18 results about "Bromelains" patented technology

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention discloses modified hydroxytyrosol as well as a preparation method, cosmetics and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, hydroxytyrosol and bromelain are mixed for an enzymatic hydrolysis reaction, and the modified hydroxytyrosol is prepared. According to the provided preparation method of the modified hydroxytyrosol, the hydroxytyrosol is subjected to catalytic modification through bromelain, bromelain serves as typical sulfydryl protease and is subjected to an enzymolysis reaction with the hydroxytyrosol, the hydroxytyrosol enzymolysis derivative is generated, and the generated hydroxytyrosol enzymolysis derivative contains various substances with aromatic smells, so that the hydroxytyrosol can be used for preparing the modified hydroxytyrosol. The obvious pungent smell of the hydroxytyrosol raw material is effectively improved.

The present application relates to a kind of methods for preparing high-purity ecdysone from dew grass, comprising (1) pretreatment, (2) enzymolysis, the complex enzyme includes cellulase, pectinase, papain and bromelain;(3) extraction, (4) purification: dew grass extract is passed through macroporous adsorption resin, sequentially using alkali washing, one water washing, acid washing, twice water washing, ethanol elution, concentration;(5) bleaching, (6) crude crystallization, (7) recrystallization.The purity and yield of β-ecdysone product obtained by the method of the present application are high, and the composite enzyme enzymolysis is combined with ultrasonic countercurrent extraction, which can efficiently and quickly extract;During the purification process, low-concentration alkali washing and acid washing can effectively elute impurities, improve the purity of β-ecdysone, and also reduce the difficulty of macroporous resin regeneration, improve production efficiency.The method of the present application is simple, and the resin and solvent used can be reused, with low process cost, suitable for industrial production.

The invention relates to a preparation method of a rhodomyrtus tomentosa fruit extract as well as a product and application of the rhodomyrtus tomentosa fruit extract. The preparation method comprises the following steps: mixing a rhodomyrtus tomentosa fruit raw material with a compound enzyme for enzymolysis, adding an ethanol aqueous solution into an enzymolysis reaction solution after enzymolysis is completed, performing negative pressure cavitation extraction, filtering and collecting filtrate to obtain the rhodomyrtus tomentosa fruit extract, the compound enzyme comprises a combination of cellulase, bromelain, alpha-amylase and tannase. According to the method, the effective components in the longan pulp are extracted by adopting the specific compound enzyme, the related components in the rhodomyrtus tomentosa fruits can be efficiently extracted, a potential synergistic relationship exists among bromelain, alpha-amylase and tannase, the extraction effect can be remarkably improved after the bromelain, the alpha-amylase and the tannase are combined, and the method is suitable for industrial production. The prepared extract product has the effects of resisting inflammation, relieving, resisting oxidation and the like.

The present application relates to a kind of preparation method of immunoglobulin powder and its application, the preparation method includes the following steps: collecting bovine colostrum, using cell survival technology is frozen;Frozen bovine colostrum is thawed, carries out milk fat separation, and obtains defatted bovine colostrum;Defatted bovine colostrum and composite rennet are mixed and are curdled, and then broken, obtain whey;The composite rennet includes aspartic protease, papain and bromelain;Whey is ultrafiltrated, and immunoglobulin solution is obtained;Immunoglobulin solution is vacuum freeze-dried, and the immunoglobulin powder is obtained.The preparation method provided by the present application is low temperature all the way, the activity of immunoglobulin is retained to the greatest extent, the use of composite rennet solves the problem of curd failure, and each link cooperates to improve the curd effect, realizes the preparation of high-efficiency, high-purity and high-quality immunoglobulin powder.

A method for treating and / or improving inflammatory intestinal disease includes administering a composition to a patient suffering from inflammatory intestinal disease to be capable of effectively inhibiting the patient's intestinal bleeding, intestinal inflammation, and capable of achieving efficacies of repairing intestinal cells and intestinal wall structure. The composition contains an effective amount of bromelain and / or an effective amount of Bidens pilosa extract.

The invention belongs to the technical field of livestock and poultry by-product processing, and particularly relates to an efficient tenderization method for livestock and poultry by-products. The method comprises the following steps: preparing a papain solution and a bromelain solution by taking natural small molecule water as a solvent, and tenderizing the livestock and poultry byproducts by adopting the papain solution and the bromelain solution. Natural small molecule water is used for dissolving papain and bromelain for directional efficient tenderization, the problem of processing quality of livestock and poultry byproducts can be solved, and uniformity of internal and external taste of the byproducts is effectively achieved.

