Total-flavone extract of lindera root leaves and preparation method and use thereof

A technology of total flavonoids and black leaves, applied in the field of biopharmaceuticals

Inactive Publication Date: 2009-06-10
ZHEJIANG HONGSHILIANG GRP TIANTAISHAN SPICEBUSHROOT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the extraction process and activity of total flavonoids from black leaves

Method used

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  • Total-flavone extract of lindera root leaves and preparation method and use thereof
  • Total-flavone extract of lindera root leaves and preparation method and use thereof
  • Total-flavone extract of lindera root leaves and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Pulverize the medicinal material of black herb leaves into coarse powder, decoct and extract with water 40 times the weight of the material for 3 times, each time for 0.5 hours, filter, cool, and combine the extracts. The extract was passed through a column of LSA-10 macroporous resin (produced by Xi'an Lanshen) and eluted with 60% ethanol, and the eluate was evaporated to dryness after evaporating the solvent. The extract of total flavonoids from Wuyao leaves was obtained, and the content was 91%.

Embodiment 2

[0016] Example 2: Pulverize the medicinal material of black herb leaves into coarse powder, decoct and extract twice with water 25 times the weight of the material, each time for 1 hour, filter, cool, and combine the extracts. The extract was passed through a polyamide column and eluted with 70% ethanol, and the eluent was evaporated to dryness after the solvent was evaporated. The extract of total flavonoids from Wuyao leaves was obtained, and the content was 86%.

[0017] To sum up, the method for extracting total flavonoids in the leaves of black herb leaves of the present invention can effectively extract the total flavonoids from the leaves of black herbs, the total flavonoid content in the extract is higher than 60%, and the process is simple and feasible, and the cost is low. Suitable for industrial production.

[0018] The method for extracting the total flavonoids in the leaves of the black medicine disclosed by the present invention has been described above, but it ...

Embodiment 3

[0019] Embodiment 3: The total flavonoids extract of black leaves leaves obtained by the extraction method of the above-mentioned embodiment 1 is used for pharmacological activity test, pharmacological results and analysis, to prove that the total flavonoids extracts of black leaves are used in the preparation of health food or health care medicine, and the preparation of It is used in the treatment of various acute and chronic viral hepatitis, chemical hepatitis, drug-induced hepatitis and autoimmune hepatitis.

[0020] 1. Pharmacological test of the total flavonoids extract of black medicine leaves

[0021] (1) Determination of total antioxidant capacity and anti-superoxide anion free radical activity unit in chemical simulation system

[0022] With 75% ethanol as the negative control, quercetin (quercetin) as the positive drug, respectively dissolve quercetin and total flavonoids in 75% ethanol to make a sample solution with a certain concentration gradient, respectively:...

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Abstract

The invention relates to a lindera aggregate leaf total flavonoids extract preparing method and application thereof belonging to the biopharmaceutical technology field. The lindera aggregate leaf total flavonoids extracting method comprises the steps: smashing the medicinal materials, boiling by water and extracting, filtering out the sediments and taking the liquid, passing the extract liquid through macroporous resin or polyamide column, eluting by ethanol water solution, concentrating the eluent, and getting extract containing the total flavonoids. The method achieves the advantages of simple, low cost, high total flavonoids content in extract, and suitable for industrial production. The lindera aggregate leaf total flavonoids exact achieves remarkable oxidation resistance and liver protection activity in vivo or in vitro experiments, can be applied in preparation of health food, health medicine and medicines for treating acute / chronic viral hepatitis, chemical hepatitis, drug hepatitis and autoimmunity hepatitis.

Description

1. Technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a method for extracting total flavonoids from leaves of black medicinal material and its application. 2. Background technology [0002] Lindera aggregate (Sims) Kosterm. is a plant of the genus Lindera in the Lauraceae family. It is distributed in Jiangsu, Zhejiang, Taiwan, Fujian, Guangdong and other provinces. It is more famous in Tiantai County, Zhejiang Province, and is called Tiantai Wuyao. Its dried roots are used as medicine, warm in nature and pungent in taste, and have the effects of smoothing Qi, relieving pain, warming kidney and dispelling cold. However, the clinical application and pharmaceutical research of its leaves are less. It is recorded in the literature that the leaves of black medicine can be used to treat acute cellulitis, mastitis and gluteus carbuncle, and it can treat chronic gastritis when it is compatible with Yiweizhitong for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/54A61P1/16A61P31/12A61K127/00
Inventor 陈婷徐强肖梅顾莉蕴张晓陈方标何国庆王利
Owner ZHEJIANG HONGSHILIANG GRP TIANTAISHAN SPICEBUSHROOT
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