Bacillus subtilis Y13 for effectively preventing and treating camellia anthracnose and use thereof

A technology for Bacillus subtilis, Camellia oleifera anthracnose, applied in the application, bacteria, fungicides and other directions, can solve the problems of weak or even blank technology, no biological pesticides for preventing Camellia oleifera anthracnose, etc., achieves good development and application prospects, easy industrial production, The effect of simple culture conditions

Inactive Publication Date: 2009-07-08
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Separation and screening of Camellia oleifera anthracnose antagonism biocontrol strains obtained from Camellia oleifera plants is of great significance to the prevention and treatment o

Method used

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  • Bacillus subtilis Y13 for effectively preventing and treating camellia anthracnose and use thereof
  • Bacillus subtilis Y13 for effectively preventing and treating camellia anthracnose and use thereof
  • Bacillus subtilis Y13 for effectively preventing and treating camellia anthracnose and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation of Bacillus subtilis Y13

[0031] The healthy leaves of common camellia oleifera are taken, separated, purified and preserved by conventional methods.

[0032] After the camellia oleifera was rinsed with water, an appropriate amount of healthy tissue was selected, weighed separately, cut into 5mm×5mm tissue pieces, soaked in 75% ethanol for 1min, then soaked in 0.1% mercuric solution for 40s-70s, and then placed Soak in 75% ethanol for 30s, take it out and rinse it with sterile water 4 times. Add 10ml of sterile water and a little sterilized quartz sand into a sterile mortar to grind into a homogenate, cover and let stand for 30 minutes, serially dilute the homogenate 10 times, and pipette 0.1ml to prepare After smearing evenly on a good NA plate, put it into an incubator for cultivation, record the amount of bacteria after 48 hours, and select according to the shape, color and size of the colony, purify and store on the plate, and separate 5 batches in total...

Embodiment 2

[0034] Screening of Bacillus subtilis Y13

[0035] Preliminary screening: use the confrontation culture method. Screening of endophyte antagonistic pathogenic fungi. Put the 6mm-diameter bacterium cake of Camellia anthracnose bacteria in the center of the plate, and then use the inoculation ring to pick up the suspension of the bacteria to be tested and inoculate 2 to 3 kinds of endophytic bacteria equidistantly (3cm from the center of the plate). Incubate in the dark at 25°C in an incubator, observe and record the presence and size of the bacteriostatic zone after 4 days, and repeat 3 times in total. Select 8 bacterial strains with a bacteriostatic zone width greater than or equal to 4mm for re-screening, followed by Y5, Y12, Y13, Y19, Y21, Y29, Y31, and Y35 for re-screening (see Table 1).

[0036] Table 1 Antibacterial effect of endophytic bacteria on Camellia oleifera anthracnose

[0037]

[0038] Re-screening: use fermentation method. After the 8 initially screened ...

Embodiment 3

[0042] Inhibitory Effect of Bacillus subtilis Y13 on Mycelia of Camellia oleifera Anthracnose

[0043] Put the 6mm-diameter bacterium cake of Camellia anthracnose bacteria in the center of the potato sucrose medium PDA plate, and then spot the antagonistic bacteria Y13 at an equal distance of 3cm, repeat three times, culture at 25-28°C, and measure the diameter of the inhibition zone after 4 days. Depend on figure 1 It can be seen that Bacillus subtilis Y13 has a strong antibacterial effect on Camellia oleifera anthracnose.

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Abstract

The invention discloses a Bacillus subtilis Y13 with the preservation number of CCTCC NO.M 208264, for effectively controlling oil tea anthrax, pertaining to the field of plant protection technology. At the same time, the invention discloses its separation method and applications, the Bacillus subtilis Y13 is obtained by flat plate facedown experiment screening, and can effectively inhibit the growth of oil tea anthrax bacteria, which has high proofing effect, good environment safety and favorable development and application prospects.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to an endogenous biocontrol bacterial strain for preventing and treating camellia oleifera anthracnose, in particular to a bacillus subtilis Y13 for effectively preventing and treating camellia oleifera anthracnose and its application. Background technique [0002] Camellia oleifera (Camellia oleifera) is a unique edible oil tree species in my country and one of the four largest woody oil tree species in the world. It has a history of utilization and cultivation for more than 2,000 years. It has wide adaptability and strong stress resistance, and is suitable for planting in vast red soil hilly areas. It mainly grows in the south, mainly in Hunan, Jiangxi, and Guangxi. Among them, Hunan Province is the largest camellia oleifera producing area and the largest camellia oil consumption area in China. Camellia oleifera is full of treasures and has high economic value. Accord...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 刘君昂周国英李河李琳宋光桃靳爱仙卢丽俐
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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