Corynebacterium glutamate for synthesizing geraniol and construction method and application of corynebacterium glutamate

A technology of glutamic acid sticks and construction methods, applied in the biological field, to achieve the effect of short fermentation time
CN110438145AInactive Publication Date: 2019-11-12TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2019-11-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a corynebacterium glutamate for synthesizing geraniol and a construction method and application of corynebacterium glutamate. The construction method of corynebacteriumglutamate comprises the following steps: connecting a saccharomyces cerevisiae-derived mutant geranyl pyrophosphate synthase gene ERG20<F96W-N127W> and a valerian-derived geranol synthase gene tVoGEStruncated through cutting off of C-terminal 53 amino acid residues by fusion PCR, adding a ribosome binding site sequence to a homologous region, inserting SacI and XbaI enzyme cutting sites of a corynebacterium glutamate expression plasmid pEC-XK99E to obtain a plasmid 1; converting the plasmid 1 into corynebacterium glutamate to obtain corynebacterium glutamate 1 for synthesizing geraniol. Theconstruction method provides more precursors and short fermentation time (42 to 48 hours) for the synthesis of geraniol, and corresponding by-products are not detected out by GC-MS detection.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a coryneform bacterium glutamicum for synthesizing geraniol, a construction method and application. Background technique

[0002] Geraniol, also known as geraniol, is a colorless or yellow oily liquid at room temperature, with a mild rose scent and a bitter taste. It is often used as a food additive, the main ingredient of rose essence, and an indispensable flavoring raw material in various floral essences. It can also be used as a sweetener, and can also be used to prepare food, soap, and daily cosmetic essences. Products made from natural essential oils contain more than 90% geraniol when preparing food and daily cosmetic flavors. Industrial geraniol is the raw material for the manufacture of vanillyl alcohol, ionone and vitamin A.

[0003] The raw material of the medicine is used for antibacterial and deworming. It is effective in the clinical treatment of chronic bronchitis. It...

Claims

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