The invention discloses a whey protein peptide with high anticoagulant activity and a blood fat reducing effect and a preparation method thereof. The preparation method comprises the following steps: firstly carrying out pulsed electric field pretreatment on a whey protein aqueous solution, then hydrolyzing the pretreated whey protein aqueous solution by using bromelain, and freeze-drying to obtain the whey protein peptide. According to the method, bromelain is selected to carry out enzymolysis on the whey protein aqueous solution, and the whey protein aqueous solution is pretreated by using the pulsed electric field before enzymolysis, so that the use of the pulsed electric field is beneficial to promoting the exposure of peptide chain segments with anticoagulation and blood fat reduction functions, and the anticoagulation and blood fat reduction capabilities of the whey protein peptide are remarkably improved. The method breaks through the limitation of the traditional process, and overcomes the problems of low efficiency and limited active peptide release of a single enzymolysis method through the synergistic effect of the pulsed electric field pretreatment and the enzymolysis technology. The non-thermal effect of the pulsed electric field can directionally change the space structure of the whey protein, more enzyme cutting sites are exposed, and meanwhile protein denaturation caused by high-temperature treatment is avoided.

The application discloses a quercetin-eucalyptus oil composition for clearing and protecting lungs, and relates to the technical field of functional food. The composition comprises an active functional component and a three-phase layered targeting delivery system. The active functional component is composed of quercetin 5-20 parts, eucalyptus oil 1-8 parts, bromelain 2-10 parts, dihydromyricetin 10-30 parts, perillaldehyde 1-6 parts, icariin 3-15 parts and ultralow-molecular-weight sodium hyaluronate 1-5 parts in terms of weight fraction. The three-phase layered targeting delivery system comprises three independent embedding units, namely enteric-soluble protective microspheres for wrapping bromelain, nano-mixed micelles for wrapping quercetin, dihydromyricetin and icariin, and slow-release microcapsules for wrapping eucalyptus oil and perillaldehyde. The application solves the problems of component compatibility conflict and component inactivation in traditional compositions by scientifically matching seven active functional components and combining the three-phase layered targeting delivery system, improves the utilization rate of components, can be produced on a large scale, and improves the storage stability of the composition.

The invention discloses a preparation method of highland barley protein peptide with hypoglycemic activity, and belongs to the field of biotechnology and functional food. According to the preparation method, highland barley protein is extracted from highland barley powder by adopting an alkali-solution and acid-isolation method, then the highland barley protein peptide is prepared by sequentially adopting alkaline protease and bromelain to carry out step-by-step enzymolysis, and the complementary and synergistic effects of different restriction enzyme cutting sites are realized through a step-by-step enzymolysis strategy of'alkaline protease-bromelain 'in a specific sequence. Aromatic and hydrophobic amino acids are preferentially cut by the alkaline protease, the space structure of the protein is opened, and more effective sites are provided for bromelain, so that small molecular peptide fragments with hypoglycemic activity are more efficiently released, and in-vitro experiments show that the inhibition rate of the bromelain on alpha-amylase is up to 52.49%, and the inhibition rate of the bromelain on the bromelain is up to 52.49%. The inhibition rate of alpha-glucosidase reaches up to 73.35%, which is far better than that of a single enzymolysis product.

The present invention discloses a quercetin-eucalyptus-oil composition for clearing and protecting the lungs, relating to the technical field of functional foods. The composition comprises active functional components and a three-phase layered targeted-delivery system. The active functional components comprise, in parts by weight, 5-20 parts quercetin, 1-8 parts eucalyptus oil, 2-10 parts bromelain, 10-30 parts dihydromyricetin, 1-6 parts perillaldehyde, 3-15 parts icariin, and 1-5 parts ultra-low-molecular-weight sodium hyaluronate. The three-phase layered targeted-delivery system comprises three independent encapsulation units, namely enteric-protective microspheres encapsulating bromelain, nano mixed micelles encapsulating quercetin, dihydromyricetin, and icariin, and sustained-release microcapsules encapsulating eucalyptus oil and perillaldehyde. By scientifically combining the seven active functional components with the three-phase layered targeted-delivery system and by improving the preparation process, the present invention solves the problems of component incompatibility and easy inactivation in conventional compositions, improves ingredient utilization, enables large-scale production, and enhances the storage stability of the composition.

The present application relates to a kind of skin regeneration repair medicine and preparation method thereof, belong to biological medicine technical field, and medicine includes oil palm extraction peptide, tiger palm silver ear extract, Gangdeng root-milletia nitida-Siberian milkvetch composite extract and pomegranate peel polyphenol etc.Oil palm extraction peptide is oil palm sequentially by cellulase, alpha-chymotrypsin and ficin enzymolysis, then by G-50 dextran gel column purification and obtain the product above 1kDa;Tiger palm silver ear extract is tiger palm silver ear fruit body by cellulase and bromelain common enzymolysis, and obtain the product below 3kDa;Gangdeng root-milletia nitida-Siberian milkvetch composite extract is Gangdeng root, milletia nitida and siberian milkvetch by gradient extraction of ethanol aqueous solution and obtain extract, sequentially by polyamide resin column adsorption and LH-60 dextran gel column purification and obtain the product.Multiple components construct good repair system, accelerate wound repair healing.

