Composite microbial preparation for culture water body improvement

A technology for microbial preparations and water body improvement, applied in the direction of microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problems of bacterial drug resistance, excessive residual amount of biological drugs in aquaculture, etc., and achieve high live bacteria content and species The effect of less and simple preparation method

Inactive Publication Date: 2014-03-26
陈汇梁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the water used for aquaculture and the environment have been seriously polluted, and the situation of aquaculture drugs has become more and more serious, leading to drug resistance of pathogenic bacteria, and the residual amount of aquaculture biological drugs exceeds the standard, bringing some diseases to consumers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Raw material composition:

[0053] Bacillus subtilis 25wt%

[0054] Bacillus licheniformis 30wt%

[0055] Thiobacillus denitrificans 15wt%

[0056] Carrier and Nutrients 30wt%

[0057] Preparation:

[0058] step 1:

[0059] Respectively inoculate Bacillus subtilis and Bacillus licheniformis in the first culture medium under aseptic conditions, and culture single strain for 48 hours at 30°C-35°C to obtain the seed culture solution of Bacillus subtilis and Bacillus licheniformis, and the live bacteria The number is greater than 5.0×10 9 cfu / ml. Among them, the first medium is composed of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, iron sulfate 30 -35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% (including vitamin H2-2.3wt%, vitamin B12-15wt% aqueous solution) and the rest of water, the pH value is 6.5-6.8 .

[0060] Inoculate Thiobacillus denitrificans in the seco...

Embodiment 2

[0071] Raw material composition:

[0072] Bacillus subtilis 30wt%

[0073] Bacillus licheniformis 20wt%

[0074] Thiobacillus denitrificans 20wt%

[0075] Carrier and Nutrients 30wt%

[0076] Preparation:

[0077] step 1:

[0078] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus licheniformis and the seed culture liquid of Thiobacillus denitrifica were provided.

[0079] Step 2,

[0080] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0081] Step 3,

[0082] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0083] Wherein, based on the total weight of the matrix (carrier and nut...

Embodiment 3

[0087] Raw material composition:

[0088] Bacillus subtilis 25wt%

[0089] Bacillus licheniformis 25wt%

[0090] Thiobacillus denitrificans 15wt%

[0091] Carrier and nutrients 35wt%

[0092] Preparation:

[0093] step 1:

[0094] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus licheniformis and the seed culture liquid of Thiobacillus denitrifica were provided.

[0095] Step 2,

[0096] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0097] Step 3,

[0098]Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0099] Wherein, based on the total weight of the matrix (carrier and nutr...

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PUM

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Abstract

The invention provides a microbial preparation for culture water body improvement, which is prepared from the following raw materials in percentage by weight: 25-30 wt% of Bacillus subtilis, 25-30 wt% of Bacillus licheniformis and 15-20 wt% of Thiobacillus denitrificans. The invention also provides a method for preparing the microbial preparation, which comprises the following steps: sequentially inoculating the Bacillus subtilis, Bacillus licheniformis and Thiobacillus denitrificans seed culture solutions into a culture medium, fermenting to obtain a bacterial solution, and mixing the microbial solution with a carrier and nutrient substances to obtain the microbial preparation. The microbial preparation provided by the invention can obviously improve the quality of the culture water body and enhance the flesh texture of the cultured animals.

Description

technical field [0001] The invention relates to a microbial mixture, in particular to a microbial preparation which can be used for improving fresh water bodies. Background technique [0002] China is the world's largest aquaculture country. According to statistics, China's inland aquaculture water area is 27.34 million hectares, seawater aquaculture area is 4.38 million hectares, and rice field fish farming area is 1.52 million hectares in 2001. Especially in the water towns in southern China, aquaculture plays an important role in agricultural production. Many areas regard aquaculture as an important way for local farmers to get rich. Aquaculture has gradually become one of the pillar industries in aquaculture agriculture. [0003] At the same time, China is also one of the countries with the most serious pollution of aquaculture water. Since ancient times, there has been a saying in China that "raise fish first by raising water". The quality of water is directly related ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/10C12R1/01
Inventor 陈汇梁
Owner 陈汇梁
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