Side sequence of exogenous insert of transgene paddy strain Kemingdao 1

A technology of transgenic rice and flanking sequence, which is applied in the direction of genetic engineering, plant genetic improvement, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2008-08-13
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the analysis of existing patents and documents, it is found that there are no articles or pat

Method used

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  • Side sequence of exogenous insert of transgene paddy strain Kemingdao 1
  • Side sequence of exogenous insert of transgene paddy strain Kemingdao 1
  • Side sequence of exogenous insert of transgene paddy strain Kemingdao 1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Cloning of the flanking sequence of the foreign insert of the transgenic rice line KMD1 (KMD1)

[0031] 1. Experimental materials

[0032] 1. Plant materials

[0033] Transgenic rice: KMD1, a genetically modified rice line.

[0034] Conventional rice: Xiushui 11.

[0035] 2. Enzymes and reagents

[0036] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000Marker were purchased from Dalian Bao Biological Biological Engineering Co., Ltd.

[0037] Other biochemical reagents are imported aliquots or domestically produced analytical pure. The primers and degenerate primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0038] 3. Experimental equipment

[0039] PCR thermal cycler: Eppendorf Mastercycle gradient (Eppendorf co.)

[0040] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0041] DNA sequencer (Applied Biosystem 377 automatic fluorescence sequence analyzer)

[0...

Embodiment 2

[0082] Qualitative PCR detection method based on the flanking sequence of the foreign insert of the transgenic rice line KMD1 (KMD1)

[0083] 1. Experimental materials

[0084] 1. Plant materials

[0085] Transgenic rice: KMD1, a genetically modified rice line.

[0086] Conventional rice: Xiushui 11.

[0087] 2. Enzymes and reagents

[0088] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000Marker were purchased from Dalian Bao Biological Biological Engineering Co., Ltd. Other biochemical reagents are imported aliquots or domestically produced analytical grades. The primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0089] 3. Experimental equipment

[0090] PCR thermal cycler: Eppendorf Mastercycle gradient (Eppendorf co.)

[0091] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0092] DNA electrophoresis analysis system: Kodak EDAS 290 (Kodak co.)

[0093] Other in...

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Abstract

The invention relates to a side sequence of exogenous interpose fragments of transgene paddy rice line snout moth's larva-subduing rice. its 5' side sequence is shown as SEQ ID NO1 and its 3' side sequence is shown as SEQ ID NO2. The line specificity determine the nature and definite quantity PCR detection of sensitive idiosyncratic transgene paddy rice line snout moth's larva-subduing rice is constructed by using side sequence as aim DNA amplification fragments.

Description

Technical field [0001] The invention relates to a gene sequence in the field of biotechnology. Specifically, it relates to the flanking sequence of an exogenous insert fragment of a transgenic rice line KMD1 (KMD1). Background technique [0002] Rice is one of the most important food crops in my country. With the extensive research and application of transgenic technology in rice, many transgenic rice lines have been released for environmental release and applied for commercial planting. Strain-specific detection of genetically modified plants is an important technical basis for the supervision and management of genetically modified plants to ensure their healthy development. The flanking sequence of exogenous inserts of transgenic plants is one of the most important molecular characteristics of transgenic plant lines. Therefore, the flanking sequences of exogenous inserts are important technical data for establishing specific detection methods for transgenic plant lines. [0003...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/68
Inventor 谢家建王锡锋彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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