Compound microorganism combination microbial agent, preparation method and application thereof, sludge treatment method for black-odor river
A technology of compound microorganisms and combined inoculants, applied in microorganism-based methods, biochemical equipment and methods, biological water/sewage treatment, etc., can solve the problems of insignificant hydrogen sulfide elimination, low treatment efficiency, and weak degradation ability.
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Embodiment 1
[0062] A composite microbial combination bacterial agent, including bacterial agent A combination, bacterial agent β combination and bacterial agent γ combination;
[0063] The preparation method of described bacterial agent A combination is:
[0064] (1) Inoculate Bacillus coagulans, lactic acid bacteria, saccharomyces, Aspergillus sojae and Thiobacillus denitrifica in liquid medium respectively, and cultivate them at 28~32° C. for 48 hours; the liquid medium contains the following components per liter of medium: 8g of beef extract, 10g of peptone, 3g of yeast extract, 5g of sodium chloride, 1g of potassium dihydrogen phosphate, 0.1g of trace elements, 30g of sucrose, and the rest are distilled water with a pH of 6.8 to 7.2; the trace elements are Mn 2+ , Mg 2+ or Fe 2+ , all used are their sulfates;
[0065] (2) Place the strains cultivated in step (1) in fermenters respectively, mass-produce and cultivate them at 28-32°C for 48 hours, and stir the sterile air for 2 minut...
Embodiment 2
[0097] A composite microbial combination bacterial agent, including bacterial agent A combination, bacterial agent β combination and bacterial agent γ combination;
[0098] The preparation method of described bacterial agent A combination is:
[0099] (1) Inoculate Bacillus coagulans, lactic acid bacteria, saccharomyces, Aspergillus sojae and Thiobacillus denitrifica in liquid medium respectively, and cultivate them at 28~32° C. for 48 hours; the liquid medium contains the following components per liter of medium: 8.8g of beef extract, 11g of peptone, 3.3g of yeast extract, 5.5g of sodium chloride, 1.1g of potassium dihydrogen phosphate, 0.11g of trace elements, 33g of sucrose, and the rest are distilled water with a pH of 6.8 to 7.2; the trace elements are Mn 2+ , Mg 2+ or Fe 2+ , all used are their sulfates;
[0100] (2) Place the strains cultivated in step (1) in fermenters respectively, mass-produce and cultivate them at 28-32°C for 48 hours, and stir the sterile air fo...
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