Domestication method of nostoc and application thereof
A technology of Nostoc and its cultivation method, which is applied in the domestication of Nostoc and its application fields, can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, etc., and achieve the solution of soil salinization, short growth cycle, The effect of increasing nitrogen and phosphorus content
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Embodiment 1
[0058] Treatment of mild and moderate saline-alkali areas around Yantai, Shandong
[0059] Step 1: Prepare the FN31 medium (basic medium) for cultivating Nostoc:
[0060] Potassium nitrate 0.2g / L Calcium chloride 0.027g / L
[0061] Dipotassium hydrogen phosphate 0.04g / L Sodium chloride 1.0g / L
[0062] Potassium dihydrogen phosphate 0.175g / L Ferric chloride 0.008g / L
[0063] Magnesium Sulfate 0.037g / L Naphthaleneacetic Acid 0.3mg / L
[0064] Trace elements 1.5ml / L
[0065] Wherein the trace element preparation method is:
[0066] Boric acid 286mg Copper sulfate 5mg
[0067] Manganese Chloride 115mg Ammonium Molybdate 3.7mg
[0068] Zinc sulfate 12mg Add distilled water to make up to 1L
[0069] The culture conditions are: temperature 24°C, light 8000 lux, pH: 8.0, growth cycle 8 days.
[0070] Step 2: Adaptation of Nostoc according to the use of the saline-alkali land:
[0071] (1). Salinity-tolerant acclimatization: For mild or moderate saline-alkali land, gradually inc...
Embodiment 2
[0094] Treatment of saline-alkali areas around Yantai, Shandong
[0095] Step 1: Prepare the medium (basic medium) for cultivating Nostoc:
[0096] Potassium nitrate 0.3g / L Calcium chloride 0.03g / L
[0097] Dipotassium hydrogen phosphate 0.058g / L Sodium chloride 0.5g / L
[0098] Potassium dihydrogen phosphate 0.25g / L Ferric chloride 0.001g / L
[0099] Magnesium Sulfate 0.05g / L Naphthaleneacetic Acid 0.4mg / L
[0100] Trace elements 0.5ml / L
[0101] The culture conditions are: temperature 25°C, light 9000 lux, pH: 7.5, growth cycle 8 days.
[0102] Step 2: Adaptation of Nostoc according to the use of the saline-alkali land:
[0103] (1). Salinity tolerance acclimatization:
[0104] Potassium nitrate 0.3g / L Calcium chloride 0.03g / L
[0105] Dipotassium hydrogen phosphate 0.058g / L Ferric chloride 0.001g / L
[0106] Potassium dihydrogen phosphate 0.25g / L Naphthaleneacetic acid 0.4mg / L
[0107] Magnesium sulfate 0.05g / L Trace elements 0.5ml / L
[0108] Sodium chloride graduall...
Embodiment 3
[0125] Treatment of saline-alkali areas around Yantai, Shandong
[0126] Step 1: Prepare the medium (basic medium) for cultivating Nostoc:
[0127] Potassium nitrate 0.4g / L Calcium chloride 0.005g / L
[0128] Dipotassium hydrogen phosphate 0.07g / L Sodium chloride 1.5g / L
[0129] Potassium dihydrogen phosphate 0.1g / L Ferric chloride 0.003g / L
[0130] Magnesium Sulfate 0.03g / L Naphthaleneacetic Acid 0.4mg / L
[0131] Trace elements 1ml / L
[0132] The culture conditions are: temperature 26°C, light 8000 lux, pH: 8.5, growth cycle 8 days.
[0133] Step 2: Adaptation of Nostoc according to the use of the saline-alkali land:
[0134] (1). Salinity tolerance acclimatization:
[0135] Potassium nitrate 0.4g / L Calcium chloride 0.005g / L
[0136] Dipotassium hydrogen phosphate 0.07g / L Ferric chloride 0.003g / L
[0137] Potassium dihydrogen phosphate 0.1g / L Naphthaleneacetic acid 0.4mg / L
[0138] Magnesium Sulfate 0.03g / L Trace Elements 1ml / L
[0139] Sodium chloride gradually incr...
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