Method for extracting high-purity pharmaceutical grade bromelain

A technology of bromelain and extraction method, which is applied in the field of extraction of high-purity pharmaceutical-grade bromelain, can solve the problems of high cost, complex extraction process, enzyme bacteria content, and heavy metal content that cannot meet the pharmaceutical-grade standard, and achieve energy consumption reduction, The effect of high recovery rate of enzyme activity and shortening of drying time

Active Publication Date: 2013-09-18
四川杨天生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the disadvantages of complex extraction process and high cost in the prior art, and the bacteria content and heavy metal content of the produced enzyme cannot reach the pharmaceutical gra

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The extraction method of high-purity pharmaceutical-grade bromelain in this embodiment includes the following steps: pretreatment of pineapple raw materials, coarse crushing, enzymolysis, juice extraction, filtration, addition of auxiliary materials, fine filtration, addition of stabilizers, adsorption by adsorbents, and elution , ultrafiltration, and freeze-drying to obtain the finished product.

[0039] Specifically: after cleaning the raw materials, put them in a 4°C environment for precooling for 24 hours, and then crush them with a hammer mill; add ligninase with a mass percentage of 0.001% to the crushed pineapple pieces and mix them evenly, and then transport them to the press through a conveyor belt. There is a receiving tank under the squeezer to collect pineapple pulp; the pineapple pulp is coarsely filtered through a bag filter, and then circulated through a horizontal filter to obtain pineapple juice with fine residue; Add the total mass of 0.2% sodium benzo...

Embodiment 2

[0041] The extraction method of high-purity pharmaceutical-grade bromelain in this embodiment includes the following steps: pretreatment of pineapple raw materials, coarse crushing, enzymolysis, juice extraction, filtration, addition of auxiliary materials, fine filtration, addition of stabilizers, adsorption by adsorbents, and elution , ultrafiltration, and freeze-drying to obtain the finished product.

[0042] Specifically: after cleaning the raw materials, place them in an environment of 2°C for precooling for 20 hours, and then crush them with a hammer mill; add 0.003% ligninase by mass percentage to the crushed pineapple pieces and mix them evenly, and then transport them to the press through a conveyor belt. There is a receiving tank under the press machine to collect pineapple pulp; the pineapple pulp is coarsely filtered through a bag filter, and then circulated through a horizontal filter to obtain pineapple juice with fine residue; Add the total mass of 0.5% sodium b...

Embodiment 3

[0044]The extraction method of high-purity pharmaceutical-grade bromelain in this embodiment includes the following steps: pretreatment of pineapple raw materials, coarse crushing, enzymolysis, juice extraction, filtration, addition of auxiliary materials, fine filtration, addition of stabilizers, adsorption by adsorbents, and elution , ultrafiltration, and freeze-drying to obtain the finished product.

[0045] Specifically: after cleaning the raw materials, put them in a 5°C environment for precooling for 30 hours, and then crush them with a hammer mill; add ligninase with a mass percentage of 0.01% to the crushed pineapple pieces and mix them evenly, and then transport them to the press through a conveyor belt. There is a receiving tank under the squeezer to collect pineapple pulp; the pineapple pulp is coarsely filtered through a bag filter, and then circulated through a horizontal filter to obtain pineapple juice with fine residue; Add the total mass of 0.1% sodium benzoat...

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Abstract

The invention relates to a method for preparing bromelain, and in particular relates to a method for extracting high-purity pharmaceutical grade bromelain. The method comprises the following steps of: pre-treating pineapple; performing coarse crushing and zymolysis; squeezing a juice; filtering; adding an auxiliary material; performing fine filtration; adding an stabilizing agent; absorbing by using an absorbing agent; and performing elution, ultrafiltration and freeze-drying to obtain a finished product. The method has the advantages that a kaolin adsorption method, a tannin precipitation method and an organic solvent extraction method in the conventional extraction of bromelain are replaced by a cellulose adsorption method, so that the yield is high, the process is simple, the energy consumption is low, and the indexes including activity, bacterial content, heavy metal content, appearance and the like of the produced bromelain reach or exceed a pharmaceutical grade standard. Besides, adsorptive cellulose can be used repeatedly after elution and regeneration, so that the production cost can be greatly reduced.

Description

technical field [0001] The invention relates to a method for extracting bromelain, in particular to a method for extracting high-purity pharmaceutical grade bromelain. Background technique [0002] Bromelain is a proteolytic enzyme. Bromelain extracted from pineapple stems is a pure natural plant protease that has a strong ability to decompose fibrin and blood clots. At the same time, bromelain has anti-inflammatory, enhances drug absorption, Prevention and treatment of cardiovascular diseases, inhibition of tumor cell growth, etc., are widely used in the pharmaceutical industry. [0003] At present, the extraction methods of bromelain mainly include chemical extraction and physical extraction methods. For example, the patent CN1908165A provides a new method for physical extraction of bromelain. The process steps for extracting bromelain are: raw materials-cleaning-crushing-pretreatment- Juicing-centrifugation-freezing-filtration-ultrafiltration-drying-finished product, the...

Claims

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Application Information

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IPC IPC(8): C12N9/50
Inventor 诸辉石亚妮吴吕平
Owner 四川杨天生物药业股份有限公司
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