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Preparation of collagen base biological medical material

A biomedical material, collagen technology, applied in the field of preparation of biomedical materials, can solve the problems of non-scale production, poor stability, limited use, etc., and achieve the effects of strong degradation resistance, high thermal stability, and good market prospects

Inactive Publication Date: 2008-09-17
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is to use natural biomaterials, such as collagen, chitosan, sodium diatomate and other modified biomaterials as wound dressings, hemostatic sponges (fibers), etc. Although these materials have clinical applications, they are due to the shortage of raw materials. , the extraction rate of collagen is low, the mechanical properties of the material are poor, the stability is poor, and the degradation speed is too fast, etc., the application in trauma, burn treatment, plastic surgery and tissue engineering research is limited, and there is no large-scale production
The collagen-based biomedical materials reported in the prior art or research are almost all type I collagen or collagen hydrolyzate extracted from animal tissues by enzymatic or acid methods, which makes the mechanical properties, thermal stability, and resistance of collagen materials Lower degradation performance, which further limits its use
The studies mentioned above, although many improvements have been made, still cannot meet the needs of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Pretreatment of raw materials

[0034] Take 100 parts of fresh animal skin and wash it with deionized water to remove surface fat, muscle and oil film, add 100 parts of deionized water, heat up to 30°C, add 1.8 parts of soda ash, 1.5 parts of edible oil cleaning agent, AS1.398 protease 0.15 parts, keep warm and stir for 120 minutes, remove hair, skin and grease, and then wash with deionized water for later use.

[0035] Take 100 parts of fresh animal tendons and wash them to remove surface fat, muscle and fascia, add 100 parts of deionized water, heat up to 15°C, add 1.0 parts of soda ash, 0.5 parts of edible oil cleaning agent, keep warm and stir for 120 minutes to remove grease , and then rinsed with deionized water for later use.

[0036] (2) Preparation of natural collagen fiber gel

[0037] Take 100 parts of treated animal skin or tendon, add 300 parts of deionized water, add acid to adjust the pH value to 2.5, treat at 35°C for 10 hours, and then mince or ma...

Embodiment 2

[0043] (1) Pretreatment of raw materials

[0044] Take 100 parts of fresh animal skin and wash it with deionized water to remove surface fat, muscle and oil film, add 200 parts of deionized water, heat up to 40 ° C, add 0.3 parts of soda ash, 2.0 parts of edible oil cleaning agent, AS1.398 protease 0.4 parts, keep warm and stir for 60 minutes, remove hair, skin and grease, and then wash with deionized water for later use.

[0045] Take 100 parts of fresh animal tendon and wash to remove surface fat, muscle and fascia, add 200 parts of deionized water, heat up to 35°C, add 0.3 part of soda ash, 1.0 part of edible oil cleaning agent, keep warm and stir for 60 minutes, remove Grease, and then rinse with deionized water for later use.

[0046] (2) Preparation of natural collagen fiber gel

[0047] Take 100 parts of pretreated animal skin or tendon, add 1000 parts of deionized water, adjust the pH value to 1.5 with acid, treat it at a temperature of 10°C for 10 hours, then mince ...

Embodiment 3

[0053] (1) Pretreatment of raw materials

[0054] Take 100 parts of fresh animal skin and wash it with deionized water to remove surface fat, muscle and oil film, add 1 to 2 times of deionized water, raise the temperature to 35°C, add 1.0 parts of soda ash, 1.5 parts of edible oil cleaning agent, AS1. 0.3 parts of 398 protease, keep warm and stir for 90 minutes, remove hair, skin and grease, and then wash with deionized water for later use.

[0055] Take 100 parts of fresh animal tendons and wash them to remove surface fat, muscle and fascia, add 1 to 2 times deionized water, heat up to 25°C, add 0.7 parts of soda ash, 0.8 parts of edible oil cleaning agent, keep warm and stir for 80 minutes, Remove grease, then rinse with deionized water for later use.

[0056] (2) Preparation of natural collagen fiber gel

[0057] Take 100 parts of treated animal skin or tendon, add 7 times of deionized water, add an appropriate amount of acid to control the pH value to 2.0, treat at 20°C ...

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Abstract

The invention discloses a preparation method for collagen-based biomedical materials, which is characterized in that: fresh animal skin and / or tendon and / or sheet-beam are adopt to make collagen fiber dispersion liquid (gel) or freeze-dry the collagen fiber materials via the enzymatic method, then are immersedly treated for 20 to 60 minutes through ethanol alcohol solution with concentration of 35 to 65 percent with 8 to 12g / L 2- sulfonic acid under the temperature of 15 to 40 DEG C, and then are immersedly treated for 4 to 24 hours through solution with 1.0 to 5.5g / L N-hydroxy amber imide and 0.8 to 4.0g / L carbodiimide hydrochloride under the temperature of 15 to 40 DEG C; the collagen raw materials are cleaned and immersed for two times through ethanol with concentration of 80 to 95 percent, each time for 10 to 30 minutes, then are immersed and cleaned for one time through Na2HPO4 solution with concentration of 1.5 to 2.0 percent and NaCl solution with concentration of 5.5 to 6.5 percent respectively, the time is 30 to 60 minutes; then are immersed and cleaned for 2 times through distilled water immersion, each time for 10 to 30 minutes, the materials after being cleaned are freeze-dried, and the collagen-based biomedical materials are obtained.

Description

1. Technical field [0001] The invention relates to a preparation method of collagen-based biomedical materials, belonging to the field of preparation of biomedical materials. 2. Background technology [0002] Medical biomaterials play a very important role in clinical treatment and medical scientific research, especially in trauma, burn treatment, in vivo tissue repair and plastic surgery, tissue engineering research and application, and are also tissue engineering realization industries. key to transformation. At present, there are two main categories of medical biomaterials that have been developed. One is chemical synthesis, mainly polylactic acid (PLA), polyglycolic acid (PGA), PVA, and PVP. These materials have biocompatibility, degradation speed, etc. There are still many problems unresolved, and its fatal flaw is that it will cause aseptic inflammation in the tissue around the material. Bi Hong and others solved this problem by synthesizing a new material, a copolym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L31/04A61L27/24A61L27/56
Inventor 王坤余琚海燕陈晓威苏德强
Owner SICHUAN UNIV
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