Duchesnea polysaccharide, as well as preparation and use thereof
A technology of polysaccharide and snakeberry, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of low bioavailability, high toxicity, low activity, drug resistance, etc., and achieve simple cultivation techniques , easy to reproduce and easy to operate
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Embodiment 1
[0048] Preparation and identification of snakeberry polysaccharide DIP:
[0049] Swell the dried and pulverized whole snakeberry with water overnight, bathe in water at 100°C for 10 hours, stir once every 1 hour, recover the extract, centrifuge at 6000 rpm for 10 minutes to remove the precipitate, concentrate under reduced pressure, and precipitate with 75% alcohol concentration to obtain DIP. Cytopathic inhibition experiments (please refer to Example 8 for detailed methods) prove that DIP is an antiviral active substance. Using α-naphthol-sulfuric acid color reaction, the obtained solution was identified as orange, which was a characteristic reaction of polysaccharides, proving that the main components obtained were polysaccharides.
Embodiment 2
[0051] Preparation of neutral polysaccharide DIP in snakeberry polysaccharide 1 :
[0052] Make a 1% solution of snakeberry polysaccharide DIP with water, centrifuge to remove impurities, extract according to DIP solution: chloroform: n-butanol (V / V) 25:5:1, shake for 10 minutes, let stand to separate layers, and keep the water phase Layer, repeat the operation 5 times. The upper aqueous solution was concentrated under reduced pressure and precipitated with alcohol. Soak and swell with DEAE-52 ion-exchange column with 10 times of water overnight, change water and rinse 1-2 times, then activate with 0.5mol / L HCl, 0.5mol / L NaOH, 0.5mol / L HCl, vacuum degas, pack column balance. Weigh the deproteinized DIP, make a 0.5% solution with water, centrifuge to remove impurities, and load the sample with a constant flow pump at a flow rate of 1ml / min. After loading the sample, elute with water until there is no positive reaction in the phenol-sulfuric acid method, collect the water el...
Embodiment 3
[0054] Preparation of acidic polysaccharide DIP in snakeberry polysaccharide 2 :
[0055] Make a 1% solution of snakeberry polysaccharide DIP with water, centrifuge to remove impurities, extract according to DIP solution: chloroform: n-butanol (V / V) 25:5:1, shake for 10 minutes, let stand to separate, and keep the water phase Layer, repeat the operation 5 times. The upper aqueous solution was concentrated under reduced pressure and precipitated with alcohol. Soak and swell with DEAE-52 ion-exchange column with 10 times of water overnight, change water and rinse 1-2 times, then activate with 0.5mol / L HCl, 0.5mol / L NaOH, 0.5mol / L HCl, vacuum degassing, pack column balance. Weigh an appropriate amount of DIP after deproteinization, make a 0.5% solution with water, centrifuge to remove impurities, and load the sample with a constant flow pump at a flow rate of 1ml / min. After loading the sample, elute with water until there is no positive reaction in the phenol-sulfuric acid me...
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