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Bacillus subtilis and application thereof

A technology of bacillus subtilis and bacterial strains, applied in the field of bacillus subtilis and its application, can solve the problems of little research and application, and achieve the effects of easy industrial production, prevention and treatment of tree peony brown spot, and simple cultivation conditions

Active Publication Date: 2014-12-10
益地国际农业(北京)有限公司
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  • Abstract
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Problems solved by technology

Biological control is a prevention and control method that uses organisms or their products to control the occurrence of agricultural and forestry diseases and insect pests. Because it is safe for humans and animals, has no pollution to the environment, is not easy to produce drug resistance, has high efficiency and broad spectrum, and can promote crop growth, it is increasingly favored by people. However, there are few researches and applications of biological control on peony disease defense

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

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Embodiment Construction

[0018] The present invention has screened and isolated Bacillus subtilis ( Bacillus subtilis ) HS5B5, the strain is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC No. 6088. The bacterial strain has a strong inhibitory effect on the pathogenic bacteria Cladosporium paeoniae of tree peony brown spot, and can be used for preparing biological preparations for preventing and treating tree peony brown spot.

[0019] 1. Screening and isolation method of strains

[0020] 1. Isolation of Bacillus in samples

[0021] (1) In the peony cultivation area of ​​Luoyang International Peony Garden, the surface soil of peony roots was collected, and 100 samples were collected at different locations, bagged and brought back to the laboratory for air-drying. After the air-dried samples are ground, weigh 10g from each sample and add them to a triangular flask filled with 90mL sterile water ...

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Abstract

The invention belongs to the field of biotechnology and particularly relates to Bacillus subtilis and application thereof. The Bacillus subtilis is Bacillus subtilis HS5B5. The strain is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6088. The Bacillus subtilis HS5B5 provided by the invention has a very strong restraining effect on the pathogen Cladosporiwm paeoniae of brown spot of peony, and can effectively prevent and cure brown spot of peony. The Bacillus subtilis HS5B5 is harmless to the ecological environment and cannot cause the resistance of the pathogen. The Bacillus subtilis HS5B5 is cultured under simple conditions, is easy to preserve, facilitates industrial production and has a favorable development and application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacillus subtilis and an application thereof. Background technique [0002] There are few pests on peony, but there are many types of diseases and serious damage. The disease is that peony is infected by pathogenic microorganisms or affected by adverse environmental conditions during its growth and overwintering process, causing a series of pathological changes from physiological mechanism to morphological structure. Among the more than 20 kinds of peony diseases that have been identified, most of them are fungal diseases, such as brown spot, gray mold, rust, white silkworm and purple feather disease. They cause partial necrosis of leaves or the death of whole leaves, harming the leaves, branches and rhizomes of peony, causing the peony to fall leaves in advance and affecting the vitality. Peony brown spot is one of the most serious diseases. This disease mainly damag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00C12R1/125
Inventor 宋鹏刘领马占强赵杏利刘晓亮刘晨洲杨敏
Owner 益地国际农业(北京)有限公司
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