Duchesnea polysaccharide, as well as preparation and use thereof

A technology of polysaccharide and snakeberry, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of low bioavailability, high toxicity, low activity, drug resistance, etc., and achieve simple cultivation techniques , easy to reproduce and easy to operate

Inactive Publication Date: 2009-07-22
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above three drugs were used in the treatment of herpes virus in the early stage. Due to the limitation of high toxicity and low activity, they have been replaced by Aci...

Method used

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  • Duchesnea polysaccharide, as well as preparation and use thereof
  • Duchesnea polysaccharide, as well as preparation and use thereof
  • Duchesnea polysaccharide, as well as preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation and identification of snakeberry polysaccharide DIP:

[0049] Swell the dried and pulverized whole snakeberry with water overnight, bathe in water at 100°C for 10 hours, stir once every 1 hour, recover the extract, centrifuge at 6000 rpm for 10 minutes to remove the precipitate, concentrate under reduced pressure, and precipitate with 75% alcohol concentration to obtain DIP. Cytopathic inhibition experiments (please refer to Example 8 for detailed methods) prove that DIP is an antiviral active substance. Using α-naphthol-sulfuric acid color reaction, the obtained solution was identified as orange, which was a characteristic reaction of polysaccharides, proving that the main components obtained were polysaccharides.

Embodiment 2

[0051] Preparation of neutral polysaccharide DIP in snakeberry polysaccharide 1 :

[0052] Make a 1% solution of snakeberry polysaccharide DIP with water, centrifuge to remove impurities, extract according to DIP solution: chloroform: n-butanol (V / V) 25:5:1, shake for 10 minutes, let stand to separate layers, and keep the water phase Layer, repeat the operation 5 times. The upper aqueous solution was concentrated under reduced pressure and precipitated with alcohol. Soak and swell with DEAE-52 ion-exchange column with 10 times of water overnight, change water and rinse 1-2 times, then activate with 0.5mol / L HCl, 0.5mol / L NaOH, 0.5mol / L HCl, vacuum degas, pack column balance. Weigh the deproteinized DIP, make a 0.5% solution with water, centrifuge to remove impurities, and load the sample with a constant flow pump at a flow rate of 1ml / min. After loading the sample, elute with water until there is no positive reaction in the phenol-sulfuric acid method, collect the water el...

Embodiment 3

[0054] Preparation of acidic polysaccharide DIP in snakeberry polysaccharide 2 :

[0055] Make a 1% solution of snakeberry polysaccharide DIP with water, centrifuge to remove impurities, extract according to DIP solution: chloroform: n-butanol (V / V) 25:5:1, shake for 10 minutes, let stand to separate, and keep the water phase Layer, repeat the operation 5 times. The upper aqueous solution was concentrated under reduced pressure and precipitated with alcohol. Soak and swell with DEAE-52 ion-exchange column with 10 times of water overnight, change water and rinse 1-2 times, then activate with 0.5mol / L HCl, 0.5mol / L NaOH, 0.5mol / L HCl, vacuum degassing, pack column balance. Weigh an appropriate amount of DIP after deproteinization, make a 0.5% solution with water, centrifuge to remove impurities, and load the sample with a constant flow pump at a flow rate of 1ml / min. After loading the sample, elute with water until there is no positive reaction in the phenol-sulfuric acid me...

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Abstract

The invention relates to a preparation method of polysaccharide which is extracted from plants and usage thereof, belonging to the field of biopharmaceuticals. The preparation of polysaccharide comprises extracting total polysaccharide DIP which is extracted and separated from Duchesnea indica and has clear varicella-zoster virus activity resistance property, neutral polysaccharide neutral polysaccharide DIP1 and acidic polysaccharide DIP2 in total polysaccharide, and two major active monomer compositions polysaccharide DIP30 and polysaccharide DIP 60 in acidic polysaccharide DIP2. The raw material of Duchesnea indica has wide source range, easy regeneration, easily-operated preparation method, and high reproducibility. The polysaccharide shows significant inhibition on the varicella-zoster virus, is safe, non-toxic and stable, and is a high-quality anti-viral drug candidate.

Description

technical field [0001] The invention relates to a polysaccharide extracted from plants, a preparation method and application of the polysaccharide, and belongs to the field of biopharmaceuticals. Background technique [0002] Varicella-Zoster Virus (VZV) belongs to the family Herpesviridae, subfamily Alphaherpesvirinae, and genus Varicellavirus. 1, HSV-1) and herpes simplex virus type 2 (Herpes Simplex Virus 2, HSV-2) are very similar. Varicella-zoster virus (VZV) is a DNA double-stranded virus with a length of about 125,000 base pairs, which can cause two diseases: chickenpox and herpes zoster. The primary infection is chickenpox, and the reactivation of latent causes herpes zoster. Chickenpox is mostly seen in children aged 1-10, with an incubation period of 2-3 weeks, and the onset is rapid, with prodromal symptoms such as fever, headache, and general burnout. Varicella lesions are mainly in the epidermal acanthocytes, and in individual cases, the lesions can involve t...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P31/22
Inventor 陈建华王婧周安
Owner CHINA PHARM UNIV
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