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Method for efficiently separating zhongshengmycin from fermentation liquor

A technology for biotin and fermentation broth, applied in the field of high-efficiency separation of biotin, can solve the problems of many solid hazardous wastes, unfavorable environmental control, complicated preparation process, etc., to reduce production difficulty and quality control cost, and improve sustainable The effect of high development and quality stability

Pending Publication Date: 2022-01-07
陕西麦可罗生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of this process is relatively complicated, and the equipment is relatively backward. The quality index control of production intermediate materials is not considered. It is difficult to popularize the test data. At the same time, it produces a lot of solid hazardous waste, which is not conducive to environmental control.
[0006] The above-mentioned various technical schemes, the quality stability of the preparation process is not high, does not meet the requirements of product purity and high-quality preparation production
Therefore, there is a lack of a more optimized process in this field to meet the market's demand for different original drug products, especially the customization of high-quality, high-stability original drug products

Method used

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  • Method for efficiently separating zhongshengmycin from fermentation liquor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) The fermented liquid is acidified with oxalic acid to pH 2.5-3.0, the ceramic clear liquid before and after adding water is filtered by the ceramic membrane, and collected into high-unit clear liquid tanks and low-unit clear liquid tanks respectively. The potency of bacterial residue is 500-800u / ml, The potency of materials is controlled in the range of 1500-3500u / ml and 5500-8500u / ml, the volume of ceramic clear liquid is 2.3-2.5BV, the yield of materials is controlled in the range of 35-40%, 55-60%, and the total yield is 94.5% above.

[0037] 2) The ion exchange resin model is LX-731-X1 modified resin, the production expansion coefficient is 1.5-2.0, and the packing volume is about 50%. Low unit tank materials enter the separation resin for adsorption, the adsorption feed volume rate is 0.2-1.0BV / h (effective rate 2-4kg / t.h -1 ), the pH of the adsorption end is 6.5-7.0, the total adsorption volume is 70-80BV, and the adsorption capacity is controlled at 130-140kg...

Embodiment 2

[0043] 1) to 3) steps are the same;

[0044] 4) Concentrated mother liquor of eluent and concentrated mother liquor of high unit supernatant are mixed according to (1:1-3:1), spray-dried, and the content of the original drug is 35-45%;

[0045] 5) The concentration of nanofiltration is more than 96%, the yield of spray drying is more than 86%, and the total yield of production is between 78-85%.

Embodiment 3

[0047] 1) to 3) steps are the same;

[0048] 4) After the high unit tank material and eluent are mixed, nanofiltration is concentrated to 40,000-55,000 units, spray-dried, and the content of the original drug is 45-55%;

[0049] 5) The concentration of nanofiltration is more than 96%, the yield of spray drying is more than 86%, and the total yield of production is between 78-85%.

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Abstract

The invention relates to a method for efficiently separating zhongshengmycin from fermentation liquor. The core process method comprises the following steps of: filtering the fermentation liquor by a ceramic membrane, and performing cation resin exchange adsorption separation on low-unit ceramic clear liquid of the ceramic clear liquid, wherein the elution yield is 90-95%; independently concentrating and spray-drying the analytical liquid, or mixing with high-unit concentrated mother liquor of the ceramic clear liquid according to a ratio of 1: 1-1: 3, concentrating and spray-drying, wherein the content of the obtained zhongshengmycin raw drug is 25-65%, the total yield is improved by 16-20% compared with the raw drug, and the color and purity of the raw drug are greatly improved compared with 12-24% granular products in the market. According to the method disclosed by the invention, the problems of low separation efficiency of the low-unit mother liquor of the zhongshengmycin and high degradation rate in the production process are solved, the raw drugs with different mass ratios are obtained, the production yield is improved, and the production cost is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of extraction of agricultural antibiotics, and in particular relates to a method for efficiently separating Zhongshengmycin in fermentation broth. Background technique [0002] Zhongshengmycin is a broad-spectrum antibiotic that can resist Gram-positive bacteria, Gram-negative bacteria, mycobacteria, yeast and filamentous fungi, and is moderately toxic to humans and animals. It is mainly used to prevent and control apple ring spot, cucumber bacterial angular spot, tomato bacterial wilt and other diseases. As a green, low toxicity and low residue agricultural antibiotic fungicide, it has a good market prospect. The chemical name of Zhongshengmycin is streptothricins (streptothricins), and its chemical structure is composed of three parts: streptothricin, gulosamine and lysine side chain, according to the number of side chain lysine from 1 to 7 They are named streptothricin F, E, D, C, B, A and X respectively...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/02C07H1/06
CPCC07H17/02C07H1/06
Inventor 马文艳王卫富闫军军张心心郑鹏飞邓钊周晶晶李晓艳潘忠成李蒲民
Owner 陕西麦可罗生物科技有限公司
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