Fluorescent DNA-silver nanocluster, and preparation method and application thereof

A silver nanocluster and fluorescence technology, applied in the field of analysis and detection, can solve the problems of inability to use conventional detection applications and high cost

Active Publication Date: 2016-10-12
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF4 Cites 27 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it requires elaborate design, synthesis, and purification by specialized ins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent DNA-silver nanocluster, and preparation method and application thereof
  • Fluorescent DNA-silver nanocluster, and preparation method and application thereof
  • Fluorescent DNA-silver nanocluster, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment one (best synthesis condition):

[0046] A method for preparing fluorescent DNA-silver nanoclusters, which includes the following steps: mix 15uL, 100μM sequence of 5'-ACC CGA ACC TGG GCT ACC CAC CCC TTA ATC CCC-3' oligo-stranded DNA mother solution with 73uL of phosphate buffer Mix, then transfer the resulting mixed solution to a constant temperature metal bath, heat at 90°C for 5 minutes, then take out the mixed solution and slowly cool it down to room temperature, then add 6ul of AgNO with a concentration of 1.5mM to the mixed solution 3 solution (the molar ratio of oligo-stranded DNA to Ag+ is 1:6), shake vigorously for 5 minutes and then let it stand for 30 minutes, then add 6ul of NaBH with a concentration of 1.5mM to the mixed solution 4 Solution (by molar ratio BH 4 - : Ag + =1:1 add NaBH 4 solution), oscillated for 8 minutes to mix, and reacted at room temperature in the dark for 6 hours to obtain the fluorescent DNA-silver nanocluster solution. ...

Embodiment 2

[0049] A method for preparing fluorescent DNA-silver nanoclusters, which comprises the following steps: mix 10uL, 100uM sequence of 5'-ACC CGA ACC TGG GCT ACC CAC CCC TTA ATC CCC-3' oligo-stranded DNA mother solution with 84uL of phosphate buffer Mix, then transfer the resulting mixed solution to a constant temperature metal bath, heat at 80°C for 3 minutes, then take out the mixed solution and slowly cool it down to room temperature, then add 3ul of AgNO with a concentration of 1mM to the mixed solution 3 Solution (oligo-stranded DNA and Ag + The molar ratio is 1:3), shake vigorously for 3 minutes and then let it stand for 20 minutes, then add 3ul of NaBH with a concentration of 2mM to the mixed solution 4 Solution (by molar ratio BH 4 - : Ag + =2:1 add NaBH 4 solution), oscillated for 5 minutes to mix, and reacted at room temperature in the dark for 8 hours to obtain the fluorescent DNA-silver nanocluster solution.

[0050] The pH value of the phosphate buffer solution ...

Embodiment 3

[0052] A method for preparing fluorescent DNA-silver nanoclusters, comprising the following steps: mix 20uL, 100uM sequence of 5'-ACC CGA ACC TGG GCT ACC CAC CCC TTA ATC CCC-3' oligo-stranded DNA mother solution with 60uL of phosphate buffer Mix, then transfer the resulting mixed solution to a constant temperature metal bath, heat at 85°C for 4min, then take out the mixed solution and slowly cool it down to room temperature, then add 8ul of AgNO with a concentration of 3mM to the mixed solution 3 solution (the molar ratio of oligo-stranded DNA to Ag+ is 1:12), shake vigorously for 4 minutes and then let it stand for 25 minutes, then add 12uL of NaBH with a concentration of 3mM to the mixed solution 4 Solution (by molar ratio BH 4 - : Ag + =1.5:1 Add NaBH 4 solution), shake for 6 minutes and mix well, and react at room temperature in the dark for 12 hours to obtain the fluorescent DNA-silver nanocluster solution.

[0053] The pH value of the phosphate buffer solution is 7.2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a fluorescent DNA-silver nanocluster, and a preparation method and application thereof. The particle size of the fluorescent DNA-silver nanocluster is 2-10nm. A water solution of the fluorescent DNA-silver nanocluster looks light pink by visual inspection, and looks shiny red under irradiation of an ultraviolet lamp with the wavelength of 360nm. The water solution is excited by light with the excitation wavelength of 530nm, the emission wavelength is 570-800nm, and high fluorescence emission strength is acquired at the wavelength of 618nm; rhodamine B is taken as a reference substance, and fluorescence quantum yield is 35.3%. A method for detecting cupric ions and pyrophosphate ions by the fluorescent DNA-silver nanocluster includes (1), drawing a response standard curve of the corresponding ions to acquire a linear regression equation; (2), substituting the detected fluorescence strength into the linear regression equation to obtain the concentration of the cupric ions or the pyrophosphate ions. The fluorescent DNA-silver nanocluster has the advantages of stable performance and environment friendliness, the detection method is rapid and simple, and the detection sensitivity is high.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a fluorescent DNA-silver nanocluster and its preparation method and application. Background technique [0002] As an important biological functional anion, pyrophosphate (PPi) plays a very important role in life science, environmental science, pharmaceutical field and chemical process. PPi is an anion with important physiological functions in organisms, and its physiological concentration in healthy individuals is about 3mmol / L. As a product of the hydrolysis of adenosine triphosphate in living cells, PPi participates in many metabolic energy conversion processes in organisms, and plays an important role in physiological processes such as energy storage, signal transduction, DNA replication, and genetic information processing. Some diseases are closely related to PPi content: calcium pyrophosphate dihydrate crystal accumulation is often found in patients with osteoarthritis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 许惠凤叶蕻芝朱希王丽丽余丽双郑春松
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap