2 results about "Thiobacillus denitrificans" patented technology

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.

This invention relates to the field of environmental microbiology, specifically disclosing an autotrophic denitrifying bacterial agent and its preparation method. This autotrophic denitrifying bacterial agent employs a three-layer biomimetic design: a sulfur-calcium composite core, an inner shell gel layer, and an outer functional layer. Denitrifying Thiobacillus is loaded into the core, and Pseudomonas schlegelii is fixed in the outer layer, with a sodium alginate-xanthan gum gel layer providing physical isolation, achieving spatial partitioning and symbiosis of autotrophic and heterotrophic denitrifying bacterial communities. The sulfur-calcium composite core uses sulfur powder as an electron donor, and hydrophobically modified eggshell powder supplements alkalinity in situ. The inner shell layer incorporates ultrafine calcium carbonate, providing both mechanical reinforcement and pH buffering functions. The outer layer encapsulates sodium acetate-stearate microspheres for slow carbon source release. This autotrophic denitrifying bacterial agent exhibits high denitrification efficiency, pH stability, and strong resistance to hydraulic shearing under low carbon-to-nitrogen ratio conditions. The preparation process is mild, and the raw materials are inexpensive and readily available, making it suitable for deep denitrification in wastewater treatment and other scenarios.