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Application of waste compost composite bacterial agent to improving drought resistance of lawn grass

A technology of compound bacterial agent and drought resistance, applied in the fields of application, preparation of organic fertilizer, bacteria, etc., can solve the problems of plant growth and physiological characteristics that have not been reported

Inactive Publication Date: 2011-09-07
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in any case, in the process of compound microbial inoculum research, related studies on the effects of composting microbial inoculants on plant growth and physiological characteristics have not been reported.

Method used

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  • Application of waste compost composite bacterial agent to improving drought resistance of lawn grass
  • Application of waste compost composite bacterial agent to improving drought resistance of lawn grass
  • Application of waste compost composite bacterial agent to improving drought resistance of lawn grass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] (1) Screening method of strains

[0067] Enrichment: Weigh 10g of the collected compost sample and place it in a sterile Erlenmeyer flask, add 100ml of sterile water and glass beads and shake evenly, then take 10ml of the suspension in a Erlenmeyer flask filled with 100ml of enrichment medium, respectively Cultivate for 3 days on a shaker shaker at a suitable temperature.

[0068] (2) Preliminary screening: After diluting the enriched culture solution in a concentration gradient, spread it on the separation and screening medium, culture it upside down at a constant temperature, observe the size of the transparent circle, and select the colony with a large ratio of transparent circle / colony diameter Perform re-screening.

[0069] (3) Re-screening: inoculate the strains obtained from the initial screening into the corresponding shake flask culture medium, and cultivate them under the conditions of suitable temperature and shaking table speed. The OD value is the ordinate, ...

Embodiment 2

[0076] The perennial ryegrass ( Lolium perenne L.) and tall fescue ( Festuca arundinacea L.) is the test material. The seeds of the tested turfgrass plants were plump and uniform in size. The soil used is the garden soil of Tianjin Normal University. Its physical and chemical properties are: pH 7.44, saturated water content 0.58mL / g, organic matter 4.68%, total nitrogen 0.21%, available phosphorus 22.03 mg / kg, and total potassium 45.61g / kg. After collection, remove plant residues, gravel and other sundries, pass through a 2mm sieve, and sterilize at 121°C for 30 minutes for later use. The method of potting was adopted in the experiment, 200 g of sterilized soil was placed in each culture container, and 0.4 g of lawn seeds were sown on it. Provide uniform and quantitative water every day to maintain a good water condition in the culture medium, and change the position of each culture container frequently to ensure consistent light. When the plant grows to a height of 7 cm...

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Abstract

The invention discloses application of a municipal domestic waste compost composite bacterial agent to improving drought resistance of lawn grass. The compost composite bacterial agent consists of liquid which is formed by diluting bacillus subtilis, actinomycetes and saccharomycetes in a volume ratio of 1 to 1 to 1 by 100 to 200 times. A result shows that drought damage of plants can be relieved to maintain a normal physiological metabolism function of the plants through activity of protective enzyme and osmotic adjustment substance content after diluted municipal domestic waste compost composite microbial bacterial agent is inoculated in the plants, so that the damage to the lawn plants caused by drought stress is effectively relieved, the drought resistance of the lawn plants is improved, and a basis is provided for planting of the lawn plants under a drought environment.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to the preparation of a high-efficiency composting compound bacterial agent by using beneficial bacteria extracted from urban domestic garbage composting as raw materials. More specifically, it is the application of the compound bacterial agent in preparing and improving the drought resistance of lawn plants. Background technique [0002] Composting is a biochemical process that uses microorganisms (bacteria, actinomycetes, fungi, etc.) widely present in nature or commercial strains to controlly promote the transformation of biodegradable organic matter into stable humus (humic substance, HS). . This process is also part of the ecological process on the earth's surface, which participates in the cycle of matter and energy on the earth's surface. [0003] Under artificial control conditions, the organic matter in solid waste can be converted into organic fertilizer (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/16C12N1/20C05F11/08C05F17/00C12R1/125C12R1/01C12R1/645
CPCY02W30/40
Inventor 多立安赵树兰王晶晶
Owner TIANJIN NORMAL UNIVERSITY
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