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Method for extracting and obtaining bromelain from pineapple peel residues

A technology of bromelain and pineapple peel residues, applied in the direction of sterilization methods, methods of supporting/immobilizing microorganisms, biochemical equipment and methods, etc., can solve waste of raw materials, can not effectively improve enzyme purity and enzyme activity, pineapple peel Solve the problems of low utilization rate of slag and enzyme yield, and achieve the effect of improving utilization rate, improving utilization rate and enzyme yield, and high juice extraction rate

Inactive Publication Date: 2019-06-14
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the existing bromelain extraction process, pineapple stems are usually used for preparation, and pineapple pomace is not used, which wastes raw materials. Purity and enzyme activity, so a method for extracting bromelain from pineapple pomace was designed

Method used

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  • Method for extracting and obtaining bromelain from pineapple peel residues
  • Method for extracting and obtaining bromelain from pineapple peel residues
  • Method for extracting and obtaining bromelain from pineapple peel residues

Examples

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Embodiment 1

[0033] A method for extracting and obtaining bromelain from pineapple pomace, comprising the following steps:

[0034] Step 1. Wash the pineapple skin residue, first put it into a crushing and filtering device for multi-stage crushing, then add 0.1% ascorbic acid, and add 1Kg of distilled water, and finally obtain the filtrate by squeezing and filtering;

[0035] Step 2, add 0.05% calcium propionate to the above filtrate for anticorrosion, stir and centrifuge the filtrate at a high speed for 15 minutes at a stirring speed of 5000r / min, take the supernatant, and add pre-dissolved tannin solution to the supernatant, while Stir while adding, so that the final concentration of tannin is 0.2%. After adding tannin, let it stand still, and after the formed tannin-enzyme complex is precipitated, carefully remove the supernatant by siphon method to obtain the lower compound The concentrated solution of the compound; the concentrated solution of the lower complex is separated and washed...

Embodiment 2

[0041] A method for extracting and obtaining bromelain from pineapple pomace, comprising the following steps:

[0042] Step 1. Wash the pineapple skin residue, first put it into a crushing and filtering device for multi-stage crushing, then add 0.05% ascorbic acid, and add 0.5Kg distilled water, and finally obtain the filtrate by squeezing and filtering;

[0043] Step 2, add 0.03% calcium propionate to the above filtrate for anticorrosion, stir and centrifuge the filtrate at a high speed for 10 minutes at a stirring speed of 4000r / min, take the supernatant, and add pre-dissolved tannin solution to the supernatant, while Stir while adding, so that the final concentration of tannin is 0.1%. After adding tannin, let it stand still, and after the formed tannin-enzyme complex is precipitated, carefully remove the supernatant by siphon method to obtain the lower layer compound The concentrated solution of the compound; the concentrated solution of the lower complex is separated and ...

Embodiment 3

[0048] Step 1. Wash the pineapple skin residue, first put it into a crushing and filtering device for multi-stage crushing, then add 0.1% ascorbic acid, and add 0.5Kg distilled water, and finally obtain the filtrate by squeezing and filtering;

[0049] Step 2, add 0.05% calcium propionate to the above filtrate for anticorrosion, stir and centrifuge the filtrate at a high speed for 10 minutes at a stirring speed of 5000r / min, take the supernatant, and add pre-dissolved tannin solution to the supernatant, while Stir while adding, so that the final concentration of tannin is 0.1%. After adding tannin, let it stand still, and after the formed tannin-enzyme complex is precipitated, carefully remove the supernatant by siphon method to obtain the lower layer compound The concentrated solution of the compound; the concentrated solution of the lower complex is separated and washed to prepare an enzyme paste, and the enzyme paste is placed in a low-temperature freezer below 12°C for 20 h...

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Abstract

The invention discloses a method for extracting and obtaining a bromelain from pineapple peel residues, which comprises the following steps of: cleaning pineapple peel residues, putting the pineapplepeel residues into a crushing and filtering device for multi-stage crushing, adding 0.05-0.1 % of ascorbic acid and 0.5-1 Kg of distilled water, and finally obtaining filtrate through extrusion and filtration; adding 0.03-0.05 % of calcium propionate into the filtrate for corrosion prevention, stirring and centrifuging the filtrate at a high speed for 10-15 minutes at a stirring speed of 4000-5000r / min, taking supernatant, adding pre-dissolved tannin solution into the supernatant, stirring while leading the final concentration of the tannin to be 0.1-0.2 %, and allowing the solution to standafter adding the tannin. The enzyme activity of the bromelain prepared from the pineapple peel residues is good, the purity is high, and the utilization rate of pineapple peel residues is improved.

Description

technical field [0001] The invention relates to the technical field of extracting bromelain, in particular to a method for extracting and obtaining bromelain from pineapple pomace. Background technique [0002] Bromelain (bromelain, EC3.4.22.33) is a proteolytic enzyme present in pineapple plants (including fruits, stems, handles and leaves). It has multiple isoenzymes, and its active center contains sulfhydryl (-SH), so A sulfhydryl protease. Bromelain is widely used in medicine, food, cosmetics, textile, feed, leather and other industries. It can decompose fibrin, hemoglobin and other proteins, and can dissolve fibrin and blood clots with less side effects, thereby improving body fluid circulation, increasing tissue permeability, and causing inflammation and edema to subside; combined with antibiotics and chemotherapy drugs It can promote the penetration and diffusion of drugs to the lesion, thereby enhancing the curative effect. [0003] However, in the existing bromel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12M1/33C12M1/12C12M1/00
Inventor 周伟李积华曹玉坡刘义军黄晓兵廖良坤彭芍丹张利
Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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