Method for applying phosphate solubilizing bacteria extracted from tea garden soil to leaf lettuce growth promotion
By irrigating the roots of lettuce with phosphate-solubilizing strains extracted from tea garden soil, the problem of insufficient growth promotion of lettuce was solved, and the survival rate of seedlings and the degree of root development were improved, as well as the activity of soil enzymes was enhanced.
Patent Information
- Application Number
- CN202510773109.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-11
- Publication Date
- 2025-09-12
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the prior art, methods for promoting the growth of rapeseed have failed to effectively utilize phosphate-solubilizing bacteria in tea garden soil, resulting in insufficient growth and improvement of soil enzyme activity.
Phosphate-solubilizing strains of Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia or Paenibacillus terrigena extracted from tea garden soil were used to promote the growth of rapeseed lettuce by root irrigation. The specific steps included culturing the strains in LB liquid medium and measuring the OD600 value, and then diluting the volume with physiological saline before root irrigation of the rapeseed lettuce.
It significantly improves the survival rate, root development and biomass of rapeseed seedlings, enhances soil enzyme activity, especially urease, acid phosphatase and peroxidase, and improves the rhizosphere microenvironment.
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Figure CN120615628A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of vegetable planting, and in particular to a method for promoting the growth of rapeseed by applying phosphate-solubilizing bacteria extracted from tea garden soil to rapeseed. Background Art
[0002] Oilseed lettuce is an annual to biennial erect herb. It is smooth and glabrous. The stem is solitary and robust. The leaves at the base are densely packed, sessile, long-lanceolate, acute at the apex, tapering to a semi-amplexicaul base, undulating, serrated, or pinnately divided, and light green. The inflorescence is numerous, with ray-shaped yellow flowers. The achenes are oblanceolate.
[0003] We hope to provide a method for promoting the growth of rapeseed by using phosphate-solubilizing bacteria extracted from tea garden soil. Summary of the Invention
[0004] The present invention aims to provide a method for promoting the growth of rapeseed by using phosphate-solubilizing bacteria extracted from tea garden soil. The specific scheme is as follows: A method for promoting the growth of rapeseed using phosphate-solubilizing bacteria extracted from tea garden soil, comprising the following steps: selecting an activated strain and placing it in a triangular flask containing LB liquid culture medium, culturing it on a reciprocating shaker until the bacterial solution becomes slightly turbid, measuring the absorbance of the bacterial suspension at a wavelength of 600 nm using a spectrophotometer, and determining its OD 600 The value is adjusted to 0.7-0.9, the bacterial solution is aspirated with a pipette, 0.8% physiological saline is added to the fixed volume, and then the lettuce is irrigated with roots; the strain is one of Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia, or Paenibacillusterrigena.
[0005] The temperature of the reciprocating shaker was 30° C., the rotation speed was 200 r / min, and the incubation time was 12 h.
[0006] The results showed that the above five strains have a growth-promoting effect on lettuce. BRIEF DESCRIPTION OF THE DRAWINGS
[0007] Figure 1 Figure 2 is a graph showing the growth-promoting effect of the strains on Brassica rapa (CK is the control group, A1, A2, A3, F2, and F3 correspond to Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia, and Paenibacilluster rigena, respectively, and the above-mentioned example groups are arranged in sequence from left to right in the figure); Figure 2 Figure 2 is a plant root system diagram showing the growth-promoting effect of the strains on Brassica rapa (CK is the control group, A1, A2, A3, F2, and F3 correspond to Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia, and Paenibacilluster rigena, respectively, and the above-mentioned example groups are arranged in sequence from left to right in the figure); Figure 3 is the effect of the strains on the biomass of Brassica rapa (CK is the control group, A1, A2, A3, F2, and F3 correspond to Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia, and Paenibacillusterrigena, respectively); Figure 4 is the effect of the strains on soil enzyme activity (CK is the control group, A1, A2, A3, F2, and F3 correspond to Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia, and Paenibacillusterrigena, respectively). DETAILED DESCRIPTION
[0008] 23.5 cm × 7.3 cm × 14 cm plant observation boxes were used, with 30 lettuce seeds per pot sown. Biological replicates were set up and placed in a constant temperature incubator (daytime: 30°C, 12 h; nighttime: 28°C, 12 h). After one week, seedlings were transplanted to ensure a consistent number of lettuce seeds in each observation box. After one week of incubation, activated strains were selected and placed in 150 mL Erlenmeyer flasks filled with LB liquid medium. The flasks were sealed with sterile sealing film and labeled. Incubation was performed on a reciprocating shaker at 30°C and 200 rpm for 12 h until the suspension became slightly turbid. The absorbance of the suspension was measured at 600 nm using a spectrophotometer. When the OD600 value reached approximately 0.8, indicating a bacterial count of approximately 108 CFU / mL, 5 mL of the suspension was pipetted into a 50 mL centrifuge tube and made up to 50 mL with 0.8% saline for root irrigation. Watering was done once every 5 days, and the cells were allowed to balance for one week after watering for 5 times before measurement.Example CK group, A1 group, A2 group, A3 group, F2 group and F3 group were set up.
