Fusion gene expressing keto carotenoid, recombinant vector and application thereof

Abstract

The invention provides a fusion gene expressing ketone carotenoid, a recombinant vector and application thereof, and relates to the technical field of transgene. In the present invention, phytoene synthase gene CrtB, β-carotene hydroxylase gene HpBHY, β-carotene ketolase gene CrBKT and hemoglobin gene VHB are constructed into a fusion gene, and 2A connections are used to make the genes express efficiently and consistently , under the regulation of the CaMV35S promoter, through transformation and regeneration, the leaves, stems, flowers, seeds of the transgenic plants, and the oil after seed pressing all showed obvious red; and the total carotenoid content in the seeds of the transgenic plants was increased, Among them, the content of ketone carotenoid and astaxanthin is significantly increased; the content of linoleic acid in the transgenic rapeseed is increased; the antioxidant capacity of the transgenic rapeseed is improved.

Description

technical field [0001] The invention belongs to the field of transgenic technology, and specifically relates to a fusion gene for expressing ketone carotenoids, a recombinant vector and applications thereof. Background technique [0002] Carotenoids are the second largest pigment except chlorophyll, and have important functions in light and metabolism, photoprotection, and anti-oxidation of plants and autotrophic microorganisms. Ketocarotenoids such as canthaxanthin and astaxanthin have a People's health plays an important role. Taking astaxanthin as an example, its antioxidant capacity is more than 10 times that of lutein, zeaxanthin, and β-carotene, and 100 times that of vitamin E. Experiments have proved that astaxanthin can effectively improve human immunity, and has obvious effects in preventing cardiovascular diseases, diabetes, cancer and other diseases; and because of its good coloring ability, astaxanthin is widely used in aquaculture, medicine, cosmetics and healt...

AI Technical Summary

Problems solved by technology

By comparison, the content of natural astaxanthin in Haematococcus pluvialis is relatively high, which can reach 4% of dry cell weight, and is the main source of natural astaxanthin production, but Haematococcus pluvialis grows slowly and has an autotrophic cycle. Long, easy to pollute, and difficult to extract astaxanthin from algae walls have become the bottleneck of mass production of astaxanthin

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