Pepper phytophthora blight control method

Abstract

The invention discloses a pepper phytophthora blight prevention and control method, which belongs to the technical field of biology, and comprises the following steps: when pepper seedlings are transplanted and when a first pepper phytophthora blight plant appears, a compound microbial agent is used for irrigating roots of pepper plants; the volume ratio of the paecilomyces lilacinus suspension to the bacillus mojavensis suspension to the penicillium expansum suspension is (3-5): (1-2): 1, the effective viable count of the paecilomyces lilacinus suspension is 2 * 10 < 8 > CFU / mL, and the effective viable count of the bacillus mojavensis suspension and the effective viable count of the penicillium expansum suspension are both 1 * 10 < 8 > CFU / mL. The prevention and treatment method can effectively prevent and treat pepper phytophthora blight.

Description

Technical Field

[0001] The present invention belongs to the technical field of vegetable disease control, and specifically relates to a method for controlling Phytophthora blight of pepper. Background Art

[0002] Pepper (Capsicum annum L.) is one of the vegetable crops with the largest planting area in China. With the increasing annual planting area of protected vegetables in China, Phytophthora blight of pepper has become a devastating soil-borne disease that seriously affects the yield and quality of pepper. Its pathogen, Phytophthora capsici, has a wide range of hosts and can survive in the soil for a long time. Once introduced, it is difficult to cure. In severe cases, it can cause the destruction of greenhouses, resulting in huge economic losses and seriously threatening the sustainable development of the pepper industry in China.

[0003] Using pesticides to control Phytophthora blight of pepper will not only damage the environment but also cause pesticide residue problems. Currently, biological control is the most environmentally friendly control method. Summary of the Invention

[0004] In order to overcome the problems existing in the prior art, the present invention provides a astaxanthin soft capsule and its preparation process. By optimizing the excipients and capsule wall formula, the present invention improves the stability and bioavailability of astaxanthin in capsules.

[0005] To achieve the above object, the present invention provides a method for controlling Phytophthora blight of pepper, comprising the following steps:

[0006] When transplanting pepper seedlings and when the first Phytophthora blight plant appears, use a compound microbial agent to irrigate the roots of pepper plants; the compound microbial agent includes a Paecilomyces lilacinus suspension, a Bacillus mojavensis suspension, and a Penicillium expansum suspension with a volume ratio of 3-5:1-2:1. Among them, the effective viable count of the Paecilomyces lilacinus suspension is: 2×10 8 CFU / mL, and the effective viable counts of the Bacillus mojavensis suspension and the Penicillium expansum suspension are both: 1×10 8 CFU / mL..

[0007] Further, when irrigating the roots of pepper, the dosage of the compound microbial agent is 200-400 mL / plant.

[0008] Further, when the first Phytophthora blight plant appears, irrigate the roots continuously for 2-3 times at intervals of 7 days.

[0009] Through the above technical solutions, the present invention can at least achieve the following beneficial effects: The present invention can effectively control Phytophthora blight of pepper. Detailed Embodiments

[0010] Unless otherwise specified, the materials and reagents used in the present invention are commercially available.

[0011] In the present invention, Paecilomyces lilacinus, Bacillus mojavensis and Penicillium expansum are all purchased from the Engineering Research Center of Industrial Microbial Strains; among them, the number of Paecilomyces lilacinus is: BNCC342068, the number of Penicillium expansum is: BNCC146144, and the number of Bacillus mojavensis is: BNCC358077.

[0012] In the present invention, the preparation method of the Paecilomyces lilacinus and Penicillium expansum bacterial suspensions is as follows: inoculate Paecilomyces lilacinus or Penicillium expansum into a liquid medium (sodium nitrate: 3.0 g, dipotassium hydrogen phosphate: 1.0 g, magnesium sulfate: 0.5 g, potassium chloride: 0.5 g, ferrous sulfate: 0.01 g, sucrose: 30.0 g, water 1000 ml) for fermentation culture at 25°C - 28°C, aerobic, and ferment for 2 - 3 days to obtain the Paecilomyces lilacinus or Penicillium expansum bacterial suspension.

[0013] In the present invention, the preparation method of the Bacillus mojavensis bacterial suspension is as follows: inoculate the purchased test tube strain of Bacillus mojavensis into NB liquid medium for culture, and culture it on a shaker at a temperature of 25 - 28°C and a rotation speed of 200 - 220 r / min for 30 - 35 h to obtain a liquid strain.

[0014] Inoculate the liquid strain of Bacillus mojavensis into a liquid fermentation medium (95 g of potato, 80 g of corn starch, 5 g of peptone, 12 g of ammonium chloride, 10 g of sucrose, water 1000 ml; pH = 7.2 - 7.5) for fermentation culture according to a volume ratio of 0.1:1. The fermentation culture conditions are: 26°C - 28°C, dissolved oxygen 60% - 70%, stirring 180 - 200 r / min, and culture for 36 - 42 h to obtain the Bacillus mojavensis bacterial suspension.

