372 results about "Bromelains" patented technology

A prophylactic treatment for the human malady clinically described as migraine headache comprising daily administration in unit dosage form of a first formulation which comprises a major amount of bioactive peptides and a minor amount of probiotics. Concurrently, daily administration in dosage form of a second formulation of a major amount of active components like malic acid, sylibum marianum, acetyl-L-cysteine, copper chelate, zinc gluconate, aspartate and bromelain. A minor amount of plant derivatives excipients comprise the balance of the second formulation. Preferably, these plant derivatives include beet root, powder, watercress, celery, dandelion, capsicum and artichoke extract.

The invention discloses a manufacture method of recombinant dried beef. The method comprises the following steps: green beef or thawed beef is selected; regarding the beef sample quantity of 0.46mg/g as a reference, bromelain is weighed (specific activity unit: 1200Mu/g ) and diluted into bromelain solution, and then is injected into different parts of the beef; after being rolled and rubbed for 10 to 20 minutes, the beef reacts at the temperature of 40 DEG C for 1.5 hours; the beef is prickled; the prickled beef is dewatered and divided into two parts; one part is steamed and boiled until maturity, and cut and blended as beef grains; the other part of the green beef is dewatered and cut and blended as beef emulsion; the boiled and cut and blended beef grains and the green beef emulsion are evenly mixed; the proportion of the beef grains and the beef emulsion is 0.5-2: 1; TG enzyme is added to the beef by 2-10mg/g; the cooked and the fresh beef are jointed and shaped under the condition that the TG enzyme and the pressure are 1.5-3 N/cm<2>; and then the beef is steamed and boiled, roasted and packaged. The dried beef has the advantages of soft mouth feel, low hardness and bright color and luster.

The present invention provides compositions comprising mixtures of papain and bromelain, combined with a pharmaceutically acceptable carrier, for treating viral-induced skin lesions. The present invention further provides methods of treating viral-induced skin lesions by administering to a subject in need thereof a therapeutically effective amount of papain and bromelain.

The invention discloses a method for preparing fishskin fish-scale collagen protein. The method includes the steps of processing fishskin, processing fish scale, preparing collagen protein and the like. The fish scale processing includes the steps of preprocessing, decalcifying, neutralizing and removing immunities, washing, injecting sol, solid and liquid separation, filtering and obtaining a water-soluble collagen solution generated after fish scale is removed. The collagen protein preparing includes the steps of hydrolysis, enzymolysis, collagen protein filtering and separation, enzyme deactivation, decolorization, fishy smell elimination, drying and the like. The ash content in obtained collagen peptide is obviously lower than that of other enzymolysis methods, and no fishy smell exists. According to the method, hydrolysis is conducted on collagen through compound protease and bromelain, the yield of micromolecule collagen peptide is high, and the enzymic preparation is low in cost and free of bitter taste. By means of the method, the production cost of the collagen peptide can be lowered remarkably, the obtained collagen peptide is white and tasteless powder, the ash content is smaller than 0.5%, and peptide fragment with the molecular weight smaller than 3000 Da accounts for over 96% of total nitrogen.

The invention provides a method for preparing small-molecular weight collagen active peptide through enzymolysis of yak bone and skin. The method comprises the steps of raw material treatment, selective enzymatic hydrolysis of a first enzyme, selective enzymatic hydrolysis of a second enzyme and purifying treatment, wherein the first enzyme is one or more of papain, pepsase, alkaline protease, neutral protease, bromelain and trypsin; and the second enzyme is one or more of papain, pepsase, bromelain and trypsin. In the invention, the collagen peptide is directly extracted and prepared by use of an ultrahigh-pressure compound enzyme; the method has the advantages of simple process, short time, low energy consumption, low cost and greenness and environmental friendliness; and the prepared collagen peptide is invariable and has high yield, small molecular weight and high activity and is easily absorbed and utilized by a human body.