[0009] CK is the control group (no strain injection), A1, A2, A3, F2, and F3 correspond to Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia, and Paenibacilluster rigena, respectively (all of the above strains are commercially purchased).
[0010] 1. Phenotypic characteristic parameters of rapeseed The growth characteristics and root development of rapeseed after root irrigation with bacterial solution are shown in Figure 1 、 2 As can be seen from the figure, the phosphate-solubilizing bacteria treatment significantly promoted the growth and development of rapeseed seedlings. The survival rate of rapeseed seedlings after root irrigation with phosphate-solubilizing bacteria was 80.0%, a 6.7% increase compared to the CK (survival rate of 73.3%). In addition, the root system of rapeseed seedlings treated with phosphate-solubilizing bacteria was more developed than that of the CK, with both showing more developed taproots and more fibrous roots than the CK treatment, indicating a more developed root system. The plant height of rapeseed seedlings grew differently under different treatment concentrations, with the plant height showing a trend of F3>CK>A1>A2>A3>F2.
[0011] Plant biomass is an important indicator of plant growth. Morphological characteristics such as plant height and stem diameter directly affect leaf area, photosynthesis and stress resistance, which in turn affect crop growth, development and yield quality. Plant height, stem diameter, root fresh weight, root dry weight, aboveground fresh weight and aboveground dry weight of rapeseed seedlings treated with different bacterial solutions were measured and significant differences were analyzed (see Figure 3 ), the results showed that the fungus treatment increased the fresh weight of the roots and the fresh weight of the aboveground parts of the rapeseed. As shown in the figure, the stem diameter of the A3 treatment was higher than that of the other treatments and significantly higher than that of the F2 ( p <0.05); the root fresh weight of the experimental group was generally higher than that of the CK, and the root dry weight of A3 was higher than that of the other treatment groups and significantly higher than that of F2 ( p <0.05); the plant height of F3 was significantly higher than that of other treatments ( p <0.001), and the plant height of CK treatment was significantly higher than that of A2, A3, and F2 ( p <0.01); the aboveground fresh weights of the experimental groups were higher than those of the CK, and the aboveground dry weights of A3 and F3 were higher than those of the other treatment groups.
[0012] 2 Changes in soil enzyme activity The activities of urease, acid phosphatase, peroxidase and sucrase in the soil after the bacterial treatment were determined. Figure 4As shown in the results, the inoculation treatment had a significant effect on the activity of various enzymes. The urease activity of the experimental group was significantly higher than that of CK (p<0.05). The urease activity of A3 treatment was the highest (320.33U / g), which was 3.04 times that of CK (105.45U / g), indicating that A3 had a stronger nitrogen metabolism. The sucrase activity showed a trend of A3>A1>F3>A2>F2>CK, with the highest value of A3 (38.12mg / g) being 2.26 times that of CK (16.88mg / g). The acid phosphatase activities of A1 and A2 were 15804.82 and 15804.82, respectively. U / g and 13277.88U / g, which were 7.48 and 6.28 times that of CK (2113.43U / g). This may be because the secretion of organic acids by phosphate-solubilizing bacteria promoted the dissolution of insoluble phosphorus, thereby increasing the activity of soil acid phosphatase; the peroxidase content of A3 was 23.61U / g, which was significantly higher than that of CK (6.59U / g), indicating that A3 may improve the soil environment by enhancing the activity of the oxidoreductase system. The above results show that the phosphate-solubilizing bacteria treatment has a significant regulatory effect on the rhizosphere microenvironment of rapeseed.
[0013] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.
Claims
1. A method for promoting the growth of rapeseed using phosphate-solubilizing bacteria extracted from tea garden soil, characterized in that: The activated strain was placed in a triangular flask containing LB liquid culture medium, cultured on a reciprocating shaker until the bacterial suspension was slightly turbid, and the absorbance of the bacterial suspension was measured at a wavelength of 600 nm using a spectrophotometer to determine the OD value. 600 The value is adjusted to 0.7-0.9, the bacterial solution is aspirated with a pipette, 0.8% physiological saline is added to the volume, and then the lettuce is irrigated; the strain is one of Bacillus velezensis, Bacillus thuringiensis, Burkholderia stagnalis, Burkholderia cepacia, or Paenibacillusterrigena.
2. The method for promoting the growth of rapeseed using phosphate-solubilizing bacteria extracted from tea garden soil according to claim 1, wherein: The temperature of the reciprocating shaker was 30° C., the rotation speed was 200 r / min, and the incubation time was 12 h.