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Aquacultural water body cleaning method using microorganism

A technology for microbial purification and aquaculture of water bodies, applied in chemical instruments and methods, biological water/sewage treatment, applications, etc., can solve the problems of reducing pollution components, pollution components, poisonous fish and shrimp, etc., and achieve the effect of reducing toxic effects.

Inactive Publication Date: 2004-11-10
TIANJIN GUARD T & D
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem in the development of aquaculture industry is that part of the bait put in is decomposed by the aerobic microorganisms existing in the water body, and finally produces ammonia or / and ammonium, reduced sulfides, small molecules that are toxic to fish and shrimp. Contaminants such as organic acids
[0004] The various methods mentioned above can only control to a certain extent one or two kinds of pollution components that cause the pollution of aquaculture water bodies.
However, there are often multiple pollution components in the aquaculture water, including both oxidative pollution components and reductive pollution components. The above-mentioned various methods cannot meet the requirements of purifying multiple pollution components at the same time.

Method used

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  • Aquacultural water body cleaning method using microorganism
  • Aquacultural water body cleaning method using microorganism
  • Aquacultural water body cleaning method using microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The cultivation method of photosynthetic bacteria culture body is specifically as follows:

[0015] The first step is to prepare a liquid medium for photosynthetic bacteria:

[0016] Basic medium components for enrichment and isolation of photosynthetic bacteria (g / 1000ml aqueous solution)

[0017] (A)NH 4 Cl 1.0, K 2 HPO 4 0.5, MgCl 2 0.2, NaCl 2.0, CH 3 COONa 0.5, yeast extract 0.1, Na 2 S 0.2, FeCl 3 0.1,

[0018] (B) Filter-sterilized 10% NaHCO 3 aqueous solution,

[0019] mixing (A) and (B) at a ratio of 20:1, adjusting the pH value to 7.2 to 7.5, and preparing a liquid culture medium for photosynthetic bacteria;

[0020] The second step is to cultivate and enrich the culture medium: take 70-80ml of the liquid culture medium of the above-mentioned photosynthetic bacteria, put it into a 100ml large test tube, sterilize it at 121°C for 30 minutes, and then take 10ml of the polluted culture water, Pour it into the large test tube and put it into the anae...

Embodiment 2

[0027] Nitrifying bacteria are divided into two subgroups: nitrosifying bacteria and nitrifying bacteria. Nitrosifying bacteria oxidize ammonia to nitrite nitrogen, and nitrifying bacteria oxidize nitrite nitrogen to nitrate nitrogen.

[0028] The cultivation method of nitrosifying bacteria culture body and nitrifying bacteria culture body is as follows:

[0029] The first step is to prepare liquid culture medium for nitrosifying bacteria and nitrifying bacteria:

[0030] (1) Basic medium components for enrichment and isolation of nitrosative bacteria (g / 1000ml aqueous solution)

[0031] (NH 4 ) 2 SO 4 2.0, NaCl 2.0, MgSO 4 ·7H 2 O 0.5, K 2 HPO 4 1.0, FeSO 4 ·7H 2 O 0.4, CaCO 3 5.0, adjust the pH value of the solution to pH 7.0-7.4, and prepare a liquid medium for nitrosation;

[0032] (2) Basic medium components for enrichment and separation of nitrifying bacteria (g / 1000ml aqueous solution)

[0033] KNO 2 1.3, NaCl 2.0, MgSO 4 ·7H 2 O 0.5, K 2 HPO 4 1....

Embodiment 3

[0037] The culture method of the Thiobacillus denitrificans culture in the denitrifying bacteria is as follows:

[0038] The first step is to prepare a liquid medium for denitrifying bacteria:

[0039] Thiobacillus denitrificans enrichment and isolation basal medium components in denitrifying bacteria (g / 200ml aqueous solution)

[0040] (A) KNO 3 0.4, Na 2 S 2 o 3 ·5H 2 O 1.0, K 2 HPO 4 0.4, NH 4 Cl 0.1, MgSO 4 ·7H 2 O 0.12, NaHCO 3 0.2, FeSO 4 ·7H 2 O 0.002,

[0041] (B) Filter-sterilized 10% NaHCO 3 aqueous solution,

[0042] mixing (A) and (B) at a volume ratio of 100:1, adjusting the pH value to 7.0-7.4, and preparing a liquid culture medium for denitrifying bacteria;

[0043] The second step is to cultivate and enrich the culture medium: under aseptic conditions, insert 70-80ml of the above-mentioned liquid medium into a 100ml sterile large test tube, then insert 20ml of polluted aquaculture water, and put it into an anaerobic workstation for cultivation. ...

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PUM

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Abstract

The invention relates to a process for purifying culturing water mass by utilizing microorganism, wherein microbiological functional bacteria of four various physiological categories, including nitrifying bacteria and nitrosation bacteria, denitrogenation sulfur bacillus in denitrifying bacteria, sulfur bacteria, and photosynthetic bacteria are thrown into the culturing water mass, the throwing amount is 0.05-0.20% of the culturing water mass, while the ratio by volume of various bacteria thrown is, nitrifying bacteria and nitrosation bacteria : denitrogenation sulfur bacillus in denitrifying bacteria : sulfur bacteria : photosynthetic bacteria = (1-4) : (1.6í½3) : (2í½3.6) : (1í½4). The invention can substantially reduce the contaminant in the culturing water mass. íí

Description

technical field [0001] The technical solution claimed in the present invention relates to the biological treatment of water characterized by microorganisms, specifically the method of using microorganisms to purify aquaculture water. Background technique [0002] The increasing development of the aquaculture industry has improved the living standards of the people. However, the problem in the development of aquaculture industry is that part of the bait put in is decomposed by the aerobic microorganisms existing in the water body, and finally produces ammonia or / and ammonium, reduced sulfides, small molecules that are toxic to fish and shrimp. Contaminants such as organic acids. [0003] In recent years, some people have carried out research on the application of microorganisms to the purification of aquaculture water. Through the absorption and utilization of pollutants by microorganisms, the toxicity of pollutants to fish and shrimp has been reduced. From the published pa...

Claims

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Application Information

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IPC IPC(8): A01K61/00C02F3/34C12N1/20
CPCY02A40/81
Inventor 吴新世马建芳刁虎欣梁凤来刘如林王淑芳李明智
Owner TIANJIN GUARD T & D
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