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Preparation method and product of low-pro-inflammatory reaction donkey-hide gelatin peptide

A technology of inflammatory response and gelatin peptide, applied in the field of biochemistry, can solve the problems of aggravating inflammatory response, affecting the immune system, prolonging time, etc., to achieve the effect of reducing the side effects of inflammation, reducing restrictions, and using safety.

Pending Publication Date: 2022-01-14
武汉珈诗丽科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, donkey-hide gelatin is used to treat many local inflammations, such as urinary tract infection, gynecological inflammation, etc. At the same time, donkey-hide gelatin may further affect the immune system when the systemic immune system is stimulated, thereby aggravating systemic inflammation, manifested as Getting angry or inflammatory reaction aggravated and prolonged

Method used

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  • Preparation method and product of low-pro-inflammatory reaction donkey-hide gelatin peptide
  • Preparation method and product of low-pro-inflammatory reaction donkey-hide gelatin peptide
  • Preparation method and product of low-pro-inflammatory reaction donkey-hide gelatin peptide

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preparation example Construction

[0022] The invention provides a preparation method of donkey-hide gelatin peptide with low pro-inflammatory response, comprising the following steps:

[0023] (1) Superfinely pulverizing donkey-hide gelatin blocks through a 500-mesh sieve to obtain superfine donkey-hide gelatin powder;

[0024] (2) Mix the donkey-hide gelatin superfine powder obtained in step (1) with the non-specific enzyme chain enzyme protease E according to the mass ratio of 2000-2500:1, add 1.5 to 2 times the mass of donkey-hide gelatin superfine powder and dissolve it in warm water at 38°C to 42°C, Enzymatic hydrolysis for 3 to 5 hours to obtain sugar enzymatic hydrolysis glue;

[0025] The non-specific enzyme protease E (Pronase E) releases N-sugar chains and obtains sugar chains at the reducing end while hydrolyzing peptide bonds, improving the antioxidant properties of the final product and reducing inflammatory reactions.

[0026] (3) Adding protease to the enzymatic hydrolyzed gelatin solution obta...

Embodiment 1

[0032] A preparation method of donkey-hide gelatin peptide with low pro-inflammatory response, comprising the following steps:

[0033] (1) Superfinely pulverizing donkey-hide gelatin blocks through a 500-mesh sieve to obtain superfine donkey-hide gelatin powder;

[0034] (2) Mix the donkey-hide gelatin superfine powder obtained in step (1) with the non-specific enzyme chain enzyme protease E according to the mass ratio of 2000:1, add 1.5 times the mass of donkey-hide gelatin superfine powder in warm water at 40°C to dissolve, and enzymatically hydrolyze for 4 hours. Stir to maintain the temperature, and monitor the pH value so that the pH value is maintained between 7 and 8, and the sugar enzymatic hydrolysis glue is obtained;

[0035] (3) Adding protease to the enzymatic hydrolyzed gelatin solution obtained in step (2) for enzymatic hydrolysis, so that the molecular weight of the hydrolyzed product is between 1000Da and 3000Da, and inactivating the enzyme at high temperature...

Embodiment 2

[0039] A preparation method of donkey-hide gelatin peptide with low pro-inflammatory response, comprising the following steps:

[0040] (1) Superfinely pulverizing donkey-hide gelatin blocks through a 500-mesh sieve to obtain superfine donkey-hide gelatin powder;

[0041] (2) Mix the donkey-hide gelatin superfine powder obtained in step (1) with the non-specific enzyme chain enzyme protease E according to the mass ratio of 2000:1, add the donkey-hide gelatin superfine powder 2 times the mass of 38 ℃ warm water to dissolve, and enzymatically hydrolyze for 5 hours. Stir to maintain the temperature, and monitor the pH value so that the pH value is maintained between 7 and 8, and the sugar enzymatic hydrolysis glue is obtained;

[0042] (3) Adding protease to the enzymatic hydrolyzed gelatin solution obtained in step (2) for enzymatic hydrolysis, so that the molecular weight of the hydrolyzed product is between 1000Da and 3000Da, and inactivating the enzyme at high temperature to ...

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Abstract

The invention discloses a preparation method and a product of low-pro-inflammatory reaction donkey-hide gelatin peptide. The preparation method comprises the following steps: (1) crushing donkey-hide gelatin blocks; (2) co-dissolving the crushed colla corii asini obtained in the step (1) in non-specific enzyme chain enzyme protease E, and carrying out enzymolysis for 3-5 hours under an enzymolysis condition, so as to obtain an enzymolysis colla corii asini solution; and (3) adding protease into the enzymatic hydrolysate obtained in the step (2) for enzymolysis, wherein the molecular weight of an enzymolysis product is made to range from 1000 Da to 3000 Da, high-temperature enzyme deactivation is carried out, the donkey-hide gelatin enzymatic hydrolysate is obtained, and (4) the donkey-hide gelatin enzymatic hydrolysate in the step (3) is freeze-dried, and the donkey-hide gelatin peptide is obtained. On one hand, the anti-inflammatory response capability of the donkey-hide gelatin is reduced, on the other hand, the anti-oxidation capability of the product is provided, and meanwhile, the relatively strong positive immunoregulation capability is still maintained, so that the side effects of the donkey-hide gelatin and the donkey-hide gelatin hydrolysate, which cause inflammation, are reduced, the phenomenon of getting inflamed is reduced, the use limitation of the donkey-hide gelatin is reduced, and the donkey-hide gelatin is safer to use.

Description

technical field [0001] The invention belongs to the field of biochemistry, and more specifically relates to a preparation method and product of donkey-hide gelatin peptide with low pro-inflammatory response. Background technique [0002] Donkey-hide gelatin is a gelatin block made from donkey skin of equine animals. It is sweet in taste and flat in nature. It enters the lungs, liver and kidney meridian. material. Modern pharmacological studies have confirmed that donkey-hide gelatin has a wide range of clinical medical and health functions such as blood tonifying and hemostasis, anti-aging, anti-shock, anti-tumor, enhancing immunity, promoting calcium absorption and storage, and more and more studies on its active ingredients and pharmacological effects common concern in modern medicine. [0003] Modern scientific research has confirmed that donkey-hide gelatin and its hydrolyzed product, donkey-hide gelatin peptide, have a positive immune regulation effect. On the one ha...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P37/04
CPCC12P21/06A61K38/012A61P37/04Y02A50/30
Inventor 樊锋凯颜婷婷樊广浩
Owner 武汉珈诗丽科技有限公司
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