Domestication method of nostoc and application thereof

A technology of Nostoc and its cultivation method, which is applied in the domestication of Nostoc and its application fields, can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, etc., and achieve the solution of soil salinization, short growth cycle, The effect of increasing nitrogen and phosphorus content

Inactive Publication Date: 2011-04-13
北京芳能科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) Among the existing main methods for controlling saline-alkali land, the water washing and salt discharge adopted by the physical method are likely to cause the loss of soil nutrients, and require large material and financial resources, while the fertilization or acid-base balance adopted by the chemical method is likely to cause soil compaction and Excessive governance costs
Although physical and chemical methods can temporarily change the salinity of the soil, they cannot essentially change the soil fertility of the original saline-alkali land.

Method used

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  • Domestication method of nostoc and application thereof
  • Domestication method of nostoc and application thereof
  • Domestication method of nostoc and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Treatment of mild and moderate saline-alkali areas around Yantai, Shandong

[0059] Step 1: Prepare the FN31 medium (basic medium) for cultivating Nostoc:

[0060] Potassium nitrate 0.2g / L Calcium chloride 0.027g / L

[0061] Dipotassium hydrogen phosphate 0.04g / L Sodium chloride 1.0g / L

[0062] Potassium dihydrogen phosphate 0.175g / L Ferric chloride 0.008g / L

[0063] Magnesium Sulfate 0.037g / L Naphthaleneacetic Acid 0.3mg / L

[0064] Trace elements 1.5ml / L

[0065] Wherein the trace element preparation method is:

[0066] Boric acid 286mg Copper sulfate 5mg

[0067] Manganese Chloride 115mg Ammonium Molybdate 3.7mg

[0068] Zinc sulfate 12mg Add distilled water to make up to 1L

[0069] The culture conditions are: temperature 24°C, light 8000 lux, pH: 8.0, growth cycle 8 days.

[0070] Step 2: Adaptation of Nostoc according to the use of the saline-alkali land:

[0071] (1). Salinity-tolerant acclimatization: For mild or moderate saline-alkali land, gradually inc...

Embodiment 2

[0094] Treatment of saline-alkali areas around Yantai, Shandong

[0095] Step 1: Prepare the medium (basic medium) for cultivating Nostoc:

[0096] Potassium nitrate 0.3g / L Calcium chloride 0.03g / L

[0097] Dipotassium hydrogen phosphate 0.058g / L Sodium chloride 0.5g / L

[0098] Potassium dihydrogen phosphate 0.25g / L Ferric chloride 0.001g / L

[0099] Magnesium Sulfate 0.05g / L Naphthaleneacetic Acid 0.4mg / L

[0100] Trace elements 0.5ml / L

[0101] The culture conditions are: temperature 25°C, light 9000 lux, pH: 7.5, growth cycle 8 days.

[0102] Step 2: Adaptation of Nostoc according to the use of the saline-alkali land:

[0103] (1). Salinity tolerance acclimatization:

[0104] Potassium nitrate 0.3g / L Calcium chloride 0.03g / L

[0105] Dipotassium hydrogen phosphate 0.058g / L Ferric chloride 0.001g / L

[0106] Potassium dihydrogen phosphate 0.25g / L Naphthaleneacetic acid 0.4mg / L

[0107] Magnesium sulfate 0.05g / L Trace elements 0.5ml / L

[0108] Sodium chloride graduall...

Embodiment 3

[0125] Treatment of saline-alkali areas around Yantai, Shandong

[0126] Step 1: Prepare the medium (basic medium) for cultivating Nostoc:

[0127] Potassium nitrate 0.4g / L Calcium chloride 0.005g / L

[0128] Dipotassium hydrogen phosphate 0.07g / L Sodium chloride 1.5g / L

[0129] Potassium dihydrogen phosphate 0.1g / L Ferric chloride 0.003g / L

[0130] Magnesium Sulfate 0.03g / L Naphthaleneacetic Acid 0.4mg / L

[0131] Trace elements 1ml / L

[0132] The culture conditions are: temperature 26°C, light 8000 lux, pH: 8.5, growth cycle 8 days.

[0133] Step 2: Adaptation of Nostoc according to the use of the saline-alkali land:

[0134] (1). Salinity tolerance acclimatization:

[0135] Potassium nitrate 0.4g / L Calcium chloride 0.005g / L

[0136] Dipotassium hydrogen phosphate 0.07g / L Ferric chloride 0.003g / L

[0137] Potassium dihydrogen phosphate 0.1g / L Naphthaleneacetic acid 0.4mg / L

[0138] Magnesium Sulfate 0.03g / L Trace Elements 1ml / L

[0139] Sodium chloride gradually incr...

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Abstract

The invention discloses a domestication method of nostoc and application thereof. The domestication method comprises the following steps of: preparing a specific culture medium; carrying out salt tolerance domestication, nitrogen and phosphorus content reduction domestication, domestication of adaptability to water to be treated, and the like on screened nostoc species; carrying out step-by-step amplification culture; then leading the nostoc species into a saline and alkaline land, wherein the nostoc is propagated in large quantities by utilizing photosynthesis and absorbs and fixes nitrogen and phosphorus elements contained in atmosphere and water sources; and after the nostoc is propagated, draining culture water for the nostoc, wherein the nostoc is deposited in soil and contains a large quantity of elements, i.e. nitrogen, phosphorus, and the like. After primary treatment is carried out by using the domestication method, the nitrogen contained in the soil is increased by 18-21 percent, the phosphorus is increased by 12-14 percent, and full salt content is reduced by 15-18 percent, therefore not only is nutrient element loss caused by a water washing method prevented, but also the hardening of the soil and the secondary pollution to the water sources and environments, which are caused by chemical fertilizer application, are prevented; and in addition, soil fertility is gradually enhanced and soil restoration is accelerated through multi-time treatment, therefore the purpose of improving an ecological environment is finally achieved.

Description

technical field [0001] The invention discloses a method for improving saline-alkali land and increasing soil fertility by using nitrogen-fixing blue-green algae, in particular to a method for treating saline-alkali land by using Nostoc algae. Background technique [0002] Saline-alkali lands are widely distributed around the world, covering more than 30 countries on six continents. It is mainly distributed in India, Pakistan, Russia, South Africa and other countries, with a total area of ​​about 956 million hectares. Among them, India and Pakistan have 8.09 million hectares, and Russia has 7.5 million hectares. The area of ​​saline-alkali land in the western United States accounts for about half of the irrigated area, and the output of saline-alkali is reduced by more than 25% every year. Land salinization is an important part of land desertification, which seriously threatens the local agricultural and animal husbandry production, the prosperity and development of the nat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C09K17/00C12R1/89C09K109/00
Inventor 单东杰李涛
Owner 北京芳能科技有限公司
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