Nostoc sphaeroidskutz colonization induction method

Abstract

The invention relates to a nostoc sphaeroids kutz algae colonization induction method. The nostoc sphaeroids kutz algae colonization induction method comprises the following steps: a nostoc sphaeroidskutz group is induced into a grey green group with an algae colonization release signal; a mild tearing method is utilized, so that the group completely loses osmotic protection, and under the assistance of ventilation and multiple induction of violent condition changes before and after ventilation, algae filaments are broken and differentiated into nostoc sphaeroides colonization sections. The method has the beneficial effects that under carbon source deficiency under absolute vacuum and under the synergistic effect of temperature and illumination conditions for enhancing photosynthesis of the nostoc sphaeroides are supplemented, nostoc sphaeroides group pigments are induced to be degraded into nostocsphaeroides groups with algal colonization segment release signals, ventilation induction after tearing is adopted, the algae filaments can smoothly break colloid sheaths to come out to be broken off to obtain algal colonization segments with gas vesicles to be uniformly dispersed in aninduction culture solution, the algal colonization segment is uniformly dispersed in the induction culture solution, such that the algal colonization segment induction period is substantially shortened, the mechanical damage is extremely low, the nutrition and the illumination required by the algal colonization segment development are provided during the induction process, and the algal colonization segment development activity is effectively enhanced.

Description

technical field [0001] The invention relates to the technical field of cultivating Gexianmi, in particular to a method for inducing algal growth of Gexianmi. Background technique [0002] Gexianmi is a freshwater nitrogen-fixing cyanobacteria, which is one of the typical Nostoc algae. Algae reproduction is the main reproduction mode of Nostoc algae. A fast and rapid algae breeding technology is the nuclear technology of the large-scale cultivation of Gexianmi, and the core of the algae breeding technology is to have a fast, high activity, and large number of algae breeding segment induction method. In the breeding technology of Gexianmi, the microspheres of Gexianmi microspheres in which the internal algae filaments are distributed in a filamentous form are respectively used as the starting point to breed the microspheres of Gexianmi in the algae propagation section and the homogenized algae filaments. It has superiority, which is characterized by rapid development of alga...

AI Technical Summary

Problems solved by technology

This method is complicated to operate, requires antibiotics to be aseptically purified, and the algae preparation cycle is long, and the number of algae growth stages is relatively small, which is difficult to adapt to the needs of large-scale breeding of algae

The existing patent (application number: 201410269492.4) soaks the kudzu spheres in purified water and induces light source. After the kudzu spheres burst spontaneously, the filtrate is collected to obtain the algae growth section. Although the induction effect of the algae growth section is good, However, the driving force for the bursting of Gexianmi is nutrient starvation. Usually, organisms have a certain tolerance period for starvation. Using nutrient starvation as the power to induce the algal growth of Gexianmi has a non-negligible impact on the induction efficiency of algal growth. The duration is longer; moreover, the static induction, the viscous colloids rich in Gexianmi make the algae grow in clusters and accumulate and the dispersion is poor; the general way for the algae to maintain activity is to absorb nutrients from the environment and start to develop. However, the purified water is deficient in nutrients and cannot meet the nutritional needs of the development of the algae growth stage, resulting in low activity of the algae growth stage

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