Phytoene synthase of endophytic fungi of saffron, encoding gene of phytoene synthase and application of encoding gene

A technology of phytoene and endophytic fungi, which is applied in the field of genetic engineering and can solve problems such as failure to discover the function verification of phytoene synthase

Active Publication Date: 2017-01-04
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology

[0003] At present, whether there is phytoene synthase in saffron and the functional ve

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  • Phytoene synthase of endophytic fungi of saffron, encoding gene of phytoene synthase and application of encoding gene
  • Phytoene synthase of endophytic fungi of saffron, encoding gene of phytoene synthase and application of encoding gene
  • Phytoene synthase of endophytic fungi of saffron, encoding gene of phytoene synthase and application of encoding gene

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[0038] The present invention will be described in detail below with reference to the drawings and specific embodiments.

[0039] The open reading frame sequence of the phytoene synthase gene of the endophytic fungus saffron saffron is as shown in the nucleotide sequence of SEQ ID No.1. The full-length cDNA sequence of the endophytic fungus phytoene synthase gene of Saffron saffron has the nucleotide sequence shown in SEQ ID No.3. The full length of the sequence is 1501 bp, and the sequence includes 176 bp 5'non-coding region and 1218 bp open There is a 46bp intron at 556bp in the reading frame and 61bp 3'non-coding region. This gene encodes 405 amino acids.

[0040] Among them, the biological name of the endophytic fungus of Saffron is Cadophora luteo-olivacea., and its designated name is 3P1CQ1. It is currently stored in the General Microbiology Center of the Chinese Microbial Culture Collection Management Committee, the preservation number is: CGMCCNO.11606, and the preservation...

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Abstract

The invention relates to phytoene synthase of endophytic fungi of saffron, an encoding gene of the phytoene synthase and application of the encoding gene, belongs to the technical field of gene engineering, and further discloses an open reading frame sequence of the gene of the phytoene synthase of the endophytic fungi of the saffron and an amino acid sequence encoded by the gene of the phytoene synthase of the endophytic fungi. The phytoene synthase, the encoding gene and the application have the advantages that typical phytoene synthesis functions of proteins encoded by the gene of the phytoene synthase of the endophytic fungi of the saffron are verified by means of color genetic complementation reaction, accordingly, 2-molecule geranyl geranyl pyrophosphate can be catalyzed to synthesize phytoene, or the phytoene synthase can be used for catalyzing synthesis of terpenoid such as carotenoids.

Description

Technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a saffron endophytic fungus phytoene synthase, its coding gene and application. Background technique [0002] Phytoene synthase ((phytoene shythase, CrtB, PSY) is a key enzyme for the synthesis of terpenoids such as phytoene and other carotenoids. Geranylgeranylpyrophosphate synthase (Geranylgeranylpyrophosphate) synthase, GGPPS) can catalyze the condensation of 15-carbon farnesyl pyrophosphate (FPP) and 5-carbon isopentenyl pyrophosphate (IPP) to produce 20-carbon geranyl geranyl pyrophosphate (GGPP), GGPP is The key precursors of terpenoids and their carotenoids biosynthesis. Geranyl geranyl pyrophosphate (GGPP) can synthesize phytoene under the action of phytoene synthase (CrtB, PSY) For reference figure 1 . [0003] At present, whether there is phytoene synthase in saffron and the functional verification of phytoene synthase in saffron is currently not foun...

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Application Information

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IPC IPC(8): C12N15/54C12N9/10C12N1/14C12P5/02C12P23/00C12R1/645
CPCC12N9/1085C12P5/026C12P23/00C12Y205/01032
Inventor 赵军朱砚姝焉兆萍曾子金
Owner SHANGHAI NORMAL UNIVERSITY
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