S3, gradient temperature control cooling, and S4, zero-degree environment composite preservation. The sensory quality and eating experience of the fresh zongzi are improved through the technologies of fresh fruit microcapsule embedding, sticky rice quick-freezing-unfreezing pretreatment, high-pressure variable-temperature cooking, bromelain tenderization and the like, meanwhile, by means of a composite antibacterial film, modified atmosphere packaging, gradient temperature control cooling and a whole-process low-temperature cold chain, the preservation period is prolonged, and food safety is guaranteed; and the balance between industrial production and quality stability is realized through standardized pretreatment, cold chain parameter solidification and accurate regulation and control of the boiling process.

This invention discloses a biotechnology-based process and product for preparing deodorized mushroom concentrate, relating to the field of mushroom concentrate technology. The invention involves taking finely filtered mushroom broth, concentrating it, and controlling the solid content to obtain a mushroom concentrate. The mushroom concentrate is then adjusted to pH using sodium hydroxide or hydrochloric acid, heated, and immobilized bromelain and papain are added, stirred, and then immobilized flavor protease is added, stirred, and filtered to obtain the deodorized mushroom concentrate. Bromelain, papain, and flavor protease are selected. Bromelain and papain are endopeptidases that cleave long protein chains in mushrooms at different sites, providing more ends for the flavor protease. The flavor protease is an exopeptidase that hydrolyzes the protein fragments cleaved by bromelain and papain to form flavor amino acids. During the enzymatic hydrolysis process, the immobilized carriers of the three enzymes remove off-odors through adsorption.

The application relates to the technical field of biological separation engineering, and discloses a recombinant biomimetic affinity ligand for purifying bromelain as well as a preparation method and application of the recombinant biomimetic affinity ligand, which is prepared from the following raw materials in parts by weight: double-bonded silica-coated magnetic microspheres: 100 parts; monomer methyl acrylate: 150-250 parts; epoxy-containing monomer glycidyl methacrylate: 50-100 parts; crosslinking agent ethylene glycol dimethacrylate: 3-6 parts; and biomimetic affinity heptapeptide: 10-20 parts. The biomimetic affinity heptapeptide with a specific amino acid sequence is used as the affinity ligand, the hydrophobic residues and charged side chains in the sequence can specifically bind to the S2 sub-site and surrounding residues of the active center of bromelain to realize cavity complementation, high-specificity recognition of the target enzyme is realized, and the affinity medium prepared by using the ligand can effectively eliminate the interference of homologous impure proteins which are highly similar in structure and physicochemical properties.

The application discloses a method for preparing walnut polypeptide milk drink from low-temperature pressed walnut cake, and the method comprises the following steps: using low-temperature pressed walnut cake with a certain fat content as raw material, carrying out two-step enzymolysis, obtaining a walnut polypeptide solution after purification treatment, and then adding additives into the walnut polypeptide solution to obtain walnut polypeptide milk drink; wherein cellulase is used for the first-step enzymolysis, and bromelin is used for the second-step enzymolysis. The method for preparing walnut polypeptide milk drink from low-temperature pressed walnut cake has the advantages that the prepared walnut polypeptide milk drink is rich in walnut polypeptide, has good flavor and taste, good stability, is beneficial to human body digestion and absorption, and has physiological functions such as antioxidation and blood pressure reduction.

The invention discloses a separation and purification method of a bromelain hydrolase and high protein hydrolysis active peptide fragment mixture, and belongs to the technical field of biology. The method comprises the following steps: firstly, pretreating bromelain to obtain a bromelain aqueous solution without insoluble impurities, then loading a supernatant to a cation exchange column for gradient elution, with a phase A being an equilibrium buffer solution composed of 40-60 mM of Tris-HCl, 3-8% of glycerol and 0.5-5 mM of DTT, and a phase B being an elution buffer solution composed of 40-60 mM of Tris-HCl, 400-600 mM of NaCl, 3-8% of glycerol and 0.5-5 mM of DTT, the volume ratio of the elution buffer solution being increased from 0 to 20%, the volume ratio of the elution buffer solution being increased from 0 to 20%, the volume ratio of the elution buffer solution being increased from 0 to 20%, and the volume ratio of the elution buffer solution being increased from 0 to 20%; the volume ratio of the elution buffer solution is increased from 20% to 100%, elution is performed by 5-10 times of the column volume, then the volume ratio of the elution buffer solution is increased from 20% to 100%, and elution is performed by 3-6 times of the column volume, so that bromelain and a component with a peptide fragment mixture as a main component are obtained through separation and purification. The method provides a new purification process for producing the bromelain hydrolase, and the peptide fragment mixture obtained by the method has higher activity and a relatively wide application prospect.

The invention provides modified glycosaminoglycans sourced from marine organisms and application of the modified glycosaminoglycans in fresh keeping of chicken. The application of the modified glycosaminoglycan derived from marine organisms in fresh keeping of chicken comprises the following steps: S1, treating chicken, and cutting the treated chicken into blocks for later use; s2, performing enzymolysis on the soft part of the clam by using bromelain, ficin and elastinase to prepare glycosaminoglycan, and then modifying the prepared glycosaminoglycan by using sodium hexametaphosphate to prepare modified glycosaminoglycan from marine organisms; s3, adding the modified glycosaminoglycan derived from the marine organisms into the caesalpinia spinosa gum solution to prepare a fresh-keeping solution loaded with the modified glycosaminoglycan derived from the marine organisms; and S4, soaking the blocky chicken in the fresh-keeping liquid, taking out, draining, carrying out vacuum packaging, and finally, carrying out cold storage. The fresh-keeping liquid prepared by the invention is applied to fresh keeping of chicken, inhibits the formation of ice crystals, obstructs the invasion of oxygen and microorganisms, enhances the antibacterial effect and slows down the fat oxidation rate of chicken.

This invention relates to a method for quantitatively detecting residual DNA and glycosaminoglycan (GAG) levels in cartilage tissue-engineered samples. The method involves enzymatically digesting decellularized cartilage matrix samples using a combination enzyme, wherein the combination enzyme consists of papain and bromelain, with a mass ratio of papain to bromelain of 1-3:1. The digested sample is then centrifuged and ultrafiltered to obtain a test liquid containing DNA and GAG. DNA and GAG detection are then performed on the test liquid containing DNA and GAG, respectively. This invention, through the selection of enzyme types, allows the digested sample to be used for both DNA and GAG detection, improving detection efficiency and reducing the amount of tissue-engineered sample required, thus lowering sample usage costs.

The present invention discloses a quercetin-eucalyptus-oil composition for clearing and protecting the lungs, relating to the technical field of functional foods. The composition comprises active functional components and a three-phase layered targeted-delivery system. The active functional components comprise, in parts by weight, 5-20 parts quercetin, 1-8 parts eucalyptus oil, 2-10 parts bromelain, 10-30 parts dihydromyricetin, 1-6 parts perillaldehyde, 3-15 parts icariin, and 1-5 parts ultra-low-molecular-weight sodium hyaluronate. The three-phase layered targeted-delivery system comprises three independent encapsulation units, namely enteric-protective microspheres encapsulating bromelain, nano mixed micelles encapsulating quercetin, dihydromyricetin, and icariin, and sustained-release microcapsules encapsulating eucalyptus oil and perillaldehyde. By scientifically combining the seven active functional components with the three-phase layered targeted-delivery system and by improving the preparation process, the present invention solves the problems of component incompatibility and easy inactivation in conventional compositions, improves ingredient utilization, enables large-scale production, and enhances the storage stability of the composition.