1 results about "Thiobacillus denitrificans" patented technology

This invention relates to the field of environmental microbiology, specifically disclosing an autotrophic denitrifying bacterial agent and its preparation method. This autotrophic denitrifying bacterial agent employs a three-layer biomimetic design: a sulfur-calcium composite core, an inner shell gel layer, and an outer functional layer. Denitrifying Thiobacillus is loaded into the core, and Pseudomonas schlegelii is fixed in the outer layer, with a sodium alginate-xanthan gum gel layer providing physical isolation, achieving spatial partitioning and symbiosis of autotrophic and heterotrophic denitrifying bacterial communities. The sulfur-calcium composite core uses sulfur powder as an electron donor, and hydrophobically modified eggshell powder supplements alkalinity in situ. The inner shell layer incorporates ultrafine calcium carbonate, providing both mechanical reinforcement and pH buffering functions. The outer layer encapsulates sodium acetate-stearate microspheres for slow carbon source release. This autotrophic denitrifying bacterial agent exhibits high denitrification efficiency, pH stability, and strong resistance to hydraulic shearing under low carbon-to-nitrogen ratio conditions. The preparation process is mild, and the raw materials are inexpensive and readily available, making it suitable for deep denitrification in wastewater treatment and other scenarios.