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Fish gelatin hydrolysate with oral hypoglycemic activity and application thereof

A technology for hydrolyzed products and fish gelatin, which is applied in the directions of medical preparations containing active ingredients, applications, hydrolyzed protein components, etc., can solve the problems of large environmental pollution, complicated operation, long time consumption, etc. The method is simple and the effect of reducing the production cost

Pending Publication Date: 2021-10-22
镇江市天益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Many reports at home and abroad use a strong acid-base method to treat fish skin, using acid or alkali to extract collagen under certain external conditions. Danger

Method used

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  • Fish gelatin hydrolysate with oral hypoglycemic activity and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of fish gelatin hydrolyzate with oral hypoglycemic activity as described above, comprising the steps of:

[0022] 1) Use fish gelatin mixed with 0.01% (w / w) papain and neutral protease (1:1) at pH 5.5 and temperature environment of 55°C, while adding 0.1% (w / w) phein and α - naphthalene flavonoids (1:1), enzymatic hydrolysis, reacted for 0.5 hours to form fish gelatin product A;

[0023] 2) Mix fish gelatin product A with 0.02% (w / w) alkaline protease and pepsin (1:1) at pH 6.5 and temperature environment of 60°C, while adding 0.25% (w / w) zealin And baicalin flavone I (1:1), continue enzymolysis, react 1.0 hours to form fish gelatin final product, make finished product;

Embodiment 2

[0025] A preparation method of fish gelatin hydrolyzate with oral hypoglycemic activity as described above, comprising the steps of:

[0026] 1) Use fish gelatin mixed with 0.02% (w / w) papain and neutral protease (2:1) at pH 6.0 and temperature environment of 60°C, while adding 0.2% (w / w) phin and α - naphthalene flavonoids (2:1), enzymatic hydrolysis, reacted for 1.0 hours to form fish gelatin product A;

[0027] 2) Mix fish gelatin product A with 0.01% (w / w) alkaline protease and pepsin (1:2) at pH 6.0 and a temperature environment of 65°C, while adding 0.2% (w / w) zealin And baicalin flavone I (2:1), continue enzymolysis, react 0.5 hour to form fish gelatin final product, make finished product;

Embodiment 3

[0029] C57 mice fed with high-fat and high-sugar diet were used to establish a hyperglycemia model. The mice were free to eat normal and normal feed as the control group. The mice were free to eat high-fat and high-sugar diet for 16 weeks as the model group. The mice were free to eat high-fat and high-sugar Feed for 16 weeks and freely drink fish gelatin hydrolyzate aqueous solution (5%, v / v) at the same time as the intervention group, and mice freely eat high-fat and high-sugar feed for 16 weeks and freely drink sildenatine (2.5%, v / v) for 16 weeks. positive drug group. After 16 weeks of eating, 24 hours after eating, blood was collected to detect fasting blood glucose level and fasting glycated hemoglobin level. Experimental results such as figure 1 As shown, it was found that fish gelatin hydrolyzate significantly reduced blood glucose levels in mice, and the hypoglycemic effect was equivalent to that of the positive drug sildenatine.

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Abstract

The invention discloses a fish gelatin hydrolysate with oral hypoglycemic activity and an application thereof, and the fish gelatin hydrolysate is mainly prepared by the following method: 1) mixing fish gelatin with papain and neutral protease, adding phellinus linteus ketone and alpha-naphthoflavone at the same time, and performing enzymolysis to form a fish gelatin product A; and 2) mixing the fish gelatin product A with alkaline protease and pepsase, adding herba lycopi flavone and scutellaria baicalensis flavone I at the same time, and continuing enzymolysis to form a fish gelatin final product. Compared with the prior art, the invention provides a novel fish gelatin hydrolysate which has a remarkable oral hypoglycemic effect and can be used for preparing health-care food. Besides, the preparation method is simple and easy to operate, the activity of hydrolase is remarkably promoted by utilizing flavone, the use amount of food-grade hydrolase is remarkably reduced, the hydrolysis reaction time is remarkably shortened, and the production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of fish gelatin hydrolyzate, and in particular relates to a fish gelatin hydrolyzate with oral hypoglycemic activity and application thereof. Background technique [0002] Hydrolyzed gelatin, also known as collagen polypeptide, is obtained by hydrolyzing collagen or gelatin to a certain extent. Compared with gelatin, hydrolyzed gelatin has the characteristics of strong water solubility and easy absorption by human body. In addition, hydrolyzed gelatin also has more physiological functions, such as anti-hypertension, prevention and treatment of osteoporosis, treatment of gastric ulcer, etc., and also has anti-aging, anti-oxidation and other functions. In recent years, the production of hydrolyzed gelatin has attracted more and more attention. The rapid development of the fish processing industry has produced a large number of by-products, which mainly include fish skin gelatin, accounting for about 10% of t...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23L33/18A61K38/01A61P3/10
CPCC12P21/06A23L33/18A61K38/014A61P3/10A23V2002/00A23V2200/328A23V2250/55
Inventor 陶旭魏雪倩
Owner 镇江市天益生物科技有限公司
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