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Phytoene synthase from saffron endophytic fungus, its coding gene and application

A technology of phytoene and endophytic fungi, which is applied in the field of genetic engineering and can solve the problem that the function verification of phytoene synthase is not found and the like

Active Publication Date: 2019-12-10
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology

[0003] At present, whether there is phytoene synthase in saffron and the functional verification of phytoene synthase in saffron has not been found in the patent literature

Method used

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  • Phytoene synthase from saffron endophytic fungus, its coding gene and application
  • Phytoene synthase from saffron endophytic fungus, its coding gene and application
  • Phytoene synthase from saffron endophytic fungus, its coding gene and application

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Embodiment Construction

[0038] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0039] The phytoene synthetase gene of the endophytic fungus saffron, the open reading frame sequence of which is the nucleotide sequence shown in SEQ ID No.1. The full-length cDNA sequence of the endophytic fungus phytoene synthase gene of saffron has the nucleotide sequence shown in SEQ ID No.3, the full-length sequence is 1501bp, and the sequence includes a 176bp 5' end non-coding region and a 1218bp open There is a 46bp intron at 556bp in the reading frame and 61bp 3' end non-coding region. This gene encodes 405 amino acids.

[0040] Among them, the biological name of the endophytic fungus of saffron is Cadophora luteo-olivacea., and its designated name is 3P1CQ1. It is currently preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation number is: CGMCCNO.11606, and the pre...

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Abstract

The invention relates to phytoene synthase of endophytic fungi of saffron, an encoding gene of the phytoene synthase and application of the encoding gene, belongs to the technical field of gene engineering, and further discloses an open reading frame sequence of the gene of the phytoene synthase of the endophytic fungi of the saffron and an amino acid sequence encoded by the gene of the phytoene synthase of the endophytic fungi. The phytoene synthase, the encoding gene and the application have the advantages that typical phytoene synthesis functions of proteins encoded by the gene of the phytoene synthase of the endophytic fungi of the saffron are verified by means of color genetic complementation reaction, accordingly, 2-molecule geranyl geranyl pyrophosphate can be catalyzed to synthesize phytoene, or the phytoene synthase can be used for catalyzing synthesis of terpenoid such as carotenoids.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a phytoene synthetase of the saffron endophyte, its coding gene and its application. Background technique [0002] Phytoene synthase (phytoene shythase, CrtB, PSY) is a key enzyme for the synthesis of terpenoids such as phytoene and other carotenoids. Geranylgeranylpyrophosphate synthase synthase (GGPPS) can catalyze the condensation of 15-carbon farnesyl pyrophosphate (FPP) and 5-carbon isopentenyl pyrophosphate (IPP) to generate 20-carbon geranylgeranyl pyrophosphate (GGPP), GGPP is The key precursor for the biosynthesis of terpenoids and their carotenoids. Geranylgeranyl pyrophosphate (GGPP) can synthesize phytoene under the action of phytoene synthase (CrtB, PSY) , can refer to figure 1 . [0003] Whether there is phytoene synthase in saffron and the functional verification of phytoene synthase in saffron has not been found in the current patent lit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/10C12N1/14C12P5/02C12P23/00C12R1/645
CPCC12N9/1085C12P5/026C12P23/00C12Y205/01032
Inventor 赵军朱砚姝焉兆萍曾子金
Owner SHANGHAI NORMAL UNIVERSITY
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