Secreted Insecticidal Protein and Gene Compositions from Bacillus Thuringiensis and Uses Therefor

a technology of insecticidal protein and bacillus thuringiensis, which is applied in the field of new family of genes encoding lepidopterantoxic proteins and insecticidal fragments, can solve the problems of high toxicity of crystal proteins to specific insects, all field crops, plants, commercial farming areas are susceptible to attack, etc., and achieve effective control of a particular insect pest species, reduce the likelihood of resistance to the insecticidal composition, and improve the effect of insect resistance managemen
US20080040827A1Inactive Publication Date: 2008-02-14DONOVAN JUDITH +4
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
DONOVAN JUDITH
Publication Date
2008-02-14
Estimated Expiration
Not applicable · inactive patent
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Abstract

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
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Description

BACKGROUND OF INVENTION

[0001] The present invention relates to a new family of genes encoding lepidopteran-toxic proteins and insecticidal fragments thereof. In particular, the present invention is directed to exemplary proteins designated herein as TIC900, TIC402, TIC403, TIC404, TIC961, TIC962, TIC963, TIC965 and TIC966, and insecticidal fragments thereof, each encoded by exemplary nucleotide coding sequences designated herein respectively as tic900, tic402, tic403, tic404, tic434, tic961, tic962, tic963, tic965, and tic966, as well as to nucleotide sequence homologs that (1) encode insecticidal proteins and (2) hybridize to the tic900, tic402, tic403, tic404, tic434, tic961, tic962, tic963, tic965, and tic966 coding sequences under stringent hybridization conditions. The present invention also relates to host cells transformed with one or more nucleotide sequences of the present invention or transformed with variants of the nucleotide sequences set forth herein, genes related by i...

Claims

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