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Method for cultivating high density Thiobacillusdenitrificans

A high-density Thiobacillus denitrification technology, applied in the field of bioengineering, can solve the problems of long biological cultivation time, difficult and dangerous operation, single domestication method, etc., and achieve the effect of rich strains, rapid start-up, and diversified domestication methods

Active Publication Date: 2014-05-07
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the deficiencies in the prior art, such as long biological cultivation time, single strain, difficult and dangerous operation, and single domestication method, the object of the present invention is to provide a gas-liquid two-phase culture and reaction method using an air-lift bioreactor. The device startup method has the advantages of rapid cultivation, rich strains and strong pertinence, convenient and safe operation, and diversified domestication methods.

Method used

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  • Method for cultivating high density Thiobacillusdenitrificans
  • Method for cultivating high density Thiobacillusdenitrificans
  • Method for cultivating high density Thiobacillusdenitrificans

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Prepare the required nutrient solution according to the formula, put it into a triangular flask, wrap it, put it in a steam sterilizer, and carry out high-pressure moist heat sterilization, at 121°C~123°C, 0.12 MPa, sterilize for 20~30 min, take it out and cool it to room temperature.

[0029] Using the above sterilized nutrient solution, with S 2 o 3 2- For energy, the temperature is 30 ℃, pH=7, the inoculation amount of Thiobacillus denitrificans is 10%, and the culture is carried out at 0 h, 5 h, 15 h, 20 h, 25 h, 30 h, 40 h, 45 h , 50 h, 55h, 65h, 70 h, 75 h, 90 h, 100 h sampling test OD 420 , with non-inoculated nutrient solution as a control.

[0030] test results figure 2 It shows that Thiobacillus denitrificans grows rapidly, maintains in the lag phase within 15 h, and the number of bacteria increases slightly, and then the bacteria enter the logarithmic growth phase, at this stage (15 h~30 h), the bacteria grow actively and reproduce in large numbers . ...

Embodiment 2

[0032] Using the above sterilized nutrient solution, with S 2 o 3 2- For energy, the inoculum amount of Thiobacillus denitrificans is 10%, pH=7, cultured in the temperature range of 20~40℃, and samples are taken to measure OD 420 , with non-inoculated nutrient solution as a control.

[0033] test results image 3It shows that the optimal growth temperature of Thiobacillus denitrificans is 25~35℃, and 30℃ is the most suitable growth temperature of Thiobacillus denitrificans. The growth of Thiobacillus denitrificans was significantly inhibited when the culture temperature was lower than 25℃; the growth of Thiobacillus denitrificans was significantly inhibited when the culture temperature was higher than 40℃.

Embodiment 3

[0035] Using the above sterilized nutrient solution, with S 2 o 3 2- For energy, the temperature is 30 ℃, the inoculum amount of Thiobacillus denitrificans is 10%, and the cultures are carried out in the range of pH=3~11, and the samples are taken to measure the OD 420 , with non-inoculated nutrient solution as a control.

[0036] test results Figure 4 It shows that when the pH value of Thiobacillus denitrificans is 5~9, especially in the range of 6~8, the OD value of the strain can be maintained at a relatively high concentration, which is because the optimum growth pH range of Thiobacillus denitrificans is 6~8, and partial acidity or partial alkalinity will inhibit the growth of Thiobacillus denitrificans.

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Abstract

The invention belongs to the field of bio-engineering, and relates to a method for cultivating high density Thiobacillus denitrificans (ATCC25259). The method comprises: injecting a bacterial suspension and a nutrition solution to an air-lift bioreactor according to a ratio of 10-100:1; introducing air to replace the traditional mechanical stirring to carry out aeration, and controlling the dissolved oxygen concentration level, wherein the aeration rate is 800-1200 L / h; and maintaining the pH value of the nutrition solution in the air-lift bioreactor at 6-8, maintaining the temperature at 25-35 DEG C, and maintaining the conductivity at 20-60 mS / cm. The bacteria cultivating method has advantages of bacterial cultivating and desulfurization test integration, easy and safe operation, fast bacteria cultivating, sufficient and stable dissolved oxygen supply, rich and strongly-targeting bacteria, diversified acclimation manner and the like.

Description

technical field [0001] The invention belongs to microorganism cultivation technology, is applied to the field of bioengineering, and specifically relates to a method for cultivating high-density Thiobacillus denitrificans. Background technique [0002] The treatment of sulfur-containing industrial waste gas has become an important task in the treatment of industrial "three wastes" and the control of air pollutants. At present, the desulfurization technologies for removing sulfur-containing waste gas at home and abroad can be mainly divided into physical methods, chemical methods and biological methods. Treating sulfur-containing gas with traditional physical or chemical methods has the disadvantages of using a lot of equipment, complicated processes, high energy consumption, and complicated secondary pollution treatment processes. The reason why the microbial method can effectively desulfurize is that the selected microorganisms not only adapt quickly to the exhaust gas, bu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 郝爱香毛松柏孔京朱燕
Owner CHINA PETROLEUM & CHEM CORP
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