Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing hydrolytic gelatin using combined enzyme

A technology of hydrolyzing gelatin and combining enzymes, applied in the preparation and fermentation of animal glue or gelatin, etc., can solve problems such as poor storage stability

Inactive Publication Date: 2004-07-14
北京华达杰瑞生物技术有限公司
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydrolyzed gelatin is a kind of polypeptide, which is easily decomposed by bacteria, etc., and has poor storage stability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 10 grams of gelatin and 90 mL of water to prepare an aqueous solution, adjust the pH of the system to 7 with citrate and phosphate buffer solution; heat up to 50°C to dissolve, then add 0.01 gram of trypsin and 0.1 gram of papain, and react for 1 hour. Heat at 90-100°C to kill enzymes, filter, concentrate, and dry to obtain gelatin degradation products. Determine the molecular weight of the products, and measure the stability and smell of the aqueous solution. The results are shown in Table 1.

Embodiment 2

[0019] Prepare an aqueous solution by adding 10 grams of gelatin and 90 mL of water, adjust the pH of the system to 7 with citrate and phosphate buffer solution; raise the temperature to 45°C to dissolve, then add 0.05 g of trypsin, react for 2 hours, and heat at 90-100°C Kill the enzyme, filter, cool down to 65°C, add 0.1 g of papain, react for 1 hour, heat at 90-100°C to kill the enzyme, filter, concentrate, and dry to obtain the product, measure the molecular weight of the product, and measure the stability and smell of the aqueous solution. taste, and the results are shown in Table 1.

Embodiment 3

[0021] Prepare an aqueous solution by adding 10 grams of gelatin and 90 mL of water, adjust the pH value of the system to 8 with citrate and phosphate buffer solution; heat up to 45°C to dissolve, then add 0.05 grams of trypsin and 0.2 grams of AS1.398 neutral protease, React for 3 hours, heat at 90-100°C to kill enzymes, filter, concentrate, and dry to obtain the gelatin degradation product. Determine the molecular weight of the product, and measure the stability and smell of the aqueous solution. The results are shown in Table 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The preparation process of hydrolytic gelatin with combined enzyme includes compounding water solution with gelatin material, regulating pH value with citric acid and phosphate buffering system, heating to reaction temperature, throwing enzyme via stirring, heating to killing enzyme, filtering, concentrating and drying to obtain hydrolytic gelatin. The combined enzyme contains any two of neutral proteinase containing AS1.398, papain, trypase and pineapple proteinase; the reaction has temperature suitable for the enzyme components, system pH value of 5-8.5 and reaction period of 0.5-5 hr; and the single enzyme adding amount is 0.01-2 wt% of gelatin. The enzyme components are added after being mixed or successively to degrade gelatin, and the process can decrease molecular weight of hydrolytic gelatin, raise hydrolytic gelatin solution stability and eliminate bad smell.

Description

technical field [0001] The invention relates to a method for preparing hydrolyzed gelatin by degrading gelatin with combined protease. The invention can reduce the molecular weight of the hydrolyzed gelatin, improve the stability of the hydrolyzed gelatin solution, and eliminate the fishy smell. Background technique [0002] Because hydrolyzed gelatin has the same chemical composition as gelatin, it maintains the inherent nutritional function of gelatin, and because of its low molecular weight, it increases its solubility in water and is easier to be absorbed by the human body, thereby further exerting its nutritional function. Not only in the fields of food, medicine, and daily chemicals, but also in the fields of textiles, printing and dyeing, coatings, papermaking, and chemicals. There are three ways to prepare hydrolyzed gelatin by enzymatic degradation of gelatin, namely acid method, alkali method and enzymatic method. Among them, the acid method and alkali method are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09H3/00C12P21/00
Inventor 黄雅钦黄明智
Owner 北京华达杰瑞生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com