Facultative anaerobic benzene compounds-degrading bacterium

A benzene-based compound and facultative anaerobic technology, applied in the direction of bacteria, microorganisms, water pollutants, etc., can solve the problems of low degradation reaction capacity, incomplete understanding of anaerobic degradation mechanism, and difficult to complete degradation, so as to achieve environmental adaptation strong effect

Inactive Publication Date: 2010-11-24
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology

However, the rapid consumption of dissolved oxygen in groundwater creates a large number of anaerobic zones for microorganisms. Therefore, many studies have focused on the anaerobic biodegradation of benzene series compounds in the past two decad

Method used

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  • Facultative anaerobic benzene compounds-degrading bacterium
  • Facultative anaerobic benzene compounds-degrading bacterium
  • Facultative anaerobic benzene compounds-degrading bacterium

Examples

Experimental program
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Embodiment Construction

[0015] 1 Screening method for facultative anaerobic degrading bacteria of benzene series compounds

[0016] 1.1 Material preparation

[0017] 1.1.1 Bacteria source

[0018] Collect soil samples 5-10cm below the surface in the oil-contaminated area of ​​Daqing Oilfield.

[0019] 1.1.2 Medium

[0020] Inorganic salt separation medium: 0.42g L -1 NaNO 3 , 0.27g L -1 NH 4 Cl, 7.9g L -1 Na 2 HPO 4 12H 2 O, 1.5g·L -1 K H 2 PO 4 , 1.0g·L -1 MgSO 4 ·7H 2 O, 1.0mL·L -1 Trace element solution (the ratio of trace element solution is: 2.14g L -1 ZnCl 2 , 2.5g·L -1 MnCl 2 4H 2 O, 0.3g·L -1 CoCl 2 ·6H 2 O, 0.2g·L -1 CuCl 2 2H 2 O, 0.4g L -1 NaMoO 4 2H 2 O, 4.5g·L -1 CaCl 2 2H 2 O, 2.9g L -1 FeCl 3 ·6H 2 O, 1.0g·L -1 h 3 BO 3 , 0.1g L -1 KI, 1L deionized water), 0.472g L -1 Sodium succinate, 1L distilled water, pH=7.0.

[0021] Agar medium: 1L inorganic salt separation medium + 20g L -1 agar.

[0022] LB medium: 1.0g·L -1 Yeast powder (YEAST EXTRA...

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Abstract

The invention belongs to the technical field of microbe degradation, and discloses a facultative anaerobic benzene compounds-degrading bacterium, which is characterized in that the bacterium is Staphylococcus sciuri HBSD-A CCTCC No.M209283 and has been preserved in China Center for Type Culture Collection with the collection number of CCTCC No.M209283 on November 27, 2009. The bacterium has the milk white, round, opaque, sawteeth-edge, slightly convex and smooth-surface colonial morphology; the cells are spherical, Gram-positive and immobile; and the bacterium has the main biochemical characteristics of oxidase-negative and catalase-positive bacterium, utilization of glucose, fructose and sucrose instead of maltose, denitrification-positive and gelatin hydrolysate-positive bacterium, facultative anaerobic bacterium and growth temperature between 4 and 41 DEG C. The bacterium can efficiently degrade methylbenzene and dimethylbenzene under the anaerobic condition, can degrade benzene or mesitylene, and has the maximum 3d degradation rate of 89.0 percent for benzene compounds at the concentration about 175.8mg/L.

Description

technical field [0001] The invention belongs to the technical field of degrading microorganisms, and also belongs to the technical field of biological treatment of environmental pollutants, and specifically relates to a facultative anaerobic degrading bacterium for benzene series compounds. Background technique [0002] Benzene compounds are a class of volatile monocyclic aromatic hydrocarbon compounds, including benzene, toluene, ethylbenzene, o-, m-, and p-xylene, etc. In addition to existing in gasoline fuel, benzene compounds are also widely used as raw materials Manufacturing of rubber, lubricants, dyes, detergents, pharmaceuticals and pesticides (Romy Chakraborty, JohnD.Coates.2005.Hydroxylation and Carboxylation-Two Crucial Steps of Anaerobic Benzene Degradation by DechloromonasStrain RCB. Applied and environmental microbiology, Sept. p.5427-5432). The content of benzene series compounds in petroleum hydrocarbons is not high but it is paid much attention to. It is th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/44C02F101/36A62D101/20
Inventor 吕敦玉周建伟余楚周爱国蔡鹤生李萍童蕾
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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