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Broad-spectrum virulent cyanophage Me-ZS1 and application thereof

A cyanophage, me-zs1 technology, applied in the direction of bacteriophage, virus/bacteriophage, microorganism-based methods, etc., can solve the problems of application limitation, narrow algicidal spectrum, difficult screening and separation of cyanophage, etc., and achieve easy expansion The effect that culture, culture conditions are simple

Inactive Publication Date: 2020-06-12
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The screening and isolation of cyanophages are very difficult, and the cyanophages reported so far have strong host specificity, narrow algicidal spectrum, and limited applications.

Method used

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  • Broad-spectrum virulent cyanophage Me-ZS1 and application thereof
  • Broad-spectrum virulent cyanophage Me-ZS1 and application thereof
  • Broad-spectrum virulent cyanophage Me-ZS1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of cyanophage Me-ZS1

[0027] The water samples were collected from the pond near Cao Guangbiao Science and Technology Building, Ningbo University, Ningbo City, Zhejiang Province (North latitude: 29.9108051933; East longitude: 121.6313112778); the target algae was Microcystis elabena FACHB-916, and the specific preparation method was as follows:

[0028] (1) Activation of target algal species

[0029] Take 50 μL of Microcystis elabena FACHB-916 (Microcystis elabena FACHB-916) algae liquid, spread it on the BG11 medium plate containing 1.5% (W / V) agar, and place it in a light incubator for 15 days; The setting parameters of the box are: the temperature is 25°C, the light intensity is 2000lx, and the light-dark cycle is 12h:12h; single algae are picked and dropped into 5mL fresh BG11 medium for cultivation, and the algae are manually shaken 3 times a day to make them fully utilize nutrients ; After the logarithmic growth phase, inoculate the algae liquid in t...

Embodiment 2

[0043] Morphological observation of cyanophage Me-ZS1

[0044] The cyanophage Me-ZS1 isolated and purified in Example 1 was dropped on a copper grid, negatively stained with 2% uranyl acetate solution (w / v), and observed under an electron microscope (Hitachi H-7650) Morphology of cyanophages.

[0045] The result is as figure 1 As shown, the cyanophage Me-ZS1 has an icosahedral head structure and a slender tail, the head diameter is about 60nm, and the tail length is 260nm;

[0046] The purified cyanophages were infected and mixed with exponentially growing Microcystis aeruginosa, and the algal plaque experiment was carried out, and transparent algal plaques with uniform size and shape could be obtained. There was no halo around the algal plaques, and the edges were clear and regular.

Embodiment 3

[0048] Host range of cyanophages

[0049] The cyanophage Me-ZS1 infection liquid was added to the cyanobacteria liquid of 15 strains (Table 1) at a ratio of 1:2 (V:V), and the artificial infection experiment was carried out. FACHB-905, FACHB-925, FACHB-469, FACHB-942 strains of Microcystisaeruginosa, FACHB-908, FACHB-1112, FACHB-929 strains of M. M.flos-aquae FACHB-1028, M.elabena FACHB-916, Microcystis sp.7806 FACHB-915 strain, and Nostoc sp. (Nostoc sp.) FACHB-596 strain and Anabaenaflosaguas (Anabaenaflosaguas) FACHB-245 strain both have cracking effect. figure 2 Some microscopic photographs of algae infected by cyanophage Me-ZS1 are listed.

[0050] Table 1 Results of host range experiments of cyanophage Me-ZS1

[0051]

[0052]

[0053] "+" means infection, "-" means no infection

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Abstract

The invention relates to broad-spectrum virulent cyanophage Me-ZS1 and application thereof, and relates to biological treatment of water blooms, wherein the Me-ZS1 is preserved in China General Microbiological Culture Collection Center, and the serial number of the Me-ZS1 is CGMCC No. 16812. According to the invention, Me-ZS1 is cyanophage obtained by separating the first strain by taking the toxin-producing microcystis elabena as a target, and has cracking effects on Microcystis aeruginosa FACHB-905, FACHB-925, FACHB-469 and FACHB-942 strains under Chroococcales, M .wesenbergii FACHB-908, FACHB-1112 and FACHB-929 strains, M .flos-aquae FACHB-1028 strain, M .elabena FACHB-916 strain, Microcystis sp .7806 FACHB-915 strain, Nostoc sp. FACHB-596 strain under Nostocales, and Anabaena flosaguasFACHB-245 strain.

Description

technical field [0001] The invention relates to a broad-spectrum potent cyanophage, in particular to a broad-spectrum potent cyanophage and application thereof. Background technique [0002] With the rapid development of the economy, water pollution and eutrophication are becoming more and more serious, and "water blooms" frequently break out, among which cyanobacteria are the main algae that cause water blooms. "Water blooms" can cause a decrease in dissolved oxygen in water bodies, deterioration of water quality, release of unpleasant odors, release of algae toxins, and cause a series of problems such as mass death of fish and other organisms. Cyanotoxins can not only cause a large number of deaths of aquatic animals and plants, but also can inhibit the activity of protein phosphatase 2A of eukaryotes to cause liver cancer and so on. "Water bloom" is one of the most serious environmental disasters in the world today, seriously endangering water ecosystems and threatening ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/02C02F3/34C12R1/92
CPCC12N7/00C02F3/34C12N2795/10321C12N2795/10351C02F2103/007
Inventor 李登峰孙智同林威严小军童贻刚秦伟南许丽华
Owner NINGBO UNIV
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