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Method for efficiently selecting drought-resisting alfalfa variety

A kind of technology of alfalfa and varieties, which is applied in the fields of botany equipment and methods, horticulture, plant cultivation, etc. It can solve the problems that it is difficult to accurately screen out drought-tolerant alfalfa varieties, and does not consider the different sensitive physiological index systems.

Inactive Publication Date: 2017-05-31
GANSU AGRI UNIV
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Problems solved by technology

[0004] Although the above techniques have screened drought-resistant alfalfa varieties by PEG simulating drought stress combined with measuring some physiological indicators, and at the same time determined the key water potential points of alfalfa seedlings for drought resistance and the physiological indicators related to alfalfa drought resistance, but the above screening methods did not consider different PEGs. Under concentration stress, the sensitive physiological index system of alfalfa varieties with different drought resistance ability is different, and there is also a lack of tolerance among alfalfa varieties with different drought resistance levels under extreme drought stress conditions, as well as the screening of sensitive physiological indicators reflecting the strong drought resistance of alfalfa varieties. Therefore, it is difficult to accurately screen out alfalfa varieties with strong drought tolerance

Method used

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Embodiment 1

[0021] A method for efficiently screening drought-resistant alfalfa varieties, the steps are as follows:

[0022] (1) Cultivation of alfalfa seedlings. Any method for cultivating alfalfa seedlings in the prior art can be used for culturing, such as sand culture with nutrient solution. Sow the tested alfalfa seeds in the nutrient pot, thin out the seedlings after emergence, and water the Hoagland nutrient solution during the growth period;

[0023] (2) The Hoagland nutrient solution containing 0.38 g / mL (-1.6 MPa) and 0.43 g / mL (-2.0 MPa) PEG-6000 solution was used to simulate drought stress, and the rhizosphere stress of alfalfa seedlings growing for about 6 weeks was treated for 7 days;

[0024] (3) Measure the following physiological indicators of alfalfa under the stress of two PEG-6000 concentrations;

[0025] 1) Under the stress of 0.38 g / mL PEG-6000 solution, the hydroxyl radical (OH • ) concentration and catalase (CAT) activity;

[0026] 2) Under 0.43g / mL PEG-6000 s...

Embodiment 2

[0032] In this experiment, the strong drought-resistant alfalfa variety Longzhong was grown in the arid area of ​​the Loess Plateau with an annual rainfall of 200-350 mm, annual evaporation of 1531 mm, annual average temperature of 6.3 °C, annual sunshine hours of 2500 h, and an average frost-free period of 140 days. Taking alfalfa as an example, PEG-6000 is used to simulate drought stress, and its physiological indicators that are more sensitive to drought stress are measured. The steps are as follows:

[0033] (1) Longzhong alfalfa seedling cultivation. Using nutrient solution sand culture method. Sow the tested alfalfa seeds in the nutrient pot, thin out the seedlings after emergence, and water the Hoagland nutrient solution during the growth period;

[0034](2) The Hoagland nutrient solution containing 0.38 g / mL (-1.6 MPa) and 0.43 g / mL (-2.0 MPa) PEG-6000 solution was used to simulate drought stress, and the rhizosphere stress of alfalfa seedlings growing for about 6 wee...

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Abstract

The invention provides a method for efficiently selecting drought-resisting alfalfa variety. The method includes steps of (1), seedling cultivation of alfalfa; (2), simulating drought stress by nutrition liquid containing 0.38 g / mL and 0.43 g / mL PEG-6000 solution, and performing the drought stress on the alfalfa seedling root grown for 40-45 days; (3), respectively testing the following physical signs of the alfalfa under the stress of two PEG-6000 concentrations; (4), according to sensitive threshold values of every geological index under the stress of PEG-6000 solutions with different concentrations, and selecting the drought-resisting alfalfa variety. Two PEG-6000 solutions with greatly high concentrations are applied to measure the alfalfa physical signs greatly contributed to the drought resistance by simulating the strong drought stress environments; through comparing the endurance capacity of different types of drought-resisting alfalfa to high-concentration PEG stress and corresponding change threshold value of sensitive physical signs, the drought-resisting alfalfa variety can be exactly and efficiently screened.

Description

technical field [0001] The invention relates to a method for efficiently screening drought-resistant alfalfa varieties. Background technique [0002] Screening alfalfa varieties with strong drought resistance is of great significance for further mining and utilization of excellent drought-resistant alfalfa germplasm resources. In the past, the commonly used screening methods for drought-resistant alfalfa varieties were mostly by measuring field indicators or combining several physiological indicators, and using some recognized evaluation methods such as direct comparison method, total drought resistance evaluation method, membership function method, analytic hierarchy process (AHP), main Component analysis and gray relational degree model were used to classify alfalfa varieties with different levels of drought resistance, and some drought-resistant alfalfa varieties were screened out. At present, there are many and inconsistent evaluation methods for the drought resistance ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
CPCA01G22/00
Inventor 师尚礼张翠梅尹国丽王宝文刘珍杨帆
Owner GANSU AGRI UNIV
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