Method for preparing hydrolytic gelatin using combined enzyme

Abstract

The preparation process of hydrolytic gelatin with combined enzyme includes compounding water solution with gelatin material, regulating pH value with citric acid and phosphate buffering system, heating to reaction temperature, throwing enzyme via stirring, heating to killing enzyme, filtering, concentrating and drying to obtain hydrolytic gelatin. The combined enzyme contains any two of neutral proteinase containing AS1.398, papain, trypase and pineapple proteinase; the reaction has temperature suitable for the enzyme components, system pH value of 5-8.5 and reaction period of 0.5-5 hr; and the single enzyme adding amount is 0.01-2 wt% of gelatin. The enzyme components are added after being mixed or successively to degrade gelatin, and the process can decrease molecular weight of hydrolytic gelatin, raise hydrolytic gelatin solution stability and eliminate bad smell.

Description

technical field

[0001] The invention relates to a method for preparing hydrolyzed gelatin by degrading gelatin with combined protease. The invention can reduce the molecular weight of the hydrolyzed gelatin, improve the stability of the hydrolyzed gelatin solution, and eliminate the fishy smell. Background technique

[0002] Because hydrolyzed gelatin has the same chemical composition as gelatin, it maintains the inherent nutritional function of gelatin, and because of its low molecular weight, it increases its solubility in water and is easier to be absorbed by the human body, thereby further exerting its nutritional function. Not only in the fields of food, medicine, and daily chemicals, but also in the fields of textiles, printing and dyeing, coatings, papermaking, and chemicals. There are three ways to prepare hydrolyzed gelatin by enzymatic degradation of gelatin, namely acid method, alkali method and enzymatic method. Among them, the acid method and alkali method are ...