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Temporary immersion type culture method and culture device for dendrobe

A cultivation method and cultivation device technology, applied in the field of bioengineering, can solve problems such as unresolved, high ventilation requirements, increased cultivation costs, etc., and achieve the effects of reducing cultivation costs and simplifying cultivation conditions

Inactive Publication Date: 2015-08-12
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

By searching the prior art, it was found that Jiang Ping et al. (2013) invented a "sugar-free tissue culture method of Dendrobium officinale", and Xiao Yulan et al. (2012) invented "a sugar-free box-type plant tissue culture method". method", Wang Xilin et al. (2006) invented a "airponic plant sugar-free tissue culture device", these invention patents can reduce the pollution rate of Dendrobium aseptic tissue culture seedlings, but did not solve the problem of growing Dendrobium seedlings under natural outdoor conditions. , the question of the matrix required
In addition, Dendrobium has high requirements for root ventilation. In the above-mentioned patent, the plants are soaked in liquid for a long time, which will easily cause root rot; in addition, injecting carbon dioxide into the bottom of the box will increase the planting cost.

Method used

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  • Temporary immersion type culture method and culture device for dendrobe

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Embodiment Construction

[0017] The intermittent immersion culture method of dendrobium of the present invention comprises the steps:

[0018] 1. Sterilize the dendrobium seeds with mercuric chloride solution, then inoculate them into the seed germination medium, and cultivate them under indoor light; the seed germination medium is MS solid medium with 0.5 mg / L gibberellin added.

[0019] 2. After the aseptic seedlings take root, open the bottle cap and train the seedlings for 2 days;

[0020] 3. After seedling training, wash off the agar remaining in the roots, and then transfer to the intermittent submerged culture device for cultivation.

[0021] 4. At room temperature, place the intermittent immersion culture device in a cool place outside, avoiding direct sunlight. Inject the nutrient solution into the lower cavity of the intermittent immersion culture device, the formula of the nutrient solution is 1 / 2MS sugar-free medium with a large number of elements halved, then aseptically insert the dendr...

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Abstract

The invention discloses a temporary immersion type culture method and culture device for dendrobe. Rooted sterile dendrobe seedlings are transferred to a temporary immersion type culture device and are subjected to liquid culture under a natural condition, no sugars are added into culture solution, no carbon dioxide needs to be introduced, and carbon sources required for the growth of the dendrobe are from self photosynthesis. According to the method, a dendrobe culture condition is simplified, and a plantation cost is lowered.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to an intermittent immersion culture method and culture device of dendrobium. technical background [0002] Dendrobium is a precious nourishing Chinese herbal medicine in my country. It has the functions of invigorating qi and invigorating the stomach, nourishing kidney yin, and enhancing human immunity. It is listed as the top grade in "Shen Nong's Materia Medica". Due to the huge amount, it is facing two crises at present: the one is that the reserves and output of wild plant resources generally have a downward trend; Traditional Dendrobium cultivation techniques mainly include living tree inoculation cultivation and soilless substrate cultivation. The yield of live tree inoculation culture is too low; the substrate used in soilless substrate cultivation is mainly pine bark, which not only destroys valuable tree resources, but also increases the cost of planting. By searching the pri...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G31/02
CPCA01G31/00A01G31/02A01G2031/006Y02P60/21
Inventor 王贵荣
Owner SHANGHAI JIAO TONG UNIV
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