The invention discloses a preparation method and a product of low-pro-inflammatory reaction
donkey-hide
gelatin peptide. The preparation method comprises the following steps: (1) crushing
donkey-hide
gelatin blocks; (2) co-dissolving the crushed
colla corii asini obtained in the step (1) in non-
specific enzyme chain
enzyme protease E, and carrying out enzymolysis for 3-5 hours under an enzymolysis condition, so as to obtain an enzymolysis
colla corii asini solution; and (3) adding
protease into the enzymatic
hydrolysate obtained in the step (2) for enzymolysis, wherein the molecular weight of an enzymolysis product is made to range from 1000 Da to 3000 Da, high-temperature
enzyme deactivation is carried out, the
donkey-hide
gelatin enzymatic
hydrolysate is obtained, and (4) the donkey-hide gelatin enzymatic
hydrolysate in the step (3) is freeze-dried, and the donkey-hide gelatin
peptide is obtained. On one hand, the anti-
inflammatory response capability of the donkey-hide gelatin is reduced, on the other hand, the anti-oxidation capability of the product is provided, and meanwhile, the relatively strong positive immunoregulation capability is still maintained, so that the side effects of the donkey-hide gelatin and the donkey-hide
gelatin hydrolysate, which cause
inflammation, are reduced, the phenomenon of getting inflamed is reduced, the use limitation of the donkey-hide gelatin is reduced, and the donkey-hide gelatin is
safer to use.