The invention relates to the technical field of food production, and discloses a production process for pickling chafing dish goose intestines with compound seasonings, which comprises the following steps: S1, microorganism synergistic fermentation pretreatment: soaking goose intestines in a low-salt solution containing lactic acid bacteria and salt, fermenting at 4-6 DEG C, washing with cold water after fermentation is completed, and draining; s2, biological enzyme pretreatment: soaking the goose intestines subjected to fermentation pretreatment in a bromelain solution, treating at 4-6 DEG C, washing with cold water after treatment is completed, and draining; s3, pulse ultrasonic assisted pickling; s4, performing vacuum sealing and low-temperature curing; and S5, closed-loop pickling material recovery. The method comprises the following steps: carrying out microbial synergistic fermentation pretreatment, fermenting for 1.5-2 hours by utilizing a low-salt solution of 1-2% v / v lactic acid bacteria and 2-3 parts by weight of salt, and adjusting the pH of the surface of the goose intestine to 6.0-6.5, so that the permeability of the seasoning is enhanced, the initial microbial load is reduced, and the food safety and flavor uniformity are ensured.

The present application relates to the technical field of in vitro detection, in particular to a rapid sperm morphology staining reagent and a staining method thereof; the rapid sperm morphology staining reagent of the present application can make semen spread rapidly and uniformly after 5 minutes by using bromelain and L-cysteine in combination. The staining reagent of the present application greatly shortens the pretreatment time of the sample and improves the detection efficiency.

This invention discloses a biotechnology-based process and product for preparing deodorized mushroom concentrate, relating to the field of mushroom concentrate technology. The invention involves taking finely filtered mushroom broth, concentrating it, and controlling the solid content to obtain a mushroom concentrate. The mushroom concentrate is then adjusted to pH using sodium hydroxide or hydrochloric acid, heated, and immobilized bromelain and papain are added, stirred, and then immobilized flavor protease is added, stirred, and filtered to obtain the deodorized mushroom concentrate. Bromelain, papain, and flavor protease are selected. Bromelain and papain are endopeptidases that cleave long protein chains in mushrooms at different sites, providing more ends for the flavor protease. The flavor protease is an exopeptidase that hydrolyzes the protein fragments cleaved by bromelain and papain to form flavor amino acids. During the enzymatic hydrolysis process, the immobilized carriers of the three enzymes remove off-odors through adsorption.

The application relates to the technical field of biological separation engineering, and discloses a recombinant biomimetic affinity ligand for purifying bromelain as well as a preparation method and application of the recombinant biomimetic affinity ligand, which is prepared from the following raw materials in parts by weight: double-bonded silica-coated magnetic microspheres: 100 parts; monomer methyl acrylate: 150-250 parts; epoxy-containing monomer glycidyl methacrylate: 50-100 parts; crosslinking agent ethylene glycol dimethacrylate: 3-6 parts; and biomimetic affinity heptapeptide: 10-20 parts. The biomimetic affinity heptapeptide with a specific amino acid sequence is used as the affinity ligand, the hydrophobic residues and charged side chains in the sequence can specifically bind to the S2 sub-site and surrounding residues of the active center of bromelain to realize cavity complementation, high-specificity recognition of the target enzyme is realized, and the affinity medium prepared by using the ligand can effectively eliminate the interference of homologous impure proteins which are highly similar in structure and physicochemical properties.

This invention relates to a method for quantitatively detecting residual DNA and glycosaminoglycan (GAG) levels in cartilage tissue-engineered samples. The method involves enzymatically digesting decellularized cartilage matrix samples using a combination enzyme, wherein the combination enzyme consists of papain and bromelain, with a mass ratio of papain to bromelain of 1-3:1. The digested sample is then centrifuged and ultrafiltered to obtain a test liquid containing DNA and GAG. DNA and GAG detection are then performed on the test liquid containing DNA and GAG, respectively. This invention, through the selection of enzyme types, allows the digested sample to be used for both DNA and GAG detection, improving detection efficiency and reducing the amount of tissue-engineered sample required, thus lowering sample usage costs.

The present invention discloses a quercetin-eucalyptus-oil composition for clearing and protecting the lungs, relating to the technical field of functional foods. The composition comprises active functional components and a three-phase layered targeted-delivery system. The active functional components comprise, in parts by weight, 5-20 parts quercetin, 1-8 parts eucalyptus oil, 2-10 parts bromelain, 10-30 parts dihydromyricetin, 1-6 parts perillaldehyde, 3-15 parts icariin, and 1-5 parts ultra-low-molecular-weight sodium hyaluronate. The three-phase layered targeted-delivery system comprises three independent encapsulation units, namely enteric-protective microspheres encapsulating bromelain, nano mixed micelles encapsulating quercetin, dihydromyricetin, and icariin, and sustained-release microcapsules encapsulating eucalyptus oil and perillaldehyde. By scientifically combining the seven active functional components with the three-phase layered targeted-delivery system and by improving the preparation process, the present invention solves the problems of component incompatibility and easy inactivation in conventional compositions, improves ingredient utilization, enables large-scale production, and enhances the storage stability of the composition.