Bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and application thereof

A technology for resolving amyloliquefaciens and tobacco bacterial wilt, which is applied to Bacillus amyloliquefaciens B011 and its application fields, can solve the problems such as the lack of specific medicines for preventing and controlling tobacco bacterial wilt, the environment of bacterial resistance, and the impact on the control effect, etc. Good development prospects and application potential, no toxic side effects, good control effects

Active Publication Date: 2015-06-17
湖南省烟草公司永州市公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because tobacco bacterial wilt is a soil-borne bacterial disease, the surface soil absorbs chemical pesticides in the process of field application, which affects its control effect. At present, there is no specific agent for the control of tobacco bacterial wilt
Because bacterial wilt is mainly transmitted through soil, and its transmission route is hidden, long-term and large-scale application of chemical pesticides will easily cause the bacteria to develop drug resistance and cause environmental pollution.

Method used

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  • Bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and application thereof
  • Bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and application thereof
  • Bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] The separation method of Bacillus amyloliquefaciens B011 of the present embodiment, its separation steps are as follows:

[0046] (1) Sampling: in the tobacco field where tobacco bacterial wilt has occurred, select still growing healthy tobacco plant samples, wash, cut off the main root and lateral roots and carry out surface disinfection, get a sterilized sample, and set aside;

[0047] (2) Grinding: put 0.1 g of the spare sterilized sample in a sterilized mortar, add 10 ml of sterile water for grinding to obtain a grinding liquid, and set aside;

[0048] (3) Centrifugation: Take 1ml of the spare grinding solution and let it stand for 30 minutes, and then centrifuge it with a centrifuge at 7000r / min at 4°C to obtain the supernatant after centrifugation, and set it aside;

[0049] (iv) Purification: use sterile water for 10 minutes to reserve the supernatant -5 After gradient dilution, use the coating plate method on the NA medium, and after the colonies grow on the se...

Embodiment 3

[0055] The bacillus amyloliquefaciens B011 of application embodiment one gained prepares bacillus amyloliquefaciens B011 bacteriostatic agent:

[0056] First, prepare the NA medium slant, inoculate the Bacillus amyloliquefaciens B011 obtained in Example 1, put it into a 30°C incubator and cultivate it for 48 hours to obtain the slant bacteria, and set aside;

[0057] Next, configure NA liquid medium, divide into triangular flasks (250mL, 100mL liquid) before sterilization, sterilize at 121°C for 20min, and wash off the above-mentioned slanted bacteria directly with sterile water on the ultra-clean workbench after cooling. Insert it into the Erlenmeyer flask containing the above-mentioned sterilized NA liquid medium, culture in a shake flask, pH 7.0, 29°C, 170r / min, and cultivate for 48h to obtain shake flask culture bacteria for future use;

[0058] Next, configure the NA liquid medium according to the optimum fermentation condition combination formula optimized by the orthogo...

Embodiment 5

[0065] The Bacillus amyloliquefaciens B011 bacteriostatic agent of application embodiment three gained is to the inhibitory action test of multiple plant pathogenic bacteria:

[0066] Adopt plate confrontation method, test application embodiment three gained Bacillus amyloliquefaciens B011 bacteriostatic agent to tobacco black shank bacterium (P.parasitica), tobacco red spot bacterium (A.alternata Keissler), capsicum anthracnose bacterium (C.capsici), The antagonistic effects of seven pathogenic fungi, including P.capsici, C.gloeosprioides, P. oryzae and C.cuc-umerinum, were tested in PDA The central access of the tablet is 5mm 2 The target mycelium block of the target hyphae block is punched according to the method described in "Example 2" at the same distance from the target mycelium block. Add 20 μL of the Bacillus amyloliquefaciens B011 bacteriostatic agent prepared according to "Example 3" to two symmetrical wells, and inject sterile NA liquid medium into the other two s...

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Abstract

The invention discloses bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and an application thereof, and relates to the technical field of biological prevention and treatment of plant diseases, wherein the bacillus amyloliquefaciens B011 has the preservation number of CCTCC NO:M2014576; separation comprises the following steps: firstly, sampling; secondly, grinding, thirdly, centrifugation; fourth, purification, and fifthly, screening. The bacillus amyloliquefaciens B011 has the characteristics of relatively good antagonistic effect on tobacco bacterial wilt, good safety on the environment and easy industrialized production, not only can be used for preventing and treating tobacco bacterial wilt, but also can be used for preventing and treating P.parasitica, A.alternata keissler, also can be used for preventing and treating C.capsici, P.capsici, C.gloeosprioides, P.oryzae, C.cuc-umerinum and other pathogenic fungi.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to Bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and its application. Background technique [0002] Tobacco bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum (E.F.Smith)], which mainly invades from the roots. Tobacco bacterial wilt is mainly in the soil and the diseased plants in the soil It overwinters on the residue, enters the xylem through the wound of the rhizome or the root cap of the secondary root, and then spreads throughout the vascular bundle, causing severe wilting and eventually leading to tobacco withering and death. The disease occurs widely in tobacco-growing areas in southern my country, and tobacco bacterial wilt occurs more seriously in continuous cropping areas, which poses a great threat to tobacco production. [0003] Tobacco bacterial wilt is a soil-borne bacterial disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00C12R1/07
CPCA01N63/00C12R2001/07C12N1/205
Inventor 周向平陈武张洪波
Owner 湖南省烟草公司永州市公司
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