The invention relates to an enzymatic measuring method of glycosylated serum protein and a relative liquid stabilizer, with wide application in medical and biochemical technical field. The invention is characterized in that glycosylated serum protein is digested by bromelain to generate fructose amino acid, fructose amino acid oxidase is reacted with the fructose amino acid to generate H2O2 to be reacted with chromogen DA-64 in the presence of peroxidase to convert DA-64 into one green product, the product content is in direct proportion with glycosylated serum protein content of sample, and an automatic biochemical analyzer uses two-point end point method to measure the content of glycosylated serum protein in the sample. The inventive liquid measuring agent has high stability, easy application, strong interference resistance and non pollution on the pipeline of automatic biochemical analyzer, which is suitable for automatically measuring glycosylated serum protein in batch.

The invention discloses a preparation method of a pure natural meat flavor seasoning. The preparation method comprises the following steps: stewing preprocessed frozen meat at the high temperature, and then centrifuging to obtain meat residue, pulping the meat residue and then carrying out enzymolysis by using bromelain and novozymes flavor protease, storing broth for later use, deactivating enzyme and sterilizing the meat juice after carrying out enzymolysis, centrifuging and removing bone residue, and then mixing the meat juice with the storage broth, then concentrating in vacuum to obtain semi-solid state seasoning, carrying out spray drying, sieving and then packaging. By stewing at the high temperature, the product has the stewing feeling of over-fired and stewed soup; two enzymes are combined for carrying out enzymolysis and a generation quantity of free amino acids is controlled, thus the product is more delicious in taste and richer in flavor; by compounding the broth obtained by stewing at the high temperature with the meat juice obtained by enzymolysis, the width and the thickness of the prepared product are increased; by spray drying, all nutritional ingredients can be perfectly retained, the quality of the product can be improved and the quality guaranteeing period can be prolonged; the prepared product is the pure natural meat flavor seasoning which can be comparable with high-quality products in international similar products.

Compositions containing bromelain are disclosed for use as topical therapeutic agents to restore healthy skin, and for immediate and extended relief from itching and irritation associated with contact dermatitis, insect bites, idiopathic itch, chronic itch, hives, psoriasis, seborrhea, eczema and cracked fingertips, skin abrasions, cuts and minor burns as well as other indications. The compositions include lotions, creams and ointments.

The invention relates to a method for extracting sialic acid from yolk powder. The invention has the following technical characteristics: hydrolyzing yolk powder by a specific bromelain; then removing proteolipin by D type resin; and purifying and extracting sialic acid. Compared with the prior art, the method resolves the pollution problem caused by acid hydrolysis, and overcomes degreasing difficulty and filtering problem of enzymatic hydrolysis of yolk powder. The sialic acid extracted by the method has advantages of simple process, convenient usage, low extraction cost and practical value in industrial production.

A method for inhibiting dipeptidyl-peptidase IV is provided and includes administering a peptide to the dipeptidyl-peptidase IV, in which the peptide is isolated from a gelatin hydrolysate produced by enzymatic digestion with alcalase (ALA), bromelain (BRO), and flavourzyme (FLA).

The invention discloses application of zinc sulfate in the aspect of inactivating bromelain at room temperature. The method comprises the following steps: preparing a bromelain solution, diluting the taken 1g of bromelain (food grade, specific activity unit: 50 ten thousand U/g) for 100 times, namely, firstly performing constant volume to 100ml, performing the constant volume on 5ml of bromelain solution to 50 ml; and adding zinc sulfate: respectively adding 0.1-4mg (zinc sulfate)/mL (bromelain solution), wherein 0.02mg of bromelain is contained in each mL of bromelain solution. According to the invention, the inactivation effect is good and safety is high, and the proper softness of a meet product can be ensured by using the zinc sulfate in the meat product.

The invention provides a method for synchronously extracting grease and antioxidative peptide from soybeans and belongs to the food processing technology. The method comprises steps as follows: (1), the soybeans are smashed and sieved, water is added for soaking, and a semi-solid mixed solution is formed; (2), the semi-solid mixed solution is pretreated with a high-voltage pulse electric field; (3), three-enzyme compounds of Protex-7L neutral protease, alkaline protease and bromelain are added to the mixed solution after being subjected to pulse electric field treatment for enzymolysis; (4), the mixed solution after enzymolysis is cooled to 4 DEG C, and soybean grease and hydrolysate are obtained through centrifugation and separation; (5), the hydrolysate is taken and subjected to closure separation by a 10kDa ultrafiltration membrane (a PP-100 hollow-fiber module), and a soybean peptide solution with molecular weight of 10 kDa or lower is obtained; (6), the soybean peptide solution is subjected to vacuum freeze drying, and the antioxidative soybean peptide with high added value can be directly obtained.

