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Nostoc sp. capable of high-efficiency degradation of polychlorinated biphenyl and application thereof

A technology of polychlorinated biphenyls and Nostoc, which is applied in the application field of microbial degradation of polychlorinated biphenyls, and can solve the problems of high cost, secondary pollution price, and no literature reports on polychlorinated biphenyls.

Active Publication Date: 2014-06-04
浙江省环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above physical or chemical methods all have the problem of producing secondary pollution or being expensive
Although domestic and foreign scholars have conducted research on the degradation of PCBs by microorganisms, there is no literature report on the degradation of PCBs by algae species in my country.

Method used

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  • Nostoc sp. capable of high-efficiency degradation of polychlorinated biphenyl and application thereof
  • Nostoc sp. capable of high-efficiency degradation of polychlorinated biphenyl and application thereof
  • Nostoc sp. capable of high-efficiency degradation of polychlorinated biphenyl and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Separation and screening of algal species

[0022] Soil sample: collected from a PCB-contaminated rice field in Taizhou City, Zhejiang Province.

[0023] Enrichment and separation of algal species: screened with liquid medium containing 10ppmPCBs.

[0024] (2) Preparation of culture medium

[0025] Weigh 1.5g NaNO 3 , 0.075g MgSO 4 ·7H 2 O, 0.006g Citric acid, 0.001g Na-EDTA, 0.04g K 2 HPO 4 ·3H 2 O, 0.006g Ferric ammonia citrate, 0.02g Na 2 CO 3 Add 850mL deionized water to the beaker, stir to dissolve, adjust the pH to 7.0, and sterilize in a high-pressure steam sterilizer for 30 minutes.

[0026] (3) Molecular identification of algal species

[0027] After 16SrDNA sequence analysis, the algal species was identified as: Nostoc PD-2 (Nostoc algae).

[0028] The 16SrDNA sequence of Nostoc algae was sequenced, and the sequence shown as SEQ ID NO.1 was obtained, and the 16SrDNA BLAST of this algae species was compared and analyzed on Genebank (results are s...

Embodiment 2

[0031] Example 2: Experimental results of PD-2 algae biodegrading 2mg / L PCB28, 29, 30, and 31 for one week

[0032] Nostoc sp.PD-2 (CCTCC No: M2013397) algae were placed in conical flasks at 25°C under 2000lux light and cultured with BG11 inorganic salt medium until logarithmic growth phase, and another clean conical flask was taken for each bottle Add 20mL of the aforementioned algae solution, add PCB28 working solution dissolved in methanol to each Erlenmeyer flask with an initial concentration of 100mg / L, add 400μL, so that the concentration of PCB28 in the system is 2mg / L. After culturing for 7 days, samples were taken, and the content of chloride ions was measured by mercury thiocyanate ferric photometry. The method of degrading PCB29, 30, and 31 for one week was the same as above. After 7 days, the masses of chloride ions removed by PCB28, 29, 30, and 31 were 10.7 μg, 8.25 μg, 6.49 μg, and 9.30 μg, respectively. Degradation results seefigure 1 .

[0033] The compositio...

Embodiment 3

[0038] Example 3: Experimental results of PD-2 algal species biodegrading PCB28, 29, 30, and 31 for one week when different concentrations of glucose were added to the system

[0039] Nostoc sp.PD-2 algae were placed in Erlenmeyer flasks at 25°C under 2000lux light conditions and cultured with BG11 inorganic salt medium until the logarithmic growth phase, and another clean Erlenmeyer flask was added to each bottle with 20mL of the above-mentioned algae liquid. Add the prepared glucose solution into two Erlenmeyer flasks, so that the concentrations of glucose in the system are 5mg / L and 50mg / L respectively, and add the working solution to make the concentrations of PCB28, 29, 30, and 31 in the system respectively 2mg / L . Cultured at 25°C and 2000 lux light for 7 days, samples were taken, and the ferric photometric method was adopted with mercury thiocyanate, and the culture medium without grape samples was set as the blank control group. When no glucose was added, the masses o...

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Abstract

The invention provides Nostoc sp. PD-2 capable of high-efficiency degradation of polychlorinated biphenyl and application of the same in microbial degradation of polychlorinated biphenyl. The Nostoc sp. PD-2 is preserved in China Center for Type Culture Collection, Wuhan University, Wuhan, China, 430072, on Sep. 8th, 2013, and has an accession number of CCTCC No. M2013397. The Nostoc sp. PD-2 provided by the invention can effectively degrade PCB28, 29, 30 and 31; and after exposure for one week, degradation efficiency of the Nostoc sp. PD-2 on PCB28 exceeds 60%. Thus, the Nostoc sp. PD-2 is widely applicable to degradation of polychlorinated biphenyl in an environment and has important application prospects.

Description

(1) Technical field [0001] The invention relates to Nostoc sp. PD-2 capable of efficiently degrading polychlorinated biphenyls and its application in microbial degradation of polychlorinated biphenyls. (2) Background technology [0002] Polychlorinated biphenyls (PCBs) are a group of chlorinated aromatic hydrocarbons with 209 homologues formed by chemically catalyzing the substitution of 1 to 10 chlorine atoms for hydrogen on the biphenyl molecule at different positions. Due to the rapid development of human industry, PCB has become a worldwide pollution. For example, motor factories that use PCB as insulating oil, chemical factories that use PCB as heat carrier and lubricating oil in large quantities, paper mills, especially recycled paper mills. The corrosion-resistant coating of ships contains PCB, which is also a considerable source of pollution when dissolved by seawater. PCBs from these pollution sources enter the water system in the form of waste oil, slurry, paint ...

Claims

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Application Information

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IPC IPC(8): C12N1/12A62D3/02C12R1/01A62D101/22
Inventor 张杭君蒋晓军王佳鲁莉萍肖文丰
Owner 浙江省环境科技有限公司
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