Method for adjusting drought-stressed turfgrass protective enzymes by using drought-resistant enhanced active nano-garbage compost
A technology of nano-garbage composting and drought stress applied in the field of environmental protection
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[0089] A method for regulating drought-stressed lawn grass protective enzymes by adopting drought-tolerant intensified active nano garbage compost:
[0090] (1) Preparation of nano-compost: dry the domestic waste compost at 105°C to a constant weight, and process and grind the waste compost into 30-35nm nano-compost, which is ready for use;
[0091] (2) Preparation of drought-tolerant microbial agents
[0092] Weigh 10g of the compost sample and place it in a sterile Erlenmeyer flask, add 100mL sterile water and shake it evenly, then take 10mL of the suspension in an Erlenmeyer flask containing 100mL enriched medium, at 28℃, 220r / min Shake culture for 3 days, that is, the mixed microbial flora; the drought tolerance of the mixed microbial flora is enhanced by gradually increasing the concentration of PEG6000. During the strengthening process, depending on the OD 600 Increase and gradually increase the concentration of PEG6000. The concentration of PEG6000 increases with a 5% gradient...
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