The invention relates to peptide coffee using coffee beans as the raw materials, and a preparation method of the peptide coffee. The peptide coffee contains 1 to 8% of peptide which can be absorbed by a human body, and also contains linoleic acid, linolenic acid, oleic acid, caffeine, VB, calcium, ferrum, phosphorus, selenium and glucose. The preparation method comprises the steps of baking coffee beans under a temperature of 80 to 150 DEG C; crushing; feeding water; pulping and grinding until the fineness is of 200 to 300 meshes; homogenizing at two to four times under a high pressure of 40 to 60Mpa; grinding at the temperature of 135 to 160 DEG C and under the pressure of 7 to 8kG until being up to more than 300 meshes; heating for 35 to 40 minutes; cooling to reach the temperature of 30 to 55 DEG C; feeding hydrochloric acid and then feeding lipase and cellulose; standing and subliming; then conditioning the temperature to reach 45 to 50 DEG C; feeding bromelain to sublime under a constant temperature again; heating the sublimed pulp to reach the temperature of 80 to 100 DEG C; performing enzyme deactivation and sterilization; enabling residual hydrochloric acid to volatilize to obtain peptide coffee stoste; then drying to obtain the peptide coffee.

The invention relates to stomatology and perfume industry in particular to therapeutic formulation for oral cavity care. The invention refers to oral hygiene means and can be applied for preparation of medical and prophylactic tooth-pastes, gels, liquids for oral care as well as other compositions like chewing gums, jellies, etc. Formulation containing orally applicable active and inert components involves bromelain in amount of 0.01÷1 wt %, xylitol 1.5÷20 wt %. At best bromelain content is 0.1—0.8 wt %, xylitol content—2.2-18 wt %. For attaining remineralizing effect formulation contains anti-carious mineral additive 0.05-3.0 wt % of the list of active components. At best anti-carious mineral additive content is 0.2-2.5 wt %. There are used calcium glycerophosphate and inorganic or organic magnesium salt as anti-carious mineral additive. It is acceptable to use sodium monofluorphosphate, potassium monofluorphosphate, calcium monofluorphosphate or magnesium monofluorphosphate in amount of 0.5-1.5 wt % as anti-carious mineral additive Optimum concentration is 0.8-1.1 wt %.

The invention discloses a preparation method for sea cucumber peptide, belonging to the technical field of aquatic product processing. The preparation method comprises the following steps: weighing cooked fresh sea cucumber without internal organs, immersing the sea cucumber in liquid nitrogen, taking the sea cucumber out, then soaking the sea cucumber in hot water with a temperature of 95-97 DEGC, and then carrying out stirring and washing; adding the cleaned sea cucumber into a cooking and enzymolysis tank for cooking, and after completion of cooking, starting the stirrer and the shearer ofthe cooking and enzymolysis tank and performing shearing to obtain slurry; filtering the slurry, then pumping the filtered slurry into the cooking and enzymolysis tank, adding composite neutral protease consisting of thiol protease-series bromelain, papain and Bacillus subtilis-produced neutral protease, and carrying out enzymolysis under a heat-preserved condition; and subjecting an enzymolysisproduct to post-treatment to obtain a finished sea cucumber peptide product. With the method in the invention, harmful heavy metals in a sea cucumber raw material can be completely removed; small-molecular peptide with a molecular weight of 1000 D or below in the enzymolysis product accounts for 85% or more; and the finished product has no fishy and peculiar smell.

The invention relates to a complex enzyme preparation for sewage treatment. The complex enzyme preparation is compounded by mixing complex enzyme and mineral water, wherein the weight ratio of the complex enzyme to the mineral water is 1:9-50; and the complex enzyme contains the following components in volume content: 50 to 80 mg/L of bromelain, 50 to 80 mg/L of papain, 15 to 30 mg/L of L-aspartase, 15 to 30 mg/L of glutathione, 100 to 150 mg/L of yeast extract and 50 to 80 mg/L of cellulose. The complex enzyme preparation improves the treatment capability of a sewage plant, reduces the running cost, basically achieves zero release of residual organic sludge, prevents environmental secondary pollution of the sewage treatment, improves the self-purification capability of yielding water of the sewage plant, prevents secondary pollution of the yielding water, prevents secondary eutrophication of the yielding water, and radically solves the problem of environmental protection in operation of the sewage treatment plant.

