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Process for extracting phospholipid rich-containing eicosapentaenoic acid and docosahexenoic acid

A technology of docosahexaenoic acid and eicosapentaenoic acid, which is applied in the direction of edible phospholipid compositions, medical preparations containing active ingredients, and pharmaceutical formulas. Growth and development, high development and utilization value, and the effect of enhancing immunity

Inactive Publication Date: 2008-12-31
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production of phospholipids at home and abroad is mainly extracted from soybeans and egg yolks. Limited by the characteristics of the natural fatty acid composition of raw materials, it is impossible to obtain phospholipids rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).
There is no report on the use of supercritical carbon dioxide extraction to remove neutral lipids and cholesterol in marine animal raw material powder, and then use ethanol aqueous solution to extract phospholipids rich in EPA and DHA.

Method used

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  • Process for extracting phospholipid rich-containing eicosapentaenoic acid and docosahexenoic acid
  • Process for extracting phospholipid rich-containing eicosapentaenoic acid and docosahexenoic acid
  • Process for extracting phospholipid rich-containing eicosapentaenoic acid and docosahexenoic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Get tap water and clean cod roe, remove impurity, after adding the water of 4 times of weight, make homogenate with colloid mill, gained homogenate is through 12h vacuum freeze-drying, after pulverizing with pulverizer, obtain water content and be 4.0% (weight percent , the same below), get 100g of the cod roe powder and put it into the extraction kettle of a supercritical carbon dioxide extraction device, remove neutral fat and cholesterol in the egg powder through supercritical carbon dioxide extraction and secondary separation, and the pressure in the extraction kettle is 30MPa, the temperature is 60°C; the first-stage separation pressure is 8MPa, the separation temperature is 45°C; the second-stage separation pressure is 6MPa, the separation temperature is 35°C, the flow rate of carbon dioxide is 50ml / g, and the extraction time is 6h. Take out the cod roe powder that has removed the neutral fat and cholesterol from the kettle, add 15 times the weight of 90% ethanol a...

Embodiment 2

[0020] Get tap water to clean the squid roe, remove impurities, add 6 times the weight of water, and use a colloid mill to make a homogenate. The resulting homogenate is vacuum freeze-dried for 24 hours and pulverized with a pulverizer to obtain squid roe with a water content of 5.0%. powder, get 200g of it and put it into the extraction kettle of a supercritical carbon dioxide extraction device, remove the neutral fat and cholesterol in the egg powder through supercritical carbon dioxide extraction and secondary separation, the pressure in the extraction kettle is 30MPa, and the temperature is 45°C; The separation pressure of the first stage is 13MPa, the separation temperature is 45°C; the separation pressure of the second stage is 8MPa, the separation temperature is 40°C, the flow rate of carbon dioxide is 100ml / g, the extraction time is 8h, and the neutralization is removed from the extraction kettle The squid roe powder of fat and cholesterol, add the 95% ethanol aqueous s...

Embodiment 3

[0022] Take tap water to wash the sea urchin gonads, remove impurities, add 5 times the weight of water, and use a colloid mill to make a homogenate. The obtained homogenate is vacuum freeze-dried for 16 hours and crushed with a pulverizer to obtain sea urchin gonads with a water content of 4.5%. Take 250g of it and put it into the extraction kettle of the supercritical carbon dioxide extraction device, remove the neutral fat and cholesterol in the sea urchin gonad powder through supercritical carbon dioxide extraction and secondary separation, the pressure in the extraction kettle is 25MPa, and the temperature is 55°C ;The separation pressure of the first stage is 13.5MPa, the separation temperature is 50°C; the separation pressure of the second stage is 9MPa, the separation temperature is 45°C, the flow rate of carbon dioxide is 150ml / g, the extraction time is 10h, take it out from the extraction kettle The sea urchin gonad powder of neutral fat and cholesterol is extracted b...

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Abstract

A method for extracting a phospholipid rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is characterized in that firstly marine animal materials are grinded into homogenate solution with colloid; the homogenate solution is frozen and dried and then crushed through a crushing mill; neutral fat and cholesterol in the material powder are removed through supercritical carbon dioxide; and then the phospholipid is extracted with ethanol aqueous solution. The product extracted through the method has high yield and purity; the extraction process has no pollution to the environment and the production staff; the extracted product can promote growth and development of infant brain, enhance memory, promote infant optic nerve and retina development and prevent and cure heart vascular diseases, and has anti-inflammatory, anti-cancer, immunity-enhancing and other physical activities, thereby having higher development and utilization value as health care products or raw materials of medicine.

Description

technical field [0001] The invention relates to a method for extracting phospholipids rich in eicosapentaenoic acid and docosahexaenoic acid from marine animal raw materials. Background technique [0002] Phospholipids are an important component of cell membranes, and are widely used in medicine, cosmetics, and food industries as liposomes and emulsifiers. The physiological activity of phospholipids is closely related to the composition of its fatty acids and the properties of polar ends, and some phospholipids with specific structures have high medicinal value. Phospholipids are one of the main components of the cell membrane. They are also the storage form of many information molecule precursors on the cell membrane. They play an important role in the regulation of body functions and have a variety of nutritional and health functions: regulating human metabolism, enhancing physical fitness, strengthening the brain, Nourishes the brain, eliminates brain fatigue, and improv...

Claims

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Application Information

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IPC IPC(8): C07F9/10A61K35/56A61K35/60A61K35/616A61K35/618
CPCY02P20/54
Inventor 薛长湖王琦李兆杰薛勇王玉明唐庆娟徐杰赵玉然
Owner OCEAN UNIV OF CHINA
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