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Streptomyces and application thereof

A technology of streptomyces and pathogenic bacteria, applied in the field of microorganisms, to achieve the effects of good development and application prospects, easy industrial production, and fast reproduction speed

Active Publication Date: 2014-08-06
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the isolation of endophytic actinomycetes with biological control potential from medicinal plants, especially yangjiaoao, has not been reported at home and abroad.

Method used

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  • Streptomyces and application thereof
  • Streptomyces and application thereof
  • Streptomyces and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Screening and Identification of Antagonistic Endophyte SSD49

[0027] 1. Antagonistic endophyte SSD49 was isolated from Yangjiaoyu (collected from Danzhou Botanical Garden, Hainan). Specific steps are as follows:

[0028] Take the root tissue of the medicinal plant Yangjiaoao and air-dry it for 48 hours, treat it with 5% sodium hypochlorite for 10 minutes, soak it in an aqueous solution of 2.5g / 100ml sodium thiosulfate for 10 minutes, and treat it with an aqueous solution of ethanol with a volume percentage content of 75% for 5 minutes. Rinse with bacterial water several times, and finally soak in 10g / 100ml sodium bicarbonate aqueous solution for 10min. Under sterile conditions, use sterile scissors to cut the plant tissue into a tissue piece of about 1cm x 1cm x 1cm in length, width, and height. After 6 weeks, the strains with different colony morphology were picked, streaked and purified on the TWYE plate again, and finally the endophyte strain SSD49 was ...

Embodiment 2

[0058] Embodiment 2, the growth-promoting effect of antagonistic bacterial strain SSD49 on soybean and tomato

[0059] SSD49 was cultured with ISP2 medium (yeast extract 0.4g / 100ml, malt extract 1g / 100ml, glucose 0.4g / 100ml, and water) at 28°C for one week, then washed the spores on the plate with sterile water, Observe and calculate the spore concentration with a hemocytometer, and finally dilute to 10 with sterile water 7 cfu / ml. Soybean and tomato seed are planted in the plastic pot that sterilized soil is housed, treat to grow the antagonistic bacteria SSD49 spore liquid of 50ml after two true leaves (10 7 cfu / ml) in the rhizosphere soil, plant 2 plants per pot of soybeans, 3 plants per pot of tomatoes, 5 pots of each plant, as the experimental group of soybeans and tomatoes, and set the same volume of spore liquid without applying antagonistic bacteria SSD49 at the same time The same batch of soybeans and tomatoes replaced by sterile water were used as control group soy...

Embodiment 3

[0067] Embodiment 3, the control effect of antagonistic bacterial strain SSD49 to soybean sclerotinia

[0068] Choose plump soybean seeds, sow 2 seeds in every pot, and water 50ml antagonistic bacteria SSD49 spore liquid (concentration is 10 7 cfu / ml) (preparation method is the same as embodiment 2), as an experimental group, the same batch of soybeans is poured with sterile water 50ml as a control group, and each group of soybeans is inoculated with soybean sclerotinia pathogenic spore suspension (soybean sclerotinia) after 7 days of watering Preparation of pathogenic spore suspension: After culturing soybean sclerotinia pathogenic bacteria with PDA solid medium for one week at 28°C, rinse the spores on the lower plate with sterile water, calculate the spore concentration with a hemocytometer, and finally dilute to 10 7 cfu / ml)) 50ml at the base of seedling stems, and 5 pots were set in parallel for each group.

[0069] The incidence and disease index of soybean sclerotinia...

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Abstract

The invention discloses streptomyces and an application thereof. A preservation number of the streptomyces is CGMCC No.9034. The streptomyces is endophyte, has strong inhibiting effect to various plant pathogenic fungi, has many advantages in plant disease biological control, such as numerous amount, massive existence in and out of bodies of plants, diversification in action mode to pathogenic bacteria, rapid speed in propagation, strong root-colonizing capacity in and out of the bodies of the plants, promotion in the growth of the plants and the like and particularly has important significance of preventing and treating soybean sclerotinia sclerotiorum and poplar canker. Meanwhile, the streptomyces is simple in culture condition, is easy to preserve, is easy in industrial production and has good development and application prospect.

Description

technical field [0001] The invention relates to a strain of streptomyces and its application, in particular to a strain of streptomyces antagonizing plant pathogenic fungi and its application, belonging to the field of microorganisms. Background technique [0002] Plant fungal diseases are a major threat to food production and ecosystem stability in the world. At present, their control mainly depends on chemical control. However, the extensive use of chemical pesticides will lead to increased resistance of plant pathogenic fungi to pesticides. At the same time, due to improper or excessive use It will cause serious pollution to the environment. More and more studies have found that the use of beneficial microorganisms that can promote plant growth and inhibit phytopathogenic fungi for biological control is a feasible, environmentally friendly, cost-effective, and alternative strategy for chemical control. Many plant growth-promoting bacteria can pass through the endothelial...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P21/00C12R1/465
Inventor 马玉超刘晓瑜窦桂铭
Owner BEIJING FORESTRY UNIVERSITY
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