25 results about "Thiobacillus denitrificans" patented technology

The present invention provides a sulfur autotrophic denitrification filler and a preparation method thereof. The sulfur autotrophic denitrification filler of the present invention comprises the following raw materials in parts by weight: 50-100 parts of sulfur powder, 5-20 parts of pH buffer, 10-50 parts of medical stone, and 0-10 parts of additives; wherein the pH buffer is one or more of sodium carbonate, sodium bicarbonate, sodium hydroxide, and potassium hydroxide; and the additive is one or more of amide substances and epoxy resins. The sulfur autotrophic denitrification filler has good filler strength and a long service life, and can be conducive to the attachment of denitrifying thiobacillus to the surface of the sulfur autotrophic denitrification filler, thereby increasing the microbial concentration per unit volume of the filler and increasing the reaction rate.

The invention discloses a method for reinforcing denitrification of a sulfur circulation microbial system by using an iron carrier, and belongs to the technical field of nitrogen-containing wastewater treatment. The method comprises the following steps: inoculating sulfate reducing bacteria and thiobacillus denitrificans into a culture medium containing iron ions or ferrous ions for domestication; and applying the domesticated complex microbial inoculant to wastewater denitrification. In a sulfur circulating microorganism system, Fe < 2 + > can promote the activity of a biological denitrification key enzyme in a biological system by forming an iron carrier in a form of FeS, S < 2-> is fixed in the system and prevented from escaping, and when electron donors in the system are insufficient, the Fe < 2 + > can be used as a substitute electron donor for biological denitrification, so that the requirement on an external carbon source is reduced, and the energy consumption is reduced. And meanwhile, the impact load resistance of the system can be enhanced. Through sulfate reduction and sulfur autotrophic denitrification and iron coupling, sulfate in wastewater is converted into electron donors, a carbon-nitrogen-sulfur-iron biological co-reaction system is formed, the defects of a single-strain denitrification system are overcome, the denitrification effect is enhanced, and energy consumption and cost are reduced.

The invention relates to the technical field of environmental microorganisms and sewage treatment, and provides a cold-resistant sulfur autotrophic denitrifying bacterium and application thereof, the sulfur autotrophic denitrifying bacterium is thiobacillus denitrificans SZG-SAD-004, and the preservation number of the sulfur autotrophic denitrifying bacterium is CCTCC (China Center for Type Culture Collection) NO: M2023992. The Thiobacillus denitrificans SZG-SAD-004 disclosed by the invention can help a sulfur autotrophic denitrification device to be quickly started at a low temperature, the normal-temperature starting efficiency is achieved, meanwhile, efficient denitrification can still be performed at the temperature of 6-15 DEG C, the low-temperature tolerance performance and the denitrification performance are strong, and the Thiobacillus denitrificans SZG-SAD-004 has a very high application value.

The invention discloses a short-cut nitrification-iron coupling sulfur cycle biological denitrification method, and belongs to the technical field of nitrogen-containing wastewater treatment. According to the method, ammonia nitrogen in the wastewater is converted into nitrite by utilizing a domesticated nitrosobacteria agent; then, sulfate reducing bacteria and thiobacillus denitrificans are inoculated into a culture medium containing iron ions or ferrous ions together for domestication to obtain a complex microbial inoculant, and nitrite and nitrate in the wastewater are further removed through the complex microbial inoculant. In a sulfur circulating microorganism system, Fe < 2 + > can promote the activity of a biological denitrification key enzyme in a biological system by forming an iron carrier in the form of FeS, S < 2-> is fixed in the system and prevented from escaping, and when electron donors in the system are insufficient, the Fe < 2 + > can be used as a substitute electron donor for biological denitrification, so that the requirement on an external organic carbon source is reduced, and the energy consumption is reduced. And meanwhile, the impact load resistance of the system can be enhanced. Through sulfate reduction and sulfur autotrophic denitrification and iron coupling, sulfate in wastewater is converted into electron donors, a carbon-nitrogen-sulfur-iron biological co-reaction system is formed, the defects of a single-strain denitrification system are overcome, the denitrification effect is enhanced, and energy consumption and cost are reduced.

The invention discloses a sewage denitrification baffled reactor, belonging to the technical field of sewage treatment. A sewage denitrification baffled reactor is mainly composed of an inlet area, a baffled biological denitrification area, an outlet area, a sulfur powder adding device, a sulfur powder recovery device, and a clean water reflux device. When the invention is applied to the secondary biochemical effluent deep denitrification treatment of a sewage treatment plant, the water flow is in a turbulent state, which improves the mass transfer efficiency, enhances the activity of denitrifying thiobacillus, reduces the total nitrogen content in the sewage, and reduces environmental pollution.

