Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing small-molecular weight collagen active peptide through enzymolysis of yak bone and skin

A small molecular weight, collagen technology, applied in the field of bioengineering, to achieve high yield, small molecular weight, and easy absorption and utilization

Inactive Publication Date: 2016-10-12
肖建喜 +1
View PDF5 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because of the toughness and hardness of yak bones, the existing technology to extract collagen encounters certain challenges.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing small-molecular weight collagen active peptide through enzymolysis of yak bone and skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preferred embodiment 1 of the present invention provides a method for enzymatically hydrolyzing yak bone skin to prepare small molecular weight collagen active peptides. The steps are as follows:

[0025] (1) Raw material handling

[0026] The raw materials are cleaned, foreign matter is removed, dried at room temperature, and pulverized by a pulverizer to obtain a powder with a particle size of 50 mesh;

[0027] (2) The first enzyme-directed enzymatic hydrolysis

[0028] Get 1g of powder of yak bone, disperse in the solution of papain with the ratio of material to liquid weight ratio 1:3, the dosage of papain is 4000U / g, add citric acid-disodium hydrogen phosphate buffer solution to adjust pH to 5, control The pressure of the ultra-high pressure reactor is 300Mpa, and the enzymatic hydrolysis is performed for 2hrs at a temperature of 45°C;

[0029] (3) The second enzyme directional enzymatic hydrolysis

[0030] Adjust the pH of the solution after papain enzymolysis ...

Embodiment 2

[0034] The preferred embodiment 2 of the present invention provides a method for enzymatically hydrolyzing yak bone skin to prepare small molecular weight collagen active peptides. The steps are as follows:

[0035] (1) Raw material handling

[0036] The raw materials are cleaned, foreign matter is removed, dried at room temperature, and pulverized by a pulverizer to obtain a powder with a particle size of 100 mesh;

[0037] (2) The first enzyme-directed enzymatic hydrolysis

[0038] Take 10g of yak bone powder, and disperse it in the trypsin solution at a ratio of 1:5 by weight of material to liquid. The pressure of the reactor is 200Mpa, and the enzymatic hydrolysis is performed for 1.5hrs at a temperature of 45°C;

[0039] (3) The second enzyme directional enzymatic hydrolysis

[0040] Adjust the pH of the solution after trypsin enzymolysis to the optimum pH of pepsin 2.4, add pepsin, wherein the amount of pepsin is 1000U / g, control the pressure of the ultra-high pressur...

Embodiment 3

[0044] Preferred embodiment 3 of the present invention provides a method for enzymatically hydrolyzing yak bone skin to prepare small molecular weight collagen active peptides. The steps are as follows:

[0045] (1) Raw material handling

[0046] The raw materials are cleaned, foreign matter is removed, dried at room temperature, and pulverized by a pulverizer to obtain a powder with a particle size of 50 mesh;

[0047] (2) The first enzyme-directed enzymatic hydrolysis

[0048] Get the powder 10g of yak hide, with the ratio of solid-liquid weight ratio 1:3, be dispersed in the solution of papain, the consumption of papain is 4000U / g, add citric acid-disodium hydrogen phosphate buffer solution to adjust pH to 5.0, control The pressure of the ultra-high pressure reactor is 250Mpa, and the enzymatic hydrolysis is performed for 1hrs at a temperature of 45°C;

[0049] (3) The second enzyme directional enzymatic hydrolysis

[0050] Adjust the pH of the solution after papain enzy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing small-molecular weight collagen active peptide through enzymolysis of yak bone and skin. The method comprises the steps of raw material treatment, selective enzymatic hydrolysis of a first enzyme, selective enzymatic hydrolysis of a second enzyme and purifying treatment, wherein the first enzyme is one or more of papain, pepsase, alkaline protease, neutral protease, bromelain and trypsin; and the second enzyme is one or more of papain, pepsase, bromelain and trypsin. In the invention, the collagen peptide is directly extracted and prepared by use of an ultrahigh-pressure compound enzyme; the method has the advantages of simple process, short time, low energy consumption, low cost and greenness and environmental friendliness; and the prepared collagen peptide is invariable and has high yield, small molecular weight and high activity and is easily absorbed and utilized by a human body.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing small-molecular-weight collagen active peptides by stepwise and directed enzymatic hydrolysis of yak bone skin with compound enzymes. Background technique [0002] Collagen is a biological polymer that plays an important role in binding tissues in animal cells. As one of the most critical raw materials in the biotechnology industry, collagen is in huge demand, and its applications include biomedicine, cosmetics, health products, food and research purposes. Collagen can be divided into fish scale collagen, skin collagen and bone collagen according to the production raw materials; according to the molecular weight of the product, it can be divided into large molecular weight collagen and small molecular weight collagen peptides, among which small molecular weight collagen peptides are easier It is absorbed and utilized by the human body, so it has hig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC12P21/06C07K14/78
Inventor 肖建喜杨德庆李婷婷李红霞
Owner 肖建喜
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com