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Fast screening method of drought-tolerant rice

A screening method and rice technology, applied in germination equipment and other directions, can solve the problems of different testing time and evaluation methods, inconsistent conclusions, etc., and achieve the effect of effective and rapid screening, good accuracy and simple operation.

Inactive Publication Date: 2012-06-20
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the indoor simulated drought condition method is a commonly used method for many years. Its advantages are suitable for early identification, easy control of conditions, good repeatability, simple method, suitable for large-scale identification, and short test period. The test time and evaluation methods are different, and the conclusions are not consistent

Method used

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  • Fast screening method of drought-tolerant rice
  • Fast screening method of drought-tolerant rice
  • Fast screening method of drought-tolerant rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the rapid screening method of drought-tolerant functional rice, comprising the following steps:

[0035] (1) Disinfection treatment of rice seeds

[0036] Take 380-420 complete and plump rice seeds with different drought resistance properties to be tested and put them into different culture bottles, and then use 1-1.5mol / L HNO 3 After soaking for 28-32min, wash 3-4 times with distilled water, and then wash with 8-12% H 2 o 2 Treat for 10-20min, then wash with distilled water for 3-4 times, dry and set aside;

[0037] (2) Preparation of high-concentration PEG permeate

[0038] Accurately weigh PEG with a molecular weight between 6000-20000, and prepare it with distilled water. The concentration is controlled between 18%-23%. Taking the total volume of 30ml as an example, the proportioning ratio is shown in Table 1.

[0039]

[0040] (3) Germination test of rice seeds

[0041] Spread 2-3 layers of filter paper on each petri dish, then put 28-32ml of ...

Embodiment 2

[0044] Embodiment 2: the present invention decides specific operation process:

[0045] 1. Disinfection treatment of rice seeds:

[0046] Different rice materials (IIyou 838, Huyou 2, Huhan 15, Luhan 1, Zaoxian 15, Kyou 52, Xieyou 52, Huhan 15, Chaofengzao, Plant protection upland rice, Huhan No. 3, Huhan No. 1, transgenic 5, transgenic 2, transgenic 3) 400 grains of mature and plump seeds without insect erosion were put into mesh bags, soaked in 1mol / LHNO3 for 30min, washed with distilled water for 3 times, and then with 10% H 2 o 2 Treat for 10min-20min, then wash 3 times with distilled water, dry and set aside;

[0047] 2. Preparation of high-concentration PEG permeate

[0048] Take PEG8000, 20% concentration, 30ml as an example, accurately weigh 6g of PEG8000, then add 24ml of distilled water to fully dissolve, other molecular weight PEGs and different concentrations can be carried out as shown in Table 1.

[0049] 3. Germination test of rice seeds

[0050] Taking PE...

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Abstract

The invention discloses a fast screening method of drought-tolerant rice. The method can fast screen drought-tolerant rice by simulating drought stress conditions of root growth and germination of seeds by using a high-concentration high molecular polyethylene glycol penetrating fluid and determining the rooting rate and root-bud ratio of rice seeds in the highly penetrating fluid. The method is simple and rapid to operate, has good accuracy and achieves effective and fast screening of drought-tolerant rice.

Description

technical field [0001] The invention relates to a rice screening method, in particular to a rapid screening method for drought-tolerant rice. Background technique [0002] China is a country that is very short of water resources and is listed as one of the 13 water-poor countries by the United Nations. The sustainable development of China's agriculture is facing a serious water crisis. In the 1950s, the average annual drought-affected area in the country was 11.33 million hm2, and it increased to 23.33 million hm2 and 26.67 million hm2 respectively in the 1980s and 1990s. The irrigated area of ​​6.67 million hm2 cannot be irrigated, and the grain production has been reduced by 70 billion to 80 billion kg due to water shortage. Drought has long been one of the main obstacles affecting rice production. Therefore, it has become an important issue for breeders to accurately identify the drought resistance of rice germplasm and to breed rice varieties with strong drought resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 张瑛罗志祥施伏芝阮新民滕斌吴敬德周俊峰
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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