Method for checking purity of hybridized pepper seeds

Abstract

The invention discloses a method for checking the purity of hybridized pepper seeds. The method is technically characterized by comprising the following steps of: soaking seeds into an acetone solution for 5-6 hours so that the seeds are degreased; taking out the seeds and airing; cutting one seed into three blocks and placing the seeds into a centrifugal tube; adding 90 microliters of extraction liquid; placing at 5 DEG C for 12 hours; extracting liquid supernatant to obtain seed storage protein extraction liquid; placing the liquid on a plate, filling glue, carrying out sample application and electrophoresis, taking the glue, dyeing and manufacturing a wafer to obtain a sample electrophoresis spectrum band; carrying out the method to respectively obtain a standard electrophoresis spectrum band and a detected sample electrophoresis spectrum band; and comparing the detected sample electrophoresis spectrum band with the standard electrophoresis spectrum band to obtain the purity of seed samples to be detected. The method disclosed by the invention is simple and convenient, rapid and accurate; and the process is easy to master and control. The method is particularly suitable for checking the purity of a hot pepper*sweet pepper type hot pepper breed combination.

Description

technical field [0001] The invention relates to a method for inspecting the purity of hybrid pepper seeds, in particular to a method for inspecting the purity by using stored protein contained in pepper seeds, and belongs to the field of vegetable seed quality inspection. Background technique [0002] Capsicum is an important vegetable cultivar. my country is not only one of the origin centers of pepper, but also a big country of pepper cultivation and consumption. Pepper seed production mainly adopts artificial detasselling and direct pollination of male sterile lines. CMS seed production can only be used in sharp pepper × sharp pepper; sharp pepper × sweet pepper and sweet pepper × sweet pepper are artificially detasselled Seed production methods for pollination. Due to the influence of climate, seed producers' management level and human factors, the seed production method of artificial detasseling and pollination causes the purity of a large part of pepper seeds to be l...

AI Technical Summary

Problems solved by technology

Due to the long period of field planting identification, high cost, and great influence of natural climate conditions, it cannot meet the needs of seed sales in the current year, resulting in the method of selling seeds in stock every other year.

The indoor molecular-specific labeling method has higher technical requirements for mechanical equipment and operators, especially for a product that needs to screen a specific primer, which is difficult for medium-sized and above-scale vegetable companies

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