The utility model relates to the technical field of bolts, and discloses a bolt thread tolerance testing fixture, which comprises a base, a sliding chute arranged on the lower surface of the base, a fixing piece arranged on the upper surface of the base, a bolt sleeve arranged on one side of the fixing piece, a sliding piece arranged on the upper surface of the base, and a supporting piece arranged on the upper surface of the sliding piece. A rotating piece is installed on one side of the supporting piece, a connecting piece is installed on one side of the rotating piece, and a thread ring gauge is installed on one side of the connecting piece. Through the first sliding block, the second sliding block and the sliding groove, the moving track of the bolt is enhanced, errors in the measuring process are reduced, the rotating piece can rotate flexibly through the balls, the thread ring gauge can rotate forwards and reversely, the position of the thread ring gauge can be adjusted conveniently, and through the first annular clamping block and the fixing piece, bolt sleeves of different models can be replaced conveniently. Measurement requirements of bolts of different models are met, the connecting piece is connected with the thread ring gauge in a sleeved mode, replacement of the thread ring gauges of different models is facilitated, and measurement efficiency is improved.