Facultative anaerobic benzene compounds-degrading bacterium
A benzene-based compound and facultative anaerobic technology, applied in the direction of bacteria, microorganisms, water pollutants, etc., can solve the problems of low degradation reaction capacity, incomplete understanding of anaerobic degradation mechanism, and difficult to complete degradation, so as to achieve environmental adaptation strong effect
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[0015] 1 Screening method for facultative anaerobic degrading bacteria of benzene series compounds
[0016] 1.1 Material preparation
[0017] 1.1.1 Bacteria source
[0018] Collect soil samples 5-10cm below the surface in the oil-contaminated area of Daqing Oilfield.
[0019] 1.1.2 Medium
[0020] Inorganic salt separation medium: 0.42g L -1 NaNO 3 , 0.27g L -1 NH 4 Cl, 7.9g L -1 Na 2 HPO 4 12H 2 O, 1.5g·L -1 K H 2 PO 4 , 1.0g·L -1 MgSO 4 ·7H 2 O, 1.0mL·L -1 Trace element solution (the ratio of trace element solution is: 2.14g L -1 ZnCl 2 , 2.5g·L -1 MnCl 2 4H 2 O, 0.3g·L -1 CoCl 2 ·6H 2 O, 0.2g·L -1 CuCl 2 2H 2 O, 0.4g L -1 NaMoO 4 2H 2 O, 4.5g·L -1 CaCl 2 2H 2 O, 2.9g L -1 FeCl 3 ·6H 2 O, 1.0g·L -1 h 3 BO 3 , 0.1g L -1 KI, 1L deionized water), 0.472g L -1 Sodium succinate, 1L distilled water, pH=7.0.
[0021] Agar medium: 1L inorganic salt separation medium + 20g L -1 agar.
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