Application of waste compost composite bacterial agent to improving drought resistance of lawn grass
A technology of compound bacterial agent and drought resistance, applied in the fields of application, preparation of organic fertilizer, bacteria, etc., can solve the problems of plant growth and physiological characteristics that have not been reported
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Embodiment 1
[0066] (1) Screening method of strains
[0067] Enrichment: Weigh 10g of the collected compost sample and place it in a sterile Erlenmeyer flask, add 100ml of sterile water and glass beads and shake evenly, then take 10ml of the suspension in a Erlenmeyer flask filled with 100ml of enrichment medium, respectively Cultivate for 3 days on a shaker shaker at a suitable temperature.
[0068] (2) Preliminary screening: After diluting the enriched culture solution in a concentration gradient, spread it on the separation and screening medium, culture it upside down at a constant temperature, observe the size of the transparent circle, and select the colony with a large ratio of transparent circle / colony diameter Perform re-screening.
[0069] (3) Re-screening: inoculate the strains obtained from the initial screening into the corresponding shake flask culture medium, and cultivate them under the conditions of suitable temperature and shaking table speed. The OD value is the ordinate, ...
Embodiment 2
[0076] The perennial ryegrass ( Lolium perenne L.) and tall fescue ( Festuca arundinacea L.) is the test material. The seeds of the tested turfgrass plants were plump and uniform in size. The soil used is the garden soil of Tianjin Normal University. Its physical and chemical properties are: pH 7.44, saturated water content 0.58mL / g, organic matter 4.68%, total nitrogen 0.21%, available phosphorus 22.03 mg / kg, and total potassium 45.61g / kg. After collection, remove plant residues, gravel and other sundries, pass through a 2mm sieve, and sterilize at 121°C for 30 minutes for later use. The method of potting was adopted in the experiment, 200 g of sterilized soil was placed in each culture container, and 0.4 g of lawn seeds were sown on it. Provide uniform and quantitative water every day to maintain a good water condition in the culture medium, and change the position of each culture container frequently to ensure consistent light. When the plant grows to a height of 7 cm...
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