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Immunity enhancing agent, inactivated vaccine, and preparation method thereof

A technology of immune enhancer and inactivated vaccine, which is applied in the field of biopharmaceuticals, can solve the problems of external stimuli and side effects in pigs, and achieve the effect of enhancing immune effect, low cost, and improving immune level

Inactive Publication Date: 2013-05-08
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing porcine circovirus inactivated vaccine has relatively large side effects, and needs to be immunized 3 weeks after immunization, which brings serious external stimulation to the pig body

Method used

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  • Immunity enhancing agent, inactivated vaccine, and preparation method thereof
  • Immunity enhancing agent, inactivated vaccine, and preparation method thereof
  • Immunity enhancing agent, inactivated vaccine, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The inactivated porcine O-type foot-and-mouth disease virus liquid (the virus strain is porcine O-type foot-and-mouth disease virus Myanmar 98 strain) was inactivated by diethyleneimine, and the 146s content was 4ug / mL.

[0038] Healthy susceptible piglets aged 6-7 weeks, with liquid-phase blocking ELISA antibody titer ≤ 1:8.

[0039] 2. Vaccine preparation:

[0040] Immunopotentiator 1 contained 2 mg / mL of MPL, 20 mg / mL of MDP and 0.4 mg / mL of β-glucan, and the solvent was distilled water.

[0041] Mix the immune enhancer 1 with the inactivated porcine O-type foot-and-mouth disease virus solution at a volume ratio of 1:3 to prepare an aqueous solution. First place the ISA206 and the aqueous solution at room temperature for about 30 minutes. Put ISA206 into an emulsification tank, put the aqueous phase solution into the emulsification tank at 200 rpm, and stir evenly; stir at 2000 rpm for 10 minutes to obtain Vaccine 1. The volume ratio of aqueous phase solution to I...

Embodiment 2

[0059] The inactivated O-type porcine foot-and-mouth disease virus liquid (the virus strain is O-type porcine foot-and-mouth disease virus Myanmar 98 strain) was inactivated by diethyleneimine, and the 146s content was 5.87ug / mL.

[0060] Healthy susceptible piglets aged 6-7 weeks, with liquid-phase blocking ELISA antibody titer ≤ 1:8.

[0061] 2. Vaccine preparation:

[0062] Prepare immune booster 2. Immunopotentiator 2 contained 2 mg / mL of MPL, 40 mg / mL of MDP and 0.4 mg / mL of β-glucan, and the solvent was distilled water.

[0063] Mix the immunopotentiator 2 with the inactivated porcine foot-and-mouth disease virus solution at a volume ratio of 1:1 to obtain an aqueous phase solution. First place the ISA206 and the aqueous solution at room temperature for about 30 minutes. Put ISA206 into the emulsification tank, put the aqueous phase solution into the emulsification tank at 200 rpm, stir evenly, and stir at 2000 rpm for 10 minutes to obtain vaccine 2. The volume ratio...

Embodiment 3

[0080] The inactivated porcine O-type foot-and-mouth disease virus liquid (the virus strain is porcine O-type foot-and-mouth disease virus Myanmar 98 strain) was inactivated by diethyleneimine, and the 146s content was 5.87ug / mL.

[0081] Healthy susceptible piglets aged 6-7 weeks were tested with a liquid-phase blocking ELISA kit (Lanzhou Veterinary Research Institute) to obtain a liquid-phase blocking ELISA antibody titer ≤ 1:8.

[0082] 2. Vaccine preparation:

[0083] Immunopotentiator 3 contained 0.1 mg / mL of MPL, 2 mg / mL of MDP and 0.1 mg / mL of β-glucan, and the solvent was distilled water.

[0084] Mix the immunopotentiator 3 with the inactivated porcine foot-and-mouth disease virus solution at a volume ratio of 1:1 to obtain an aqueous phase solution. First place the ISA206 and the aqueous solution at room temperature for about 30 minutes. Put ISA206 into the emulsification tank, put the aqueous phase solution into the emulsification tank at 200 rpm, stir evenly, and...

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Abstract

The invention provides an immunity enhancing agent, an inactivated vaccine, and a preparation method thereof. The invention relates to the field of biopharmaceutical. The immunity enhancing agent comprises 0.1-21mg / mL of monophosphoryl lipid A, 1.5-125mg / mL of muramyl dipeptide, and 0.7-4.5mg / mL of beta-glucan. The invention also provides the inactivated vaccine comprising the immunity enhancing agent, and a preparation method of the inactivated vaccine. According to the invention, the immunity enhancing agent is mixed with an inactivated antigen solution, such that a water-phase solution is obtained; and the water-phase solution is mixed with an oil-phase solution, such that the inactivated vaccine is obtained. According to the immunity enhancing agent provided by the invention, with a synergetic effect of the components, body immunity level can be improved, and immune response to antigen can be improved, such that antibody level after immunization can be increased, immune window period can be shortened, and vaccine immunization effect can be enhanced. According to the inactivated vaccine comprising the immunity enhancing agent, antibody level after immunization is high, a protection period is long, and immunization window period is short.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to an immune enhancer, an inactivated vaccine and a preparation method thereof. Background technique [0002] Foot and mouth disease (Foot and mouse disease, FMD) is caused by foot and mouth disease virus (Foot-and-Mouth Disease Virus, FMDV), an acute, hot and highly contact infectious disease that occurs in artiodactyls. At present, this disease is prevalent in many countries in the world, causing great economic losses to animal husbandry. Moreover, the recessive infection of the disease can reduce the production capacity of sick animals and cause huge economic losses. It is listed as the first type of animal disease in my country. [0003] At present, the international community mainly adopts two measures for the prevention and control of foot-and-mouth disease: emergency culling and immunization. In our country, compulsory immunization is mainly adopted, while the policy of bl...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K9/107A61K39/135A61K39/12A61K39/215A61P31/20A61P31/14
Inventor 陈瑾郑其升侯立婷李鹏成于晓明徐海侯继波
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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