A method for improving the drought resistance of turfgrass by using drought-resistant intensified active nano-waste compost
A technology of nano-garbage composting and lawn grass, applied in the field of environmental protection
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Embodiment 1
[0094] A method for improving the drought resistance of turfgrass by using drought-resistant enhanced active nano-garbage composting, characterized in that it is carried out according to the following steps:
[0095] (1) Preparation of nano-compost: Dry the domestic waste compost at 105°C to constant weight, process and grind the waste compost to make 30-35nm nano-compost for later use;
[0096] (2) Preparation of drought-tolerant microbial inoculum
[0097] Weigh 10g of the compost sample and place it in a sterile Erlenmeyer flask, add 100mL of sterile water and shake it evenly, then take 10mL of the suspension in a Erlenmeyer flask containing 100mL of enrichment medium, at 28°C, 220r / min Shaking culture for 3 days is the mixed microbial flora; the drought tolerance enhancement of the mixed microbial flora adopts the method of gradually increasing the concentration of PEG6000. During the strengthening process, depending on the OD 600 Increase and gradually increase the conce...
Embodiment 2
[0111] The method of improving the drought resistance of turfgrass by using drought-resistant enhanced active nano-garbage composting:
[0112] (1) Preparation of nano-compost: Dry the domestic waste compost at 105°C to constant weight, process and grind the waste compost to make 30-35nm nano-compost for later use;
[0113] (2) Preparation of drought-tolerant microbial inoculum
[0114] Weigh 10g of the compost sample and place it in a sterile Erlenmeyer flask, add 100mL of sterile water and oscillate evenly, then take 10mL of the suspension in a Erlenmeyer flask filled with 100mL of enrichment medium, at 28°C, 220r / min Shaking culture for 3 days is the mixed microbial flora; the drought tolerance enhancement of the mixed microbial flora adopts the method of gradually increasing the concentration of PEG6000. During the strengthening process, depending on the OD 600 Increase and gradually increase the concentration of PEG6000, the concentration of PEG6000 increases with a grad...
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