The invention relates to a method for preparing bromelain, and in particular relates to a method for extracting high-purity pharmaceutical grade bromelain. The method comprises the following steps of: pre-treating pineapple; performing coarse crushing and zymolysis; squeezing a juice; filtering; adding an auxiliary material; performing fine filtration; adding an stabilizing agent; absorbing by using an absorbing agent; and performing elution, ultrafiltration and freeze-drying to obtain a finished product. The method has the advantages that a kaolin adsorption method, a tannin precipitation method and an organic solvent extraction method in the conventional extraction of bromelain are replaced by a cellulose adsorption method, so that the yield is high, the process is simple, the energy consumption is low, and the indexes including activity, bacterial content, heavy metal content, appearance and the like of the produced bromelain reach or exceed a pharmaceutical grade standard. Besides, adsorptive cellulose can be used repeatedly after elution and regeneration, so that the production cost can be greatly reduced.

The invention relates to an acne-removing composition and a preparation method thereof. The acne-removing composition is characterized by comprising 1%-20% by mass of acne-removing active matter; the acne-removing active matter is composed of 10-15 parts of zinc pyrrolidone carboxylic acid, 4-6 parts of sophorolipid, 5-8 parts of bromelain, 8-12 parts of amur cork tree bark extract, 8-16 parts of purple coneflower extract, 12-18 parts of lithospermum extract, 3-8 parts of humic acid and 40-50 parts of isomalt. The acne-removing composition has the advantages of being excellent in acne-removing effect, safe, stable and convenient to prepare.

The present invention provides a pet deodorizing tablet which is mainly prepared by using the following components in parts by weight: complex enzymes 8-21 parts, yucca saponin 5-10 parts, green tea extract 20-30 parts, probiotics 8-28 parts, dextrin 5-30 parts, aspartame 1-3 parts, starch 5-15 parts, magnesium stearate 0.5-2 parts, and the complex enzymes comprise the following components: papain 1500-2000 u/g, bromelain 3000-4000 u/g, phytase 1000-1680 u/g, cellulase 600-750 u/g, beta-glucanase 1500-2300 u/g and trypsin 1800-2560 u/g. The pet deodorizing tablets can comprehensively eliminate pets' bad breath, shit smell and other body odors, have a more comprehensive and effective deodorizing effect, and can effectively improve the gastrointestinal function, immunity and health condition of pets.

The invention mainly relates to the technical field of silk processing, and discloses an environment-friendly silk degumming method, which includes immersion, enzymolysis, fermentation and washing. The method is simple, conditions are mild, high temperature and high pressure are not needed, energy is saved, the method is suitable for batch processing, the degumming rate can reach 89.3 percent, obtain silk is fine and smooth, the color is bright and white-yellow, and economic income can be increased by 12.7 percent. pH of immersed silk is regulated to be alkaline, lipase is added to decompose grease and wax components in the silk, high-temperature cooking is avoided, so that energy is saved, pH is then regulated to be acidic, bromelain is added, and enzymic preparations with different pHs are sequentially used for enzymolysis. The conventional joint use of alkaline protease and surfactant is changed, the environment is protected, soluble impurities under different pHs are sufficiently removed, sericin and remaining fat are decomposed, and the silk degumming rate is increased.

A composition to relieve itch, pain and swelling associated with a bite or sting, comprising a proteolytic enzyme dissolved in water, an anti-bacterial agent, and a pH lowering agent. The composition is in a dispensing container, and is devoid of other ingredients that may mask the effectiveness of the enzyme. In one form a liquid propellant floats on the composition and a dip tube extends from a discharge valve into the composition. In another form, a piston separates the composition from a pressurized gaseous propellant. In another form, the composition is in a bag in the container and pressurized gaseous propellant surrounds the bag. In a further form the bag is an elastic material that exerts pressure on the composition. In other forms the container is a single use bladder or pouch. The proteolytic enzyme preferably is a meat tenderizer such as Papain or Bromelain.