Method for extracting sialic acid from yolk powder

A technology of egg yolk powder and bird's nest, applied in fermentation and other directions, can solve the problems of difficult degreasing, low product yield, high hydrolysis temperature, etc., and achieve the effect of no environmental pollution, high product content, and simple process

Inactive Publication Date: 2013-04-24
邹如
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In these methods, there are many difficulties in production that cannot be solved, such as the difficulty of degreasing, and some processes require a large amount of organic solvents, which will pollute the product and have a damaging impact on...

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  • Method for extracting sialic acid from yolk powder

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example 1

[0008] Example 1. Take 100kg of egg yolk powder, soak it in 650kg of deionized water, stir for 30min at a temperature of 25°C, add 1kg of bromelain, stir for 10min, control the temperature at 27°C, leave it for 15hr, add sodium alginate with a molecular weight of 100,000 and 15% after stirring 100kg, stir evenly, let it stand for 30min, filter the precipitate with a centrifuge, take the filtrate, add 6% acetic acid to adjust the pH to 4, pass through a macroporous resin column (250kg), collect the eluate, and put it in the perhydrogenated cationic ion exchange Column (100kg), collect the acidic eluate, pass through the ammonium type cation exchange column (200kg), gradient elution, collect the PH3 eluate, adjust the pH7 with 10% sodium carbonate. Centrifuge and filter, place at -5°C for 12 hours, remove the precipitate, concentrate and crystallize, and dry to obtain the bird's nest acid product with a yield of 22.5%. If the product is dark in color, it can be decolorized with ...

example 2

[0009] Example 2. Take 200kg of egg yolk powder, soak it in 1500kg of deionized water, stir for 30min at a temperature of 25°C, add 0.7kg of bromelain, stir for 15min, control the temperature at 25°C, leave it for 18hr, add alginic acid with a molecular weight of 150,000 and 15% after stirring Sodium 200kg, stir evenly, let it stand for 30min, filter the precipitate with a centrifuge, take the filtrate, add 8% acetic acid to adjust the pH to 4, pass through a macroporous resin column (300kg), collect the eluate, and put Exchange column (200kg), collect acidic eluent, ammonium type cation exchange column (300kg), gradient elution, collect PH4 eluate, adjust pH7 with 15% sodium carbonate. Centrifuge and filter, place at -5°C for 15 hours, remove the precipitate, concentrate and crystallize, and dry to obtain the bird's nest acid product. Yield 27%.

example 3

[0010] Example 3. Quality inspection

[0011] 1.2 Method

[0012] 1.2.1 Chromatographic conditions Chromatographic column: Symmetry C18 column (4.6mm×250mm, 5μml); column temperature: 35°C; mobile phase: 1.0% tetrahydrofuran aqueous solution (containing 0.2% phosphoric acid)-acetonitrile (92:8); detection wavelength: 230nm; flow rate: 1.0ml / min; injection volume: 10μl

[0013] 1.2.2 Preparation of sample solution Accurately weigh 1.0mg of bird’s nest acid product, dissolve it in 100ml of water for injection, then add 1ml of 10mg / ml phthalimine hydrochloride solution, heat in 80°C water bath for 40min, and set aside.

[0014] 1.2.3 Preparation of reference substance solution Take 2ml of N-acetylneuraminic acid (sialic acid) reference substance solution, then add 1ml of 10mg / ml o-phenylenediamine hydrochloride solution, heat in 80°C water bath for 40min, take it out After cooling, after filtering through a 0.45 μm membrane, 10 μl each of the above solution and the reference so...

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Abstract

The invention relates to a method for extracting sialic acid from yolk powder. The invention has the following technical characteristics: hydrolyzing yolk powder by a specific bromelain; then removing proteolipin by D type resin; and purifying and extracting sialic acid. Compared with the prior art, the method resolves the pollution problem caused by acid hydrolysis, and overcomes degreasing difficulty and filtering problem of enzymatic hydrolysis of yolk powder. The sialic acid extracted by the method has advantages of simple process, convenient usage, low extraction cost and practical value in industrial production.

Description

technical field [0001] The invention relates to the extraction of biological products and its application Background technique [0002] Sialic acid, also known as sialic acid, is a kind of neuraminic acid, which is an important component of cell protein and widely exists in animal brain tissue cells. Recent studies believe that sialic acid can prevent Alzheimer's disease and improve immunity. A powerful neurotrophic product, sialic acid connects to the terminal tissue of cell protein glycolipids, improves the recognition ability of brain cells, promotes the absorption of minerals and vitamins in the intestine, and has a significant inhibitory effect on early cancer and metastasis. The medicinal prospect of sialic acid is very broad, and it is also a key project in the field of biopharmaceuticals in recent years. [0003] The extraction process of bird's nest acid has been reported in literature, and it is extracted from whey and egg yolk powder, such as US 5233033, US 52704...

Claims

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Application Information

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IPC IPC(8): C12P19/26
Inventor 邹如
Owner 邹如
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