The invention relates to the technical field of sewage treatment materials, and particularly discloses a sludge domestication nutrient solution, autotrophic denitrification sludge and a preparation method and application thereof. The sludge domestication nutrient solution provided by the invention contains various nutritional ingredients required by metabolism of different flora microorganisms, so that the problem of weakening of flora variety diversity caused by insufficient nutrient elements is avoided; the sludge domestication nutrient solution is rich in various trace elements and growth promoting factors, promotes the growth activity of various enzymes and microorganisms, also promotes the growth of microbial flora cells, enriches the types and abundance of the flora, and enhances the reproductive capacity and diversity of the flora. According to the method, thiobacillus denitrificans, pseudomonas and lysobacter in sludge are activated into dominant strains by selecting a specific sludge domestication nutrient solution, the dominant strains are applied to sewage, and the dual effects of nitrogen and phosphorus removal are achieved under the condition that no extra carbon source is added.

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.

The present application relates to a kind of sulfur autotrophic denitrification fillings and its preparation method, belong to the sulfur autotrophic denitrification technical field of river and lake ecological management field, including mass ratio 1:1.2:0.2:0.1:0.01 of A component, B component, C component, D component, E component, the A component includes mass ratio 5:3:1:1 of sulfur powder, siderite powder, thiosulfate and active clay;The B component is alginic acid propylene glycol ester aqueous solution;The C component is the mixture of binder and volatile agent with mass ratio 5:2;The D component is hydrogen sulfide remover, neutralizing agent and digestion agent with mass ratio 5:3:2;The E component is denitrifying sulfur bacteria.The present application is a kind of low-cost treatment technology for sulfur autotrophic denitrification, and biological can use sulfate in water to complete denitrification by itself, without the support of external energy, can significantly reduce cost.

This invention relates to the field of environmental microbiology, specifically disclosing an autotrophic denitrifying bacterial agent and its preparation method. This autotrophic denitrifying bacterial agent employs a three-layer biomimetic design: a sulfur-calcium composite core, an inner shell gel layer, and an outer functional layer. Denitrifying Thiobacillus is loaded into the core, and Pseudomonas schlegelii is fixed in the outer layer, with a sodium alginate-xanthan gum gel layer providing physical isolation, achieving spatial partitioning and symbiosis of autotrophic and heterotrophic denitrifying bacterial communities. The sulfur-calcium composite core uses sulfur powder as an electron donor, and hydrophobically modified eggshell powder supplements alkalinity in situ. The inner shell layer incorporates ultrafine calcium carbonate, providing both mechanical reinforcement and pH buffering functions. The outer layer encapsulates sodium acetate-stearate microspheres for slow carbon source release. This autotrophic denitrifying bacterial agent exhibits high denitrification efficiency, pH stability, and strong resistance to hydraulic shearing under low carbon-to-nitrogen ratio conditions. The preparation process is mild, and the raw materials are inexpensive and readily available, making it suitable for deep denitrification in wastewater treatment and other scenarios.

The invention is applicable to the technical field of sewage treatment, and provides a denitrifying sulfur-iron composite filler catalyst and a preparation method thereof, the denitrifying sulfur-iron composite filler catalyst comprises a material A and a material B. The material A is a strain; the material B comprises microporous aggregate, a coating material and a starch layer; the microporous aggregate comprises activated carbon, sponge iron, silicon sulfide and silicon carbide; the coating material comprises a carbon source, a nitrogen source, sulfur powder and iron powder; the mass ratio of the material A to the material B is (1-3): (50-90); preferably, the strain comprises thiobacillus denitrificans, sulfur bacteria and a culture medium, and the content of the thiobacillus denitrificans in the culture medium is (1-5) * 10 < 10 > CFU / ml, and the content of the sulfur bacteria in the culture medium is (0.5-1.5) * 10 < 10 > CFU / ml; the culture medium comprises the following components: 1-2g of ammonium chloride, 2-5g of sodium sulfite, 1-2g of monopotassium phosphate, 0.5-1g of sodium chloride, 0.1-0.2 g of calcium sulfate and 1L of water. The catalyst prepared by the invention is used for sewage treatment, has high denitrification efficiency and certain reusability, and is economical, environment-friendly and low in cost.

The present invention discloses a novel method for treating nitrate in carbon-deficient wastewater using an integrated microbial photoelectrochemical reactor. The method couples TiO2, a nano-semiconductor material with advantages such as stable chemical properties, non-toxicity and high photocatalytic activity, with a Thiobacillus denitrificans biofilm system to form an inorganic semiconductor-microorganism system. Under light conditions, the Thiobacillus denitrificans biofilm uses photogenerated electrons to convert NO3 ‑ This method overcomes the shortcomings of traditional inorganic semiconductor-microorganism integration, such as complex operational processes, reactive oxygen species generated by light that damage bacteria, reduce quantum efficiency, and inhibit nitrous oxide reductase (NOS), resulting in the production of large amounts of greenhouse gases (N2O). It offers the advantages of simple process, low energy consumption, environmental friendliness, economy, safety, and reliability. Furthermore, the system structure is relatively simple, making it easier to modify and improve in a modular manner, thus enabling large-scale promotion and application.

The present invention discloses a denitrifying Thiobacillus mutant strain and a construction method thereof, a bacterial agent and an application thereof, and belongs to the field of microbial technology. The present invention constructs a conjugation transfer system of denitrifying Thiobacillus for the first time, and provides a convenient, rapid and efficient genetic operating system for the creation of engineered denitrifying Thiobacillus based on synthetic biology and its molecular biology research. The strain can be used as a biological denitrifier in the treatment of aquatic animal breeding wastewater, domestic sewage, industrial wastewater and black and odorous water bodies in rivers, and can achieve carbon fixation and emission reduction while removing sulfur and nitrogen from sewage, and green production.

The present invention discloses a microbial remediation agent for water vanadium pollution control and a preparation method thereof, comprising microorganisms and a carrier, wherein the microorganisms include denitrifying Thiobacillus and sulfur-reducing Geobacter, the carrier includes activated carbon, sulfur powder and sodium acetate, and the microbial remediation agent is obtained by loading the microorganisms onto the carrier. The present invention targets V(V) in groundwater, and uses a microbial agent to reduce V(V) in groundwater into V(IV) precipitation, so as to reduce the toxicity and mobility of vanadium in groundwater, and significantly reduce the content of highly toxic V(V). The microbial preparation has high activity, fast reproduction, environmental protection and no pollution, low cost, and reasonable compatibility between denitrifying Thiobacillus and Geobacter, symbiotic coordination, and neither the problem of eutrophication caused by the introduction of a large amount of organic nutrients nor the generation of a large amount of sulfate acidification of groundwater.

The invention discloses a culture medium for rapidly culturing thiobacillus denitrificans as well as a preparation method and application of the culture medium, and belongs to the technical field of microbial culture. Aiming at the chemical energy autotrophic metabolism characteristics of thiobacillus denitrificans, a highly adaptive, stable and efficient growth environment is cooperatively constructed through precise formula regulation and optimized step-by-step preparation and classified sterilization processes. The culture medium disclosed by the invention can remarkably shorten the growth delay phase of thalli, promote the thalli to quickly enter the logarithmic phase and realize high-density and high-activity stable culture, and the cultured thiobacillus denitrificans show excellent denitrification performance in sewage treatment. The culture medium provided by the invention systematically solves the core problems of long culture period, low efficiency, high cost, poor large-scale stability and the like in the prior art, and has a good application prospect.

The invention relates to the technical field of environmental microorganisms, in particular to a normal-temperature preservation method of thiobacillus denitrificans. According to the preservation method of thiobacillus denitrificans, mineral materials such as silica fume and coal gangue are selected and matched with a preservation nutrient solution, a specific metal ion Cu < 2 + > is added to serve as a microbial metabolism regulator, and the synergistic effect of the mineral materials and the preservation nutrient solution is utilized, so that thallus metabolism is moderately inhibited through Cu < 2 + > to reduce substrate consumption, and the toxic effect of Cu < 2 + > on thiobacillus denitrificans is relieved through an adsorption material; the microbial inoculum can be effectively preserved at normal temperature for 3 months, is suitable for preserving a large amount of thiobacillus denitrificans for a long time, and has the advantages of high survival rate, quick activity recovery, low preservation energy consumption and the like.

The invention discloses a thiobacillus denitrificans mutant strain, a construction method of the thiobacillus denitrificans mutant strain, a fungicide and application of the fungicide, and belongs to the technical field of microorganisms. According to the invention, a conjugational transfer system of thiobacillus denitrificans is constructed for the first time, and a convenient, rapid and efficient genetic genetic operating system is provided for engineering thiobacillus denitrificans creation based on synthetic biology and molecular biology research of the engineering thiobacillus denitrificans. The strain can be used as a biological denitrification agent in treatment of aquatic animal breeding wastewater, domestic sewage, industrial wastewater and river black and odorous water, and realizes carbon sequestration, emission reduction and green production while performing desulfurization and denitrification on sewage.

The present application relates to the field of environmental microorganism and sewage treatment technology, and proposes a cold-resistant sulfur autotrophic denitrification bacteria and application thereof, wherein the sulfur autotrophic denitrification bacteria is Thiobacillus denitrificans SZG-SAD-004, and the preservation number is CCTCC NO:M2023992.The Thiobacillus denitrificans SZG-SAD-004 in the present application can help the sulfur autotrophic denitrification device to start quickly at low temperature, achieve normal-temperature starting efficiency, and still achieve high-efficiency denitrification at 6-15 DEG C, and has high application value due to strong low-temperature tolerance and denitrification performance.

The invention belongs to the technical field of water environment treatment, and relates to an in-situ remediation process method for rural black and odorous water, which is suitable for ecological treatment of black and odorous water bottom mud and low-temperature regions. The technology comprises the following steps: screening thiobacillus denitrificans, denitrifying bacteria and phosphorus-accumulating bacteria from the bottom mud of a target water area, preparing a special fungicide combination for three stages of deodorization, denitrification and phosphorus accumulation, and carrying out cross antagonism culture; drying-free agglomeration and wrapping granulation are adopted to prepare double-layer particles; injecting into a bottom mud anaerobic layer through a bottom mud injection device in three stages, and adding corresponding microbial agents according to the stages. Through cold-resistant flora directional reinforcement, double-layer particle controlled release and staged precise addition, the microbial inoculum activity and bottom mud permeability in a low-temperature environment are remarkably improved, the problems of microbial inoculum loss, agglomeration and insufficient pertinence are effectively solved, and efficient ecological management of black and odorous water is realized.

The present invention relates to the technical field of sewage treatment, and in particular to a method for accurately denitrifying sewage based on Thiobacillus denitrificans, comprising a denitrification system consisting of two rows of denitrification units sequentially arranged on a branch water inlet pipe. Fillers are loaded into each denitrification unit and inoculated with Thiobacillus denitrificans, and then sewage is introduced. The super-strong reducing ability of Thiobacillus denitrificans can achieve the reduction and removal of nitrate nitrogen in the sewage. As the operation time increases, the filler layer will inevitably become compacted. At this time, the denitrification units on the two rows of branch water inlets are flipped in turn, so that the compacted and blocked filler layer can be forcibly destroyed, so that the filler layer in the denitrification unit is like a newly loaded one, without the need to replace the filler. At the same time, since the denitrification units are symmetrically arranged on the upper and lower sides, the flipped denitrification units can be used directly, while ensuring the sewage treatment effect, it also effectively reduces the operation and maintenance costs, laying a solid foundation for subsequent promotion.

The invention discloses efficient compound bacteria, which are prepared from the following raw materials in parts by weight: 20 to 25 parts of ammonifying bacteria, 10 to 15 parts of thiobacillus denitrificans, 5 to 10 parts of bacillus licheniformis, 2 to 5 parts of actinomycetes, 2 to 5 parts of saccharomycetes, 3 to 5 parts of rhodopseudomonas palustris, 2 to 5 parts of nitrifying bacteria and 2 to 5 parts of denitrifying bacteria. The microbial agent is a high-efficiency compound microorganism, is in a fine particle shape, generally contains more than 10 < 9 > cfu / g of viable count o (cfu / mL refers to the total number of bacterial communities contained in each milliliter of sample), and is mainly applied to digesting bottom mud, degrading organic matters, eliminating black and odorous substances, inhibiting blue-green algae, adjusting the balance of microbial populations in sewage and complementing background microbial populations.

The invention discloses a high volume load sulfur autotrophic denitrification nitrogen removal process rapid starting method which comprises the following steps: S1, raw material preparation: S11, firstly preparing a thiobacillus denitrificans culture solution and elemental sulfur filler, and filling the lower part of a filter tank with elemental sulfur particle filler; according to the method, thiobacillus denitrificans is adopted as an inoculation source and is matched with the elemental sulfur particle filler, so that a sulfur autotrophic denitrifying bacteria biological membrane can be quickly enriched on the surface of the sulfur-containing filler, the time of domestication by using municipal sludge is effectively shortened, the generation of infectious microbes is reduced to the maximum extent, the volume load of passing through nitrate nitrogen can reach 0.75 kg NO3-N / (m < 3 >. D) or above, and the yield of nitrate nitrogen is increased. The starting time of the sulfur autotrophic denitrification filter tank is 10-20 days, the sulfur autotrophic denitrification filter tank is superior to that of a traditional municipal sludge inoculation domestication method, the high volume load of the sulfur autotrophic denitrification process for treating the landfill leachate is achieved, and the running stability of the sulfur autotrophic denitrification filter tank is improved.

The invention relates to the technical field of applied microorganisms, and discloses a liquid viable bacteria preservation method of thiobacillus denitrificans, which comprises the following steps: inoculating thiobacillus denitrificans into a fermentation culture medium for culture, and carrying out replacement treatment to obtain an original bacteria solution with the sulfur autotrophic denitrification rate of not less than 500mgN.L <-1 >. H <-1 >; after the original bacterial liquid is subjected to centrifugation or membrane concentration, a replacement culture medium is adopted for replacement, the replacement culture medium comprises a compound sulfur source and potassium nitrate, and the compound sulfur source is a combination of a soluble sulfur source and a slow-release sulfur source; and preserving the treated bacterial liquid at a specific temperature, wherein the system is kept in an oxidation state in the preservation process. The liquid viable bacteria preservation method of the thiobacillus denitrificans aims at solving the problem that the thiobacillus denitrificans serving as a liquid fungicide cannot be preserved for a long time at normal temperature.

The invention provides a method for carbon neutralization and synergistic desulfurization and denitrification of industrial flue gas by using microorganisms, which comprises the following steps: carrying out constant-temperature humidifying treatment and biological filtration treatment on the industrial flue gas, and then discharging after reaching the standard, according to the biological filtration treatment, the flue gas subjected to constant-temperature humidification treatment sequentially passes through a plurality of biological filler layers from bottom to top, water leaking from the lower portions of the biological filler layers is sprayed to the biological filler layers, and in the biological filler layer located on the lower side, the water in the biological filler layer is drained; at least one biological filler layer is simultaneously inoculated with a mixed flora of thiobacillus ferrooxidans, sulfate reducing bacteria and thiobacillus ferrooxidans; sulfate reducing bacteria and thiobacillus denitrificans are inoculated in the upper biological filler layer. The high tower with multiple layers of flue gas treatment chambers is adopted for flue gas treatment, the occupied area is small, the pipeline erection cost is saved, meanwhile, multiple biological filtration mechanisms inoculated with specific microorganisms are arranged in the biological filtration tower for flue gas filtration, and carbon emission reduction is achieved while desulfurization and denitrification are conducted.

The present invention is applicable to the technical field of sewage treatment, and provides a denitrifying sulfur-iron composite packing catalyst and a preparation method thereof, which include material A and material B. Material A is a strain; material B includes a microporous aggregate, a coating material and a starch layer; the microporous aggregate includes activated carbon, sponge iron, silicon sulfide and silicon carbide; the coating material includes a carbon source, a nitrogen source, sulfur powder and iron powder; the mass ratio of material A to material B is (1-3):(50-90); preferably, the strain includes Thiobacillus denitrificans, sulfur bacteria and a culture medium, and the contents of Thiobacillus denitrificans and sulfur bacteria in the culture medium are (1-5)×10<supgt;10< / supgt> CFU / ml and (0.5-1.5)×10<supgt;10< / supgt> CFU / ml; the components and contents of the culture medium are as follows: 1-2 g of ammonium chloride, 2-5 g of sodium sulfite, 1-2 g of potassium dihydrogen phosphate, 0.5-1 g of sodium chloride, 0.1-0.2 g of calcium sulfate, and 1 L of water. The catalyst prepared by the present invention is used for sewage treatment, not only has a high denitrification efficiency, but also has a certain reusability, is economical, environmentally friendly and has a low cost.

The present application relates to the technical field of microbial fermentation, and proposes a method for high-density fermentation of Thiobacillus denitrificans by using nitrifying bacteria fermentation waste liquid, which comprises the following steps: taking nitrifying bacteria fermentation waste liquid and initial nutrient solution into a bioreactor, inoculating Thiobacillus denitrificans SZG-SAD-004, and fermenting in an anaerobic environment; the preservation number of the Thiobacillus denitrificans SZG-SAD-004 is CCTCC M 2023992; the nitrifying bacteria fermentation waste liquid is waste liquid produced after co-fermentation of nitrosobacteria, nitrifying bacteria and denitrifying bacteria. When autotrophic nitrifying bacteria is produced by a microbial fermentation method, waste liquid containing high-concentration nitrate, inorganic salt and metal ions is generated, which needs to be treated professionally to reach the discharge standard, thereby increasing the cost of enterprises. The Thiobacillus denitrificans SZG-SAD-004 of the present application can directly utilize the waste liquid for high-density fermentation, without additional treatment, thereby realizing resource utilization of the waste liquid.