[0015] Example 1

[0016] When transplanting pepper seedlings and when the first pepper blight plant appears, use the compound microbial agent to irrigate the roots of pepper plants; the compound microbial agent includes Paecilomyces lilacinus bacterial suspension, Bacillus mojavensis bacterial suspension and Penicillium expansum bacterial suspension with a volume ratio of 4:1.5:1. Among them, the effective viable count of the Paecilomyces lilacinus bacterial suspension is: 2×10 8 CFU / mL, and the effective viable counts of both the Bacillus mojavensis bacterial suspension and the Penicillium expansum bacterial suspension are: 1×10 8 CFU / mL.

[0017] Among them, when irrigating the roots of peppers, the usage amount of the compound microbial agent is 300 mL / plant.

[0018] Among them, when the first pepper blight plant appears, irrigate the roots continuously for 3 times at an interval of 7 days.

[0019] Example 2

[0020] When transplanting pepper seedlings and when the first pepper phytophthora plant appears, use a compound microbial agent to irrigate the roots of pepper plants; the compound microbial agent includes a Paecilomyces lilacinus suspension, a Bacillus mojavensis suspension, and a Penicillium expansum suspension with a volume ratio of 3:1:1. Among them, the effective viable count of the Paecilomyces lilacinus suspension is: 2×10 8 CFU / mL, and the effective viable counts of the Bacillus mojavensis suspension and the Penicillium expansum suspension are both: 1×10 8 CFU / mL.

[0021] Among them, when irrigating the roots of peppers, the usage amount of the compound microbial agent is 200 mL / plant.

[0022] Among them, when the first pepper phytophthora plant appears, irrigate the roots continuously for 2 times, with an interval of 7 days.

[0023] Example 3

[0024] When transplanting pepper seedlings and when the first pepper phytophthora plant appears, use a compound microbial agent to irrigate the roots of pepper plants; the compound microbial agent includes a Paecilomyces lilacinus suspension, a Bacillus mojavensis suspension, and a Penicillium expansum suspension with a volume ratio of 5:2:1. Among them, the effective viable count of the Paecilomyces lilacinus suspension is: 2×10 8 CFU / mL, and the effective viable counts of the Bacillus mojavensis suspension and the Penicillium expansum suspension are both: 1×10 8 CFU / mL.

[0025] Among them, when irrigating the roots of peppers, the usage amount of the compound microbial agent is 400 mL / plant.

[0026] Among them, when the first pepper phytophthora plant appears, irrigate the roots continuously for 3 times, with an interval of 7 days.

[0027] Comparative Example 1

[0028] The difference between Comparative Example 1 and Example 1 is that the compound microbial agent does not contain the Paecilomyces lilacinus suspension.

[0029] Comparative Example 2

[0030] The difference between Comparative Example 2 and Example 1 is that the compound microbial agent does not contain the Bacillus mojavensis suspension.

[0031] Comparative Example 3

[0032] The difference between Comparative Example 3 and Example 1 is that the compound microbial agent does not contain the Penicillium expansum suspension.

[0033] Pot experiment

[0034] Select seedlings of pepper plants with consistent growth and no obvious pests and diseases for potting and transplanting experiments. According to the compound microbial inoculants described in Examples 1 to 3 and Comparative Examples 1 to 3, irrigate the roots of the transplanted pepper plants at 300 mL per plant; and set up a blank control group without irrigating the compound microbial inoculant. 7 days after the compound microbial inoculant is irrigated to the roots, use the pathogen Phytophthora capsici bacterial suspension (effective viable count 1×10 8 CFU / mL) to irrigate the roots of the pepper plants in the experimental group and the blank control group, 200 mL per plant. After 15 days of irrigating the pathogen, count the incidence of Phytophthora blight in the pepper plants and calculate the incidence rate. The statistical results are shown in Table 1 below:

[0035] Incidence rate = (number of diseased plants / total number of transplanted plants) * 100%;

[0036] Table 1 Incidence of pepper plants in potting experiments

[0037]

[0038]

[0039] It can be seen from Table 1 above that the control method described in the present invention can effectively control Phytophthora blight of peppers.

[0040] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A method for preventing and controlling pepper blight, characterized in that: The following steps are included: When pepper seedlings are transplanted and when the first pepper blight plant appears, a composite microbial agent is used to irrigate the pepper plants; the composite microbial agent comprises a suspension of Paecilomyces lilacinus, a suspension of Bacillus mohaivii and a suspension of Penicillium expansum in a volume ratio of 3-5:1-2:1, wherein the effective viable count of the suspension of Paecilomyces lilacinus is: 2×10 8 CFU / mL, the effective viable counts of Bacillus mohaiwei suspension and Penicillium expansum suspension were both: 1×10 8 CFU / mL.

2. A pepper blight control method according to claim 1, characterized in that: When irrigating the roots of peppers, the dosage of the composite microbial agent is 200-400mL / plant.

3. A pepper blight control method according to claim 1, characterized in that: When the first pepper blight plant appears, irrigate the roots 2-3 times continuously with an interval of